IMUNOGENISITAS KOMBINASI VAKSIN INAKTIF WHOLE CELL Aeromonas salmonicida DAN VITAMIN C PADA IKAN MAS (Cyprinus carpio)

Authors

  • Nurma Jana Hazzulli
  • Agus Setyawan
  • Esti Harpeni

Abstract

Penambahan vitamin C dalam vaksin diketahui dapat meningkatkan imunogenisitas dan efektivitas vaksin.  Penelitian ini bertujuan untuk meningkatkan imunogenisitas vaksin inaktif whole cell A. salmonicida dengan penambahan vitamin C pada ikan mas (Cyprinus carpio).  A. salmonicida diinaktifasi dengan formalin 1,5 % (v/v). Vaksin I disuntikkan secara intra peritoneal (i.p) (107 sel / ikan) kepada ikan mas (ukuran ± 30gr).  Vaksin II (Booster) dilakukan 7 hari setelah vaksinasi I dengan metode dan dosis yang sama.  Pengambilan darah (Bleeding) dilakukan sebelum vaksinasi, 7 hari setelah vaksinasi I, dan 7 hari setelah vaksinasi II.  Analisis darah meliputi titer antibodi, hematokrit, dan total leukosit diamati setiap pengambilan sampel darah.  Hasil penelitian menunjukkan penambahan 1000 mg vitamin C dalam vaksin memiliki nilai titer antibodi, hematokrit, dan total leukosit paling tinggi dari perlakuan lainnya yaitu 28, 28%, 88.000 sel/mm3, secara berturut-turut pada pengamatan terakhir (7 hari setelah booster).

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Published

2015-02-02

How to Cite

Hazzulli, N. J., Setyawan, A., & Harpeni, E. (2015). IMUNOGENISITAS KOMBINASI VAKSIN INAKTIF WHOLE CELL Aeromonas salmonicida DAN VITAMIN C PADA IKAN MAS (Cyprinus carpio). E-Jurnal Rekayasa Dan Teknologi Budidaya Perairan, 3(2), 359–366. Retrieved from http://jurnal.fp.unila.ac.id/index.php/bdpi/article/view/647

Issue

Vol. 3 No. 2 (2015)

Section

Articles

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This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.