Muscle Micromorphology and Histomorphometry of Sumba Ongole Cattle (Bos indicus)

  • Chandraone P. Kefi Amtiran
    Laboratory of Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Veterinary Medicine, University of Nusa Cendana
  • Filphin A. Amalo
    Laboratory of Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Veterinary Medicine, University of Nusa Cendana
  • Inggrid T. Maha
    Laboratory of Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Veterinary Medicine, University of Nusa Cendana
  • Heny Nitbani
    Laboratory of Anatomy, Physiology, Pharmacology and Biochemistry, Faculty of Veterinary Medicine, University of Nusa Cendana
DOI: https://doi.org/10.23960/jipt.v9i3.p265-278
Keywords Histomorphometry, Micromorphology, Muscle, Sumba Ongole cattle
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Abstract

This study aims to knowing the micromorphology and histomorphometry of the sumba ongole cattle muscles. The sample used was 6 muscle samples consisted of the longissimus dorsi and bicep femoris muscles taken from three sumba ongole cattle slaughtered at the West Sumba slaughterhouse. Muscle tissue was fixed using 10% formalin and made histological preparations and hematoxylin-eosin (HE) staining. Histological preparations were observed using a light microscope with objective magnifications of 4, 10, 40, and 100 times. The results showed that the muscle micromorphology in the transverse section consisted of many fascicles formed by muscle fibers with cell nuclei at the edges, connective tissue, and intramuscular fat. Muscles in longitudinal sections are formed by muscle fibers with light dark stripes and connective tissue. The muscle fibers diameter, fascicle diameter and thickness of the connective tissue in the bicep femoris muscle is higher than the longissimus dorsi muscle. In contrast, the number of muscle fibers per fascicle is higher in the longissimus dorsi muscle. Muscle histomorphometry are strongly influenced by anatomical location and function of the muscles, as well as the presence of intramuscular fat.

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References

Agung, P. P., Anwar, S., Wulandari, S., Sudiro, A., Said, S., dan Tappa, B. 2015. The Potency of Sumba Ongole (SO) Cattle: A Study of Genetic Characterization and Carcass Productivity. Journal of the Indonesian Tropical Animal Agriculture, 40(2): 71-78.

Astruc, T. 2014. Connective Tissue: Structure, Function, and Influence on Meat Quality. Encyclopedia of Meat Sciences, 1: 321-328. Elsevier Ltd. DOI: 10.1016/B978-0-12-384731-7.00186-0.

Bogucka, J., Bogucki, M., dan Frymarski, M. 2015. Microstructure of the Masseter Muscle (Musculus masseter) in Cattle. Folia Pomer. Univ. Technol. Stetin., Agric., Aliment., Pisc., Zootech., 322 (36)4: 29-36.

Chriki, S., Gardner, G. E., Jurie, C., Picard, B., Micol, D., Brun, J., Journaux, L., dan Hocquette, J. 2012. Cluster Analysis Application Identifies Muscle Characteristics of Importance for Beef Tenderness. BMC Biochemistry 13(29): 1-11. <http://www.biomedcentral.com/1471-2091/13/29>.

Christensen, M., Purslow, P. P., dan Larsen, L. M. 2000. The Effect of Cooking Temperature on Mechanical Properties of Whole Meat, Single Muscle Fibres and Perimysial Connective Tissue. Meat Science 55: 301-307. .

Dubost, A., Micol, D., Picard, B., Lethias, C., Andueza, D., Bauchart, D., dan Listrat, A. 2013. Structural and Biochemical Characteristics of Bovine Intramuscular Connective Tissue and Beef Quality. Meat Science 95: 555-561. Elsevier Ltd. DOI: 10.1016/j.meatsci.2013.05.040.

Giro, T. M., Kulikovski, A. V., Giro, V. V., dan Mosolov, A. A. 2020. Microstructural Studies of Muscle Tissue of Lamb of Aborginal Breeds of the Volga Region. IOP Conf. Series: Earth and Environmental Science 548: 1-7. DOI: 10.1088/1755-1315/548/8/082082.

Ili, M. E., Lalel, H. D. J., dan Manu, A. E. 2016. Pengaruh Aras Energi Pakan dan Skor Kondisi Tubuh Terhadap Produksi dan Kualitas Fisik Daging Ternak Sapi Bali Betina Afkir. Jurnal Peternakan Indonesia, 18 (1): 1-12.

Kementerian Pertanian. 2014. Keputusan Menteri Pertanian Republik Indonesia Nomor 427/Kpts/Sr.120/3/2014 Tentang Penetapan Rumpun Sapi Sumba Ongole. Jakarta: Kementerian Pertanian Republik Indonesia.

Lawrie, R. A. dan Ledward, A. E. 2006. Lawries Meat Science, Seventh Edition. Woodhead Publishing Limited. Chapter 3-The structure and growth of muscle: 51-73.

Listrat, A., Lebret, B., Louveau, I., Astruc, T., Bonnet, M., Lefaucheur, L., Picard, B., dan Bugeon, J. 2016. Review Article: How Muscle Structure and Composition Influence Meat and Flesh Quality. The Scientific World Journal: 1-14. DOI: 10.1155/2016/3182746.

Mendrofa, V. A., Priyanto, R., dan Komariah. 2016. Sifat Fisik dan Mikroanatomi Daging Kerbau dan Sapi pada Umur yang Berbeda. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 4(2): 325-331.

Nuraini, H., Mahmudah, Winarto, A., dan Sumantri, C. 2013. Histomorphology and Physical Characteristics of Buffalo Meat at Different Sex and Age. Media Peternakan, 36(1): 6-13.

Nuraini, H., Aditia, E. L., dan Brahmantiyo, B. 2018. Meat Quality of Indonesian Local Cattle and Buffalo. Bovine Science, Chapter 5: 65-78. DOI: 10.5772/intechopen.79904.

Oksbjerg, N. dan Therkildsen, M. 2017. Myogenesis and Muscle Growth and Meat Quality dalam Purslow, P. P. New Aspects of Meat Quality: from Genes to Ethics. Woodhead Publishing dan Elsevier. Chapter 3: 33-62.

Pusat Data dan Sistem Informasi Pertanian, Kementerian Pertanian. 2019. Daging Sapi. Buletin Konsumsi Pangan, 10 (1): 66.

Rusdiana, S. 2019. Fenomena Kebutuhan Pangan Asal Daging Dapat Dipenuhi Melalui Peningkatan Usaha Sapi Potong di Petani. Journal on Socio-Economics of Agriculture and Agribusiness, 13(1): 61-83.

Rusono, N. 2015. Peningkatan Produksi Daging Sapi untuk Mewujudkan Kedaulatan Pangan Hewani. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner: 12-21.

Safitri, A., Priyanto, R., Adnyane, I K. M., dan Nuraini, H. 2018. Karakteristik Fisik dan Mikrostruktur Otot Semitendinosus pada Sapi Lokal dan Sapi Impor. Jurnal Veteriner, 19(4): 488-496.

Soeparno. 2015. Ilmu dan Teknologi Daging Edisi Kedua. Yogyakarta: Gadjah Mada University Press.

Sofyan, H., Satyaningtijas, A. S., Sumantri, C., Sudarnika, E., dan Agungpriyono, S. 2020. Muscle Fiber Area and Warner Bratzler Shear Force (WBSF) Value of Aceh Cattle Semitendinosus Muscle. IOP Conf. Series: Earth and Environmental Science 465: 1-5. DOI: 10.1088/1755-1315/465/1/012009.

Suwiti, N. K. 2008. Identifikasi Daging Sapi Bali dengan Metode Histologis. Majalah Ilmiah Peternakan, 11(1): 31-35.

Suwiti, N. K., Suastika, I P., Swacita, I. B. N., dan Besung, I N. K. 2015. Studi Histologi dan Histomorfometri Daging Sapi Bali dan Wagyu. Jurnal Veteriner, 16(3): 432-438.

Vasilenko, L. I., Grebenshchikov, A. V., Danyliv, M. M., Vasilenko, O. A., dan Ozherelyeva, O. N. 2019 Conference Paper: Features of Muscle Tissue Microstructure of Cattle in Industrial Agglomerations under the Environmental Pressure Conditions. International Cientific and Practical Conference, “AgroSMART-Smart solutions for agriculture”, KnE Life Sciences: 611-619. DOI: 10.18502/kls.v4i14.5648.

Wangko, S., dan Karundeng, R. 2014. Komponen Sel Jaringan Ikat. Jurnal Biomedik, 6 (3): S1-7.

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Published
2021-12-18
How to Cite
Amtiran, C. P. K., Amalo, F. A., Maha, I. T., & Nitbani, H. (2021). Muscle Micromorphology and Histomorphometry of Sumba Ongole Cattle (Bos indicus). JURNAL ILMIAH PETERNAKAN TERPADU, 9(3), 265–278. https://doi.org/10.23960/jipt.v9i3.p265-278