The Effect Of Antioxidant (Alpha-Tocopherol) Addition In Preservation Medium On Follicle Diameter And Oocyte Quality After Local Sheep Ovary Preservation

Authors

  • Najma Rika Faradana UNIVERSITAS PADJADJARAN
  • Rini Widyastuti UNIVERSITAS PADJADJARAN
  • Rangga Setiawan UNIVERSITAS PADJADJARAN

DOI:

https://doi.org/10.23960/jipt.v13i3.p702-714
Abstract View: 2

Abstract

This study aimed to evaluate the effect of adding the antioxidant Alpha-Tocopherol to the preservation medium on follicle diameter and oocyte quality of local sheep ovaries after 24 hours of storage at 4–5°C. The ovaries were divided into four treatment groups: P1 (dPBS), P2 (dPBS + 200 µM H₂O₂), P3 (dPBS + 200 µM H₂O₂ + 3 mM alpha-tocopherol), and P4 (dPBS + 3 mM alpha-tocopherol). The results showed no significant differences in follicle diameter changes among all treatments. The addition of Alpha-Tocopherol also did not demonstrate a notable effect in maintaining oocyte quality. Oocytes collected from all treatment groups exhibited relatively similar percentages of grade 1, 2, and 3. This study indicates that Alpha-Tocopherol does not provide a significant protective effect on follicle diameter or oocyte quality during short-term ovary preservation.

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Published

2025-11-01

How to Cite

Faradana, N. R., Widyastuti, R., & Setiawan, R. (2025). The Effect Of Antioxidant (Alpha-Tocopherol) Addition In Preservation Medium On Follicle Diameter And Oocyte Quality After Local Sheep Ovary Preservation. JURNAL ILMIAH PETERNAKAN TERPADU, 13(3), 702–714. https://doi.org/10.23960/jipt.v13i3.p702-714

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Articles