Antibiotic Resistance of Escherichia coli Isolate from Broiler Cecum and Organic Broiler Cecum

Authors

  • Septianita Eva Rozani Universitas Lampung
  • Maria Erna Kustyawati (SCOPUS ID: 57194526751) Universitas Lampung http://orcid.org/0000-0001-7980-1844
  • Dewi Sartika (SCOPUS ID: 6123776) Universitas Lampung http://orcid.org/0000-0003-4616-5030
  • Subeki Subeki Universitas Lampung
  • Tanto Pratondo Utomo Universitas Lampung

DOI:

https://doi.org/10.23960/jipt.v11i1.p41-50
Abstract View: 515

Keywords:

Antibiotic, Broiler, Cecum, Escherichia coli, Resistance

Abstract

This study aims to determine the level of antibiotic resistance of Escherichia coli bacterial isolates from broiler cecum and organic broiler cecum using the disc diffusion method on Muller-Hinton Agar as many as 10 cecum samples for each group.Collection of samples using purposive sampling method. Isolates suspected of being Escherichia coliwere further tested using Indole, Methyl Red Voges-Proskauer (MRVP), and Citrate (IMVIC) biochemical tests. E. coli isolates were further tested with the Antimicrobial Susceptibility Testing which aims to test antibiotic sensitivity. The results obtained from testing antibiotic resistance against Escherichia coli in broiler caecums showed a fairly high level of resistance to Gentamicin (70%), Ampicillin (100%), Cephalotin (100%), Nalixid Acid (86%), Streptomycin (80%), Erythromycin (100%), Oxytetracycline (80%), and the lowest was the antibiotic Chloramphenicol (30%), while the level of resistance in organic broilers showed Ampicillin (100%), Cefalotin (70%), Erythromycin (100%), the lowest resistance was Gentamicin (10%), Oxytetracycline (20%), Chlorampenicol (10%). Escherichia coli isolates obtained from the caecum of broiler and organic broiler showed a fairly high level of resistance. Conclusions of this study were resistance of Escherichia coli bacteria isolated from broiler cecum showed high results to almost all antibiotics except for chloramphenicol antibiotics, while resistance to Escherichia coli bacteria in organic broilers was lower than broilers but still showed a fairly high level of resistance, except for antibiotics Gentamicin, Nalixid acid , and chloramphenicol.

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Author Biographies

Septianita Eva Rozani, Universitas Lampung

Magister Teknologi Industri Pertanian

Maria Erna Kustyawati, (SCOPUS ID: 57194526751) Universitas Lampung

Food Microbiology

Dewi Sartika, (SCOPUS ID: 6123776) Universitas Lampung

Microbiology of Agricultural Products

Tanto Pratondo Utomo, Universitas Lampung

Agroindustry

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Published

2023-03-28

How to Cite

Rozani, S. E., Kustyawati, M. E., Sartika, D., Subeki, S., & Utomo, T. P. (2023). Antibiotic Resistance of Escherichia coli Isolate from Broiler Cecum and Organic Broiler Cecum. JURNAL ILMIAH PETERNAKAN TERPADU, 11(1), 41–50. https://doi.org/10.23960/jipt.v11i1.p41-50

Issue

Vol. 11 No. 1 (2023)

Section

Articles

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