OPTIMALISASI SEL Saccharomyces cerevisiae UNTUK MENINGKATKAN PRODUKTIVITAS DAN EFISIENSI INDUSTRI ETANOL [Optimization of Saccharomyces cerevisiae Cell to Increase Productivity and Efficiency of Ethanol Industry]

Banon Rustiaty


The development of bioethanol as fuel substitution is believed to overcome the potency of the world energy crisis including Indonesia. The bioethanol development can be done by increasing the production capacity of the existing bioethanol factory plant by improving yeast culture for enhancing the performance of the fermentation process. This study was aimed at obtaining a method of optimizing the ability of Saccharomyces cerevisiae fermentation that can be applied by the alcohol industry in Indonesia for increasing factory productivity, thereby reducing the cost of producing alcohol. In this study, the adaptation of Saccharomyces cerevisiae Watei and Saccharomyces cerevisiae Hakken I were adopted in environment condition with high ethanol content up to 13%. The results showed that the yeast was able to grow in environments with high ethanol content with higher specific growth rate and larger cell size than those within the original yeast. This condition showed that adapted strains can overcome stress caused by high ethanol. These results promise the good performance yeasts with ability in growing and performing metabolic activities in high alcohol-containing environment conditions


optimization of Saccharomyces cerevisiae cell; productivity and efficiency; ethanol industry

Full Text:



Alexandre, H., I. Rousseaux, and C. Charpentier. 1994. Relationship between ethanol tolerance, lipid composition and plasma membrane fluidity in Saccharomyces cerevisiae and Kloeckera apiculata. FEMS Microbiol Lett. 124:17-22.

Attfield, P.V. 1997. Stress tolerance: the key to effective strains of industrial baker's yeast. Nat. Biotechnol. 15(13):1351-1357.

Barcelos, C.A., R.N. Maeda, L.M.M. Santa Anna, and N. Pereira. 2016. Sweet sorghum as a whole-crop feedstock for ethanol production. Biomass and Bioenergy 94:46–56.

Brandao, R.G., J.C. Camara Rosa, J.R. Nicoli, M.V.S. Almeida, A.P. do Carmo, H.T. Queiros, and I.M. Castro. 2014. Investigating acid stress response in different Saccharomyces strains. Review Article. Journal of Mycology. 2014: 1 – 9.

Caspeta, L., T. Castillo, and J. Nielsen. 2015. Modifying yeast tolerance to inhibitory conditions of ethanol production processes. Frontiers in Bioeng. and Biotech. 3: 1 – 15.

Dinh, T.N., K. Nagahisa, T. Hirasawa, C. Furusawa, and H. Shimizu. 2008. Adaptation of Saccharomyces cerevisiae cells to high ethanol concentration and changes in fatty acid composition of membrane and cell size. PloS ONE 3(7): 1-7.

Gallagher, W.P., C.Y. Winnie and H.S. Baumes. 2015. Energy balance for the corn-ethanol industry. United States Department of Agriculture. pp 1–21.

Gombert, A.K. and A.J.A. van Maris. 2015. Improving conversion yield of fermentable sugars into fuel ethanol in 1st generation yeast-based production processes. Current Opinion in Biotechnology 33:81–86.

Ismail, A.A. and M.M Ali. 1971. Selection of high ethanol yielding Saccharomyces I. ethanol tolerance and the effect of training in Saccharomyces cerevisiae Hansen. Folia Microbiology. 16 : 346-369.

Lloyd, D., S. Morrell, H.N. Carlsen, H. Degn, P.E. James, and C.C. Rowlands. 1993. Effects of growth with ethanol on fermentation and membrane fluidity of Saccharomyces cerevisiae. Yeast. 9(8): 825-833.

Reid, M. Lana, M.J. Morrison, X. Zhu, K.K. Jindal, and B.L. Ma. 2015. High stalk sugar corn: a potential biofuel crop for canada. Agronomy J. 107(2):475–85

Tesfaw, A., and F. Assefa. 2014. Current trends in bioethanol production by Saccharomyces cerevisiae: substrate, inhibitor reduction, growth variables, coculture, and immobilization. Review Article. International Scholarly Research Notices. 2014: 1 – 11.

Tjahyono, A.E., D.P. Meidiawati., dan T, Bambang. 1986. Pengujian toleransi berbagai galur Saccharomyces cerevisiae terhadap alkohol pada media molases. Laporan Teknis Unit Pelaksana Teknis Ethanol dan Proetein sel.

Varela, C., F. Pizarro, and E. Agosin. 2004. Biomass content governs fermentation rate in nitrogen deficient wine musts. Appl Ebviron Microbiol. 70: 3392-3400.

DOI: http://dx.doi.org/10.23960/jtihp.v23i2.97-102


  • There are currently no refbacks.