IMUNITAS NON-SPESIFIK DAN SINTASAN LELE MASAMO (Clarias sp.) DENGAN APLIKASI PROBIOTIK, VITAMIN C DAN DASAR KOLAM BUATAN
Abstract
Lele masamo (Clarias sp.) merupakan lele varian baru dan banyak diminati oleh petani ikan di Indonesia. Salah satu cara untuk meningkatkan produksi budidaya intensif lele masamo dapat dilakukan dengan penambahan dasar kolam buatan, penggunaan imunostimulan berupa vitamin C dalam pakan dan probiotik termodifikasi. Penggunaan dasar kolam buatan pada budidaya lele masamo berdampak pada perubahan imunitas non-spesifik berupa stres akibat perubahan lingkungan budidaya dan perubahannya teramati melalui pengamatan profil darah. Penelitian ini dilakukan untuk mempelajari pengaruh penambahan dasar kolam buatan terhadap kadar hematokrit, total leukosit, diferensial leukosit: limfosit, monosit dan neutrofil lele masamo yang diberi imunostimulan berupa probiotik dan vitamin C. Penelitian dilakukan selama 45 hari dan pengamatan profil darah dilakukan pada hari ke-0, hari ke-15, hari ke-30 dan hari ke-45. Hasil penelitian menunjukkan bahwa penambahan dasar kolam buatan berpengaruh terhadap kadar total leukosit dan monosit lele masamo (P<0,05). Penambahan dasar kolam buatan tidak berpengaruh nyata terhadap kadar hematokrit, limfosit dan neutrofil lele masamo (P>0,05). Sintasan selama budidaya menunjukan penambahan dasar kolam buatan lebih baik dibandingkan tanpa dasar kolam buatan tetapi tidak berbeda nyata (P>0,05).
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