Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle

  • Tinda Afriani
    Universitas Andalas
  • Endang Purwati
    Universitas Andalas
  • James Hellyward
    Universitas Andalas
  • Jaswandi Jaswandi
    Universitas Andalas
  • Mangku Mundana
    Universitas Andalas
  • Anna Farhana
    Universitas Andalas
  • Adisti Rastosari
    (SCOPUS ID: 57208306771) Department of Animal Production Technology, Faculty of Animal Science, Universitas Andalas
DOI: https://doi.org/10.23960/jipt.v10i3.p264-276
Keywords FSHR gene, PCR, Pesisir cattle, SNP
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Abstract

The purpose of this study was to identify genetic diversity in the early exon 10 of the FSHR gene or follicle stimulating hormone receptor in Pesisir cattle. The blood samples used were 70 blood samples from female Pesisir cattle aged 2-5 years which were obtained from BPTU-HPT Padang Mengatas, West Sumatera. The isolated DNA was then amplified using a pair of primers L and R with PCR technique which produced a fragment with a length of 847 bp. The services of 1st Base Singapore are used for sequencing the amplified product. The results of the research on the identification of the early exon 10 FSHR gene in Pesisir cattle were found that there were 5 SNPs at positions --53T>C, +17A>G, +650C>T, +706A>C and +707 ins>A in some introns 9 to exon 10 first part. The results showed that the genotypic frequency of the Pesisir cattle population was in Hardy-Weinberg imbalance and polymorphisms were found in the early exon 10 FSHR gene diversity.

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2022-12-31
How to Cite
Afriani, T., Purwati, E., Hellyward, J., Jaswandi, J., Mundana, M., Farhana, A., & Rastosari, A. (2022). Identification of Single Nucleotide Polymorphism (SNP) in Early Exon 10 of Follicle Stimulating Hormone Receptor (FSHR) Gene in Pesisir Cattle. JURNAL ILMIAH PETERNAKAN TERPADU, 10(3), 264–276. https://doi.org/10.23960/jipt.v10i3.p264-276