The Effect of Different Types of Egg Yolk in Tris Diluent on the Quality of Frozen Semen of Brahman Cattle
DOI:
https://doi.org/10.23960/jipt.v12i2.p122-141
Keywords:
Egg yolk, Brahman cattle, Breed chicken, Frozen semen quality, Tris diluentAbstract
This study was conducted at the Regional Technical Service Unit for Artificial Insemination, Lampung District, Terbanggi Besar Subdistrict, Central Lampung Regency, Lampung Province, from January 25 to 30, 2023. The objective of this research was to determine the effect of different types of egg yolk and to identify the best type of egg yolk in tris diluent on the quality of frozen semen of Brahman cattle. The experimental design used was a Completely Randomized Design with three treatments: standard egg yolk, herbal egg yolk, and omega-3 enriched egg yolk, each with six replications. The data obtained were analyzed using ANOVA at the 5% and 1% significance levels, and if significant, further analyzed with Duncan’s Multiple Range Test. The results showed that the different types of egg yolk in tris diluent had no significant effect (P>0.05) on post-thaw spermatozoa abnormalities, had a significant effect (P<0.05) on post-thaw spermatozoa motility, and had a highly significant effect (P<0.01) on the percentage of live spermatozoa post-thawing. The conclusion of this study was that the addition of standard egg yolk and omega-3 enriched egg yolk in tris diluent has a better effect on the motility and percentage of live spermatozoa of Brahman cattle compared to the addition of herbal egg yolk.Downloads
References
Affandy, L., D.M. Dikman, dan Aryogi. 2007. Petunjuk Teknis Manajemen Perkawinan Sapi Potong. Pusat Penelitian dan Pengembangan Peternakan. Loka Penelitian Sapi Potong. Grati, Pasuruan. https://repository.pertanian.go.id/items/179dcb55-9b8a-4a99-a886-c44c19152bee
Amaliah, R. 2019. Perbandingan Pengencer Tris Kuning Telur Ayam Kampung dan Itik Terhadap Kualitas Semen Sapi Brahman Post-Thawing. Disertasi. Fakultas Peternakan Universitas Hasanuddin. Makassar. https://repository.unhas.ac.id/id/eprint/4848/
Arifiantini, R.I., dan T.L. Yusuf. 2006. Keberhasilan penggunaan tiga pengencer dalam dua jenis kemasan pada proses pembekuan semen Sapi Frisien Holstein. Majalah Ilmiah Peternakan, 9(3). https://ojs.unud.ac.id/index.php/mip/article/view/1718
[BSN] Badan Standardisasi Nasional. 2021. SNI 4869-1:2021 Semen Beku – Bagian 1: Sapi. Badan Standardisasi Nasional. Jakarta
[BIBD] Balai Inseminasi Buatan Daerah Provinsi Lampung. 2021. Standar Operasional Prosedur. Lampung Tengah
Bintara, S., D. Maharani, I.G. Suparta, J.H.P. Sidadolog, Sumadi, S. Eleuser, A. Batubara. 2015. Sperm Quality of Gembrong Goat in Bali, East Java and North Sumatera after Extended with Citrate-egg Yolk, Tris-egg Yolk and Andromed®. The 6th International Seminar on Tropical Animal Production Integrated Approach in Developing Sustainable Tropical Animal Production October 20-22, 2015, Yogyakarta. https://journal.ugm.ac.id/istapproceeding/article/view/30713
Ducha, N., T. Susilawati., Aulanniam., W. Sri, dan P. Mulyoto. 2012. Ultrastructure and fertilizing ability of limousin bull sperm afterstorage in CEP-2 extender with and without egg yolk. Pakistan Journal of Biological Sciences, 15(20): 979-985. DOI: https://doi.org/10.3923/pjbs.2012.979.985
Feradis. 2010. Bioteknologi Reproduksi pada Ternak. Alfabeta. Bandung. https://cvalfabeta.com/product/bioteknologi-reproduksi-pada-ternak/
Fitri, N.L., R.E. Susetyarini, L. Waluyo. 2016. Pengaruh ekstrak buah ciplukan (Physalis angulata L.) terhadap kadar SGPT dan SGOT mencit putih jantan (Mus musculus) hiperglikemia yang diinduksi aloksan sebagai sumber belajar biologi. Jurnal Pendidikan Biologi Indonesia. 2 (2): 180-187. DOI: https://doi.org/10.22219/jpbi.v2i2.3763
Gordon , M. H. 1990. The Mechanism Of Antioxidants Action In Vitro. Elsevier Applied Food Science Series. Springer. London. https://link.springer.com/chapter/10.1007/978-94-009-0753-9_1
Gusna., B. 2017. Pengaruh Ramuan Herbal Labio-1 terhadap Kualitas Interior Ayam Ras Petelur Strain Isa Brown. Skripsi. Fakultas Peternakan Universitas Hasanudin. Makasar.
Hafez, E.S.E. 2000. Semen evaluation in reproduction in farm animals. 7 th edition. USA.
Hufail, I. 2017. Kajian organoleptik dan aktifitas antioksidan pada telur berkelium selama penyimpanan. Tesis. Program Pascasarjana Universitas Pasundan. Bandung. https://repository.unpas.ac.id/14497/
Kurnia. A., Soeparna, R. I. Arifiantini, dan R. Hidayat. 2016. Fertilitas semen beku dalam tris kuning telur dan skim yang diberi omega-3 pada sapi Simmental dengan ransum berimbuhan seng dan selenium minimal. Jurnal Veteriner, 19(2):251-262. DOI: https://doi.org/10.19087/jveteriner.2018.19.2.251
Morel, D., C.G. Mina 1999. Equine Artificial Insemination. Wallingford. Cabi Pub. https://archive.org/details/equineartificial0000davi
Jannah, S.L., M. Lamid, M. Sukmanadi, M. A.A. Arif, S. Chusniati, I.S.
Hamid, R. Solfaine. 2022. Potensi pemberian probiotik terhadap peningkatan berat badan, konsumsi, dan konversi pakan ayam petelur fase pre layer. Media Kedokteran Hewan, 33(2): 96-104. https://doi.org/10.20473/mkh.v33i2.2022.96-104
Novita, R., T. Karyono, dan Rasminah. 2019. Kualitas Semen Sapi Brahman pada Persentase Tris Kuning Telur yang Berbeda. Jurnal Sain Peternakan Indonesia, 14(4): 351-358. DOI: https://doi.org/10.31186/jspi.id.14.4.351-358
Nurcholis, R.I. Arifiantini, M. Yamin. 2016. Kriopreservasi semen domba garutmenggunakan tris kuning telur yang disuplementasi omega-3 minyak Ikan Salmon. Jurnal Veteriner, 17(2): 309-315. https://ojs.unud.ac.id/index.php/jvet/article/view/22135
Rahman., Y.,G. 2021. Viabilitas Semen Sapi Simmental Pasca Ekuilibrasi dengan Menggunakan Pengencer Tris KuningTelur dan Dosis Gliserol yang Berbeda. Skripsi. Fakultas Pertanian dan Peternakan Universitas Islam Negeri Sultan Syarif Kasim Riua. Pekanbaru. https://repository.uin-suska.ac.id/53581/
Rianto. 2004. Pemetaan Sentra Potensi Unggulan Komoditas Peternakan dan Perikanan. Laporan Akhir. Kerjasama Badan Perencanaan Pembangunan Daerah Kabupaten Blora dengan Fakultas Peternakan. Universitas Diponegoro. Semarang.
Safitri, A. T. Sardjito, P.A. Wibawati, I. Mustofa, A.L. Saputro, R.A. Prastiya. 2018. Kualitas semen segar sapi rambon banyuwangi dalam pengencer tris kuning telur dan susu skim kuning telur. Jurnal Medik Veteriner, 1(3): 62-67. DOI: https://doi.org/10.20473/jmv.vol1.iss3.2018.62-67
Setiono, N., S. Suharyati, P.E. Santosa. 2015. Kualitas semen beku Sapi Brahman dengan dosis krioprotektan gliserol yang berbeda dalam bahan pengencer tris sitrat kuning telur. Jurnal Ilmiah Peternakan Terpadu, 3(2): 61-69. DOI: http://dx.doi.org/10.23960/jipt.v3i2.p%25p
Sinha, S., B.C. Deka, M.K. Tamulu, and B.N. Borgohain. 1992. Effect of equilibration period and glycerol level in tris extender of quality of frozen goat semen. Indian Vet. J. 69 : 1107-1110.
South, S., R.R.H. Rumende, A. Papu. 2023. Integritas membran spermatozoa manusia pada proses sexing dengan pemberian kuning telur. Jurnal Bios Logos, (13(3): 134-140. DOI: http://dx.doi.org/10.35799/jbl.v13i3.52182
Sudarmono, A.S dan Y.B. Sugeng. 2009. Sapi Potong. Penebar Swadaya. Jakarta.
Sulistiawati, D., M.N. Chayanto, U. Santoso, Zuprizal. 2000. Studi Komparatif Mutu dan Sifat Sensoris Telur Omega-3. Seminar Nasional Industri Pangan, 1: 66-76.
Supriyanto. 2016. Faktor-faktor yang mempengaruhi keberhasilan program inseminasi buatan (IB) pada ternak sapi potong. Jurnal Triton, 7(2): 69-84. https://jurnal.polbangtanmanokwari.ac.id/index.php/jt/article/view/34
Susilawati, T. 2011. Spermatology. UB Press. Malang. https://bookstore.ub.ac.id/shop/peternakan/spermatology/
Susilawati, T. 2011. Tingkat keberhasilan inseminasi buatan dengan kualitas dan deposisi semen yang berbeda pada sapi Peranakan Ongole. Jurnal Ternak Tropika, 12 (1): 15-24. https://ternaktropika.ub.ac.id/index.php/tropika/article/view/109
Toelihere, M.R. 1993. Inseminasi Buatan Pada Ternak. Penerbit Angkasa. Bandung. https://opac.perpusnas.go.id/DetailOpac.aspx?id=315628
Widiastuti, E. 2001. Kualitas semen beku sapi FH dengan penambahan antioksidan vitamin C dan E. Skripsi Fakultas Perternakan. Insitut Pertanian Bogor. Bogor. https://repository.ipb.ac.id/handle/123456789/13105
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