The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours

Sigit Bintara; Diah Tri Widayati; Widya Asmarawati; Riyan Nugroho Aji

doi:10.23960/jipt.v11i1.p34-40

The Effect of Lycopene Addition on the Semen Quality of Saanen Goats Stored at 5°C for 24 hours

Sigit Bintara, Diah Tri Widayati, Widya Asmarawati, Riyan Nugroho Aji

Abstract

In order to increase the livestock population, a good reproductive process was needed. Lycopene which belongs to carotenoid group has a dark yellow pigment. This study aimed to determine the effect of adding lycopene on the semen quality of Saanen goats. The research design used 4 treatments, P0 (egg yolk tris diluent) as control), P1 (egg yolk tris diluent + 2% lycopene), P2 (egg yolk tris diluent + 4% lycopene), and P3 (yolk tris diluent). egg + 6% lycopene). The quality of semen was observed after 24 hours of storage at 5℃. Macroscopic data were analyzed descreptively and the microscopic one was analyzed using a completely randomized design (CRD). The results showed that the addition of lycopene in egg yolk tris diluent had a significant (P<0.05) effect on the semen quality of Saanen goats. The results showed that the motility of spermatozoa in each treatment group (P0, P1, P2, and P3) was 60.1 ± 5.7%, 65.2 ± 4.3%, 62.4 ± 5.2%, and 60.6 ± 4.7%, respectively, viability were 65.3 ± 4.8, 73.5 ± 4.2, 70.4 ± 3.8, and 66.1 ± 5.1, respectively, abnormality were 17 ± 1.8%, 16 ± 1.8%, 18 ± 1.9%, and 16 ± 1.7%, respectively, and membrane integrity were 63 ± 3.3%, 70 ± 3.6 %, 67 ± 3.2%, and 62 ± 2.9%, respectively. Based on the results, it could be concluded that the lycopene addition improved the semen quality of Saanen goats compared to controls.

Keywords

Saanen goat; Semen quality; Lycopene; Egg yolk tris

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References

Alfa, N., S. Mustofa, and N. A. V. Irawati. 2019. Likopen, Antioksidan Eksogen yang Bermanfaat bagi Fertilitas Laki-laki. Medical Journal of Lampung University, 8(1): 237-241.

Arifulloh, I. Oktavianawati, I. N. A. Winata. 2016. Ektraksi Likopen dari Buah Tomat (Lycopersicum esculentum Mill.) dengan Berbagai Komposisi Pelarut. Berkala Sainstek, 4(1): 15-18

Astarto, N. W. 2014. Pengaruh Likopen Terhadap Kualitas dan Kadar Malondialdehid Spermatozoa yang Dipajankan pada Zalir Peritoneum Wanita dengan Endometriosis. Indonesian Journal of Applied Sciences, 4(3): 143-153. https://doi.org/10.24198/.v4i3.16819

Astuti, M.E. 2017. Pengaruh Penambahan Sari Buah Tomat (Solanum lycopersicum ) Sebagai Pengencer Alami Terhadap Kualitas Penyimpanan Spermatozoa Sapi Bali (Bos sondaicus). Bionature, Vol. 18(2): 129-139

Dewanto, H. N., Lisdiana, dan W. Isnaeni. 2017. Pengaruh Ekstrak Kulit Buah Rambutan terhadap Kualitas Sperma Tikus yang Terpapar Asap Rokok. Life Science, 6(2): 62-68

Durairajanayagam, D., A. Agarwal, C. Ong, dan P. Prashast. 2014. Lycopene and Male Infertility. Asian Journal of Andrology, 16(3): 420-425. http://10.4103/1008-682X.126384

Dutta, S., A. Majzoub, dan A. Agarwal. 2019. Oxidative stress and sperm function: A systematic review on evaluation and management. Arab J. Urol. 17(2): 87–97. DOI: 10.1080/2090598X.2019.1599624

Gunawan, I., D. N. D. I. Laksmi, dan I G. N. B. Trilaksana. 2012. Efektivitas Penambahan Β-Karoten dan Glutathion pada Bahan Pengencer Terhadap Motilitas dan Daya Hidup Spermatozoa pada Semen Beku Sapi. Indonesia Medicus Veterinus, 1(3): 385-393

Imran, M., Ghorat, F., Ul-Haq, I., Ur-Rahman, H., Aslam, F, Heydari, M., Shariati, M. A., Okuskhanova, E., Yessimbekov, Z., Thiruvengadam, M., Hashempur, M. H., and Rebezov, M. 2020. Lycopene as a Natural Antioxidant Used to Prevent Human Health Disorders. Antioxidants, 9(8): 706. DOI: 10.3390/antiox9080706

Irawati, N., D. D. Purwantini, dan A. Sodiq. 2019. Estimating Genetic Parameter of Saanen Goat Production Characteristics using Paternal Halfsib Correlation. Animal Production, 21(1): 16-21.

Khan, U. M., Sevindik, M., Zarrabi, A., Nami, M., Ozdemir, B., Kaplan, D. N., Selamoglu, Z., Hasan, M., Kumar, M., Alshehri, M. M., and Rad, J., S. 2021. Lycopene: Food Sources, Biological Activities, and Human Health Benefits. Hindawi Oxidative Medicine and Cellular Longevity, 2021. DOI: 10.1155/2021/2713511

Khandoker, M., N. Afini, dan A. Azwan. 2018. Productive and reproductive performance of Saanen goat at AZ-Zahra farm of Sandakan in Malaysia. Bang. J. Anim. Sci. 47 (1):1-1. DOI: 10.3329/bjas.v47i1.39395

Kumar, P. V. N., P. Elango, S. Asmathulla, and S, Kavimani. 2017. A Systematic Review on Lycopene and Its Beneficial Effects. Biomedical Pharmacology Journal, 10(4): 2113-2120. DOI: 10.13005/bpj/1335

Marawali, A., M. S. Abdullah, and Jalaludin. 2019. Efektivitas Suplementasi Filtrat Jambu Biji dalam Pengencer AirKelapa-Kuning Telur terhadap Kualitas Semen Cair Sapi Bali. Jurnal Veteriner, 20(1):20-29. DOI: 10.19087/jveteriner.2019.20.1.20

Masyitoh, H., T. W. Suprayogi, R. N. Praja, P. Srianto, S. P. Madyawati, and A. L. Saputro. 2018. Persentase Motilitas dan Viabilitas Spermatozoa Kambing Sapera dalam Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Before Freezing. Jurnal Medik Veteriner, 1(3):105-112. DOI: 10.20473/jmv.vol1.iss3.2018.105-112

Rosmaidar, Dasrul, dan T. M. Lubis. 2013. Pengaruh Penambahan Sari Buah Tomat Dalam Media Pengencer Terhadap Motilitas dan Viabilitas Spermatozoa Kambing Boer yang Disimpan pada Suhu 3–5°C. Jurnal Ilmiah Peternakan 1(1): 7-17

Sitaresmi, S. D., R. I’tishom, dan A. Mustika. Effects of Lycopene on Spermatozoa Morphology in Balb/C Mice Exposed to 2-Methoxyethanol (2-ME). Folia Medica Indonesiana, 53(4): 264-266. DOI: 10.20473/fmi.v53i4.7158

Syuhriatin. 2021. Efektivitas Antioksidan Likopen Pada Buah Tomat (Lycopercisum esculentum) Terhadap Normalitas dan Abnormalitas Spermatozoa Sapi Bali dengan Metode Swim up. Jurnal Bionature, 22(1):9-14. DOI: 10.35580/bionature.v22i1.20604

Sujana, D., D. Wardani, dan Nurul. 2020. Review Artikel: Potensi Likopen dari Buah Tomat (Solanum lycopersicum L) Sebagai Antiaging Topikal. Jurnal Insan Farmasi Indonesia, 3(1): 56-65. DOI: 10.36387/jifi.v3i1.479

Tabatabaei, S., R. Batavani, and E. Ayen. 2011. Effects of vitamin C addition to chicken semen on sperm quality during in vitro storage of semen. Vet. Res. Forum. 2(2):103-111.

Tisngati, U., N. I. Meifiani, D. C. N. Apriyani, dan Martini. 2019. Four Factors Experiments for Fixed Model in Completely Randomized Design. IOP Conf. Series: Journal of Physics: Conf. Series 1175: 1-9. DOI: 10.1088/1742-6596/1175/1/012152

Zuriati, Y., R. R. A. Maheswari, and H. Susanty. 2011. Karakteristik Kualitas Susu Segar and Yoghurt dari Tiga Bangsa Kambing Perah dalam Mendukung Program Ketahanan and Diversifikasi Pangan. Prosiding Seminar Nasional Teknologi Peternakan and Veteriner. Hal. 613-619.

DOI: http://dx.doi.org/10.23960/jipt.v11i1.p34-40