Performance of Pregnant Mare Serum Gonadotropin and Vitamin Mix in Inducing Striped Catfish Pangasianodon hypophthalmus Rematuration

Syifania Hanifah Samara, Citra Fibriana, Uthami Nagin Lestari, Agus Oman Sudrajat

Abstract


The research aimed to assess the performance of Pregnant Mare Serum Gonadotropin (PMSG) and Human Chorionic Gonadotropin (HCG) hormone injection combined with vitamin mix to induce the rematuration of Striped catfish Pangasianodon hypophthalmus broodstock. Three PMSG and HCG treatments of 5 and 2,5 IU; 10 and 5 IU;also 20 and 10 IU/kg of broodstock body weight dosage combined with vitamin mix addition to fish feed and one control was used with four replications. The broodstock was reared for four weeks and weekly sampling for GR and SGR as well as hormone injection were performed. For the following two weeks, the broodstock was observed for maturation. Artificial spawning was conducted for broodstock reaching gonadal maturity. Statistic analysis of observed variables showed insignificant differences among the three treatments in rematuration parameters. The rematuration rate resulted in a 100% pregnancy. Broodstock treated with 10 IU PMSG and 5 IU HCG/kg of spawner body weight was able to reach maturity and spawned with the larval survival rate on the fourth day at 84,81%, thus it can be concluded that 10 IU PMSG and 5 IU HCG/kg of body weight as the best dosage.

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Abidin, M. Z., Hashim, R., & Chong Shu Chien, A. (2006). Influence of dietary protein levels on growth and egg quality in broodstock female bagrid catfish (Mystus nemurus Cuv. & Val.). Aquaculture Research, 37(4), 416–418.

Allen, W. R., Hamilton, D. W., & Moor, R. M. (1973). The origin of equine endometrial cups. II. Invasion of the endometrium by trophoblast. The Anatomical Record, 177(4), 485–501.

Basuki, F. (1990). Pengaruh kombinasi hormon PMSG dan hCG terhadap ovulasi Clarias gariepinus (Burcell). Institut Pertanian Bogor.

Bates, R. O., Day, B. N., Britt, J. H., Clark, L. K., & Brauer, M. A. (1991). Reproductive performance of sows treated with a combination of pregnant mare's serum gonadotropin and human chorionic gonadotropin at weaning in the summer. Journal of animal science, 69(3), 894-898.

Bendich, A., Machlin, L. J., Scandurra, O., Burton, G. W., & Wayner, D. D. M. (1986). The antioxidant role of vitamin C. Advances in Free Radical Biology & Medicine, 2(2), 419–444.

Bui, T. M., Phan, L. T., Ingram, B. A., Nguyen, T. T. T., Gooley, G. J., Nguyen, H. V, … De Silva, S. S. (2010). Seed production practices of striped catfish, Pangasianodon hypophthalmus in the Mekong Delta region, Vietnam. Aquaculture, 306(1–4), 92–100.

Emata, A. C., Borlongan, I. G., & Damaso, J. P. (2000). Dietary vitamin C and E supplementation and reproduction of milkfish Chanos chanos Forsskal. Aquaculture Research, 31(7), 557–564. https://doi.org/10.1046/j.1365-2109.2000.00467.x

Fibriana, C. (2010). REKAYASA REMATURASI IKAN PATIN SIAM Pangasianodon hypophthalmus DENGAN KOMBINASI PENYUNTIKAN HORMON PMSG DAN HCG SERTA PENAMBAHAN VITAMIN MIX 100 mg/kg PAKAN. Institut Pertanian Bogor.

Gunasekera, R. M., Shim, K. F., & Lam, T. J. (1997). Influence of dietary protein content on the distribution of amino acids in oocytes, serum and muscle of Nile tilapia, Oreochromis niloticus (L.). Aquaculture, 152(1–4), 205–221.

Hasler, J. F., McCauley, A. D., Schermerhorn, E. C., & Foote, R. H. (1983). Superovulatory responses of Holstein cows. Theriogenology, 19(1), 83-99.

Herviani, I., Junior, M. Z., & Carman, O. (2003). Effect of Triiodothyronine in Different Dosages on Growth and Survival Rate of Giant Gouramy (Osphronemus gouramy Lac.). Jurnal Akuakultur Indonesia, 2(2), 61–65.

Kelautan dan Perikanan, K. (2018). Kelautan dan perikanan dalam angka 2017.

Koley, S., & Samanta, G. (2011). Effect of Dietary Organic Acids on Feed Quality, Performance, Gut Milieu and Blood Profile of Starter Khaki Campbell Ducks. Indian Journal of Animal Nutrition, 28(3), 325–330.

Lestari, U. N. (2010). Induksi rematurasi ikan patin siam dengan kombinasi penyuntikan hormon PMSG mix dan penambahan vitamin mix 200 mg/kg pakan. Institut Pertanian Bogor.

Matsuura, T., Sugimura, M., Iwaki, T., Ohashi, R., Kanayama, N., & Nishihira, J. (2002). Anti-Macrophage Inhibitory Factor Antibody Inhibits PMSG-hCG-Induced Follicular Growth and Ovulation in Mice. Journal of Assisted Reproduction and Genetics, 19(12), 591–595.

Miller, G. W., Ulatowski, L., Labut, E. M., Lebold, K. M., Manor, D., Atkinson, J., … Traber, M. G. (2012). The α-Tocopherol Transfer Protein Is Essential for Vertebrate Embryogenesis. PLoS ONE, 7(10), e47402. https://doi.org/10.1371/journal.pone.0047402

Palace, V. P., & Werner, J. (2006). Vitamins A and E in the maternal diet influence egg quality and early life stage development in fish: a review. Scientia Marina, 70(S2), 41–57.

Putra, H. F. E., Rahardjo, S. S. P., & Permana, A. (2017). Pemijahan Ikan Hias Botia (Chromobotia macracanthus Bleeker) Secara Buatan dengan Injeksi Hormon HCG (Human Chorionic Gonadothropin) dan LHRH-A (Luteinizing Hormone Releasing Hormone Analog). Journal of Aquaculture and Fish Health, 6(3), 101–106.

Rahardhianto, A., Abdulgani, N., & Trisyani, N. (2012). Pengaruh Konsentrasi Larutan Madu dalam NaCl Fisiologis Terhadap Viabilitas dan Motilitas Spermatozoa Ikan Patin (Pangasius pangasius) Selama Masa Penyimpanan. Jurnal Sains & Seni ITS, 1(1), 58–63. https://doi.org/10.12962/j23373520.v1i1.917

Rumimpunu, A., Andaki, J. A., & Manoppo, V. E. N. (2017). POTENSI PENGEMBANGAN USAHA BUDIDAYA IKAN PATIN ( Pangasius SP) DI DESA TATELU KABUPATEN MINAHASA UTARA. Akulturasi, 5(9), 713–716.

Samara, S. H. (2010). Rekayasa rematurasi ikan patin siam Pangasianodon hypophthalmus dengan penyuntikan hormon PMSG dan HCG serta penambahan Vitamin mix 300 mg/kg pada pakan. Institut Pertanian Bogor.

Slembrouck, J., Komarudin, O., Maskur, M., & Legendre, M. (2005). Technical Manual For Artificial Propagation Of The Indonesian Catfish, Pangasius djambal. (S. Sudarto, R. Gustiano, & J. Subagja, Eds.). Jakarta: IRD, BRPBAT, BRPB, BRKP.

Tahapari, E., & Dewi, R. R. S. S. P. (2013). PENINGKATAN PERFORMA REPRODUKSI IKAN PATIN SIAM ( Pangasianodon hypophthalmus ) PADA MUSIM KEMARAU MELALUI INDUKSI HORMONAL. Berita Biologi, 12(2), 203–209.

Tang, U. M., & Affandi, R. (2001). Biologi reproduksi ikan. Intimedia.

Waagbø, R. (2010). Water‐soluble vitamins in fish ontogeny. Aquaculture Research, 41(5), 733–744.

Yaron, Z., & Levavi-Sivan, B. (2011). Endocrine regulation of fish reproduction. In Encyclopedia of fish physiology: from genome to environment (Vol. 2, pp. 1500–1508). Academic Press San Diego, CA.

Zairin Jr, M. (2003). Endokrinologi dan perannya bagi masa depan perikanan Indonesia. Orasi Ilmiah Guru Besar Tetap Ilmu Fisiologi Reproduksi dan Endokrinologi Hewan Air Fakultas Perikanan dan Kelautan IPB. Bogor (Vol. 55).




DOI: http://dx.doi.org/10.23960/aqs.v7i2.p715%20-%20724

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