Utilization of Various Diluents on the Quality of Frozen Semen from Saanen Goats
DOI:
https://doi.org/10.23960/jipt.v13i1.p179-193
Keywords:
AndroMed®, Frozen Semen, OvixCell®, Saanen Goat, Semen QualityAbstract
Saanen goats have low semen volume per ejaculation but high concentration. This study was conducted at the Singosari Center Artificial Insemination (SCAI), Malang Regency, East Java. The purpose of this study was to determine the effect of using various diluents on the quality of Saanen goat semen. The samples used consisted of 3 male Saanen goats with body weights of 32.5 kg, 43 kg and 44.5 kg with ages of 1.5-2 years. The use of various different diluents consisted of tris aminomethane egg yolk diluent (Treatment 0), AndroMed® (Treatment I) and OvixCell® (Treatment II). The research method used a laboratory experimental method using a Randomized Block Design (RAK). Data analysis using Analysis of Variance (ANOVA) if there is a significant or very significant difference then further analysis using Duncan's Multiple Range Test. The results showed that the use of various different diluents had no significant effect (P>0.05) on motility, viability and spermatozoa abnormalities. The research variables included volume, color, pH, odor, consistency, spermatozoa concentration, motility, viability and abnormality. The results showed that the use of various diluents was feasible to be used as diluents and was able to maintain normal motility>40%, spermatozoa survival and abnormality (<5%) against semen freezing.
Downloads
References
Adhyatma, M., N. Isnaini dan Nuryadi. 2013. Pengaruh Bobot Badan Terhadap Kualitas dan Kuantitas Semen Sapi Simmental. Jurnal Ternak Tropika, 14(2):53-62
Barek, M.E., K. Uly., W.M. Nalley., H.L.L. Bellu dan T.M. Hine. 2020. Pengaruh Penambahan Sari Wortel dalam Pengencer Sitrat Kuning Telur Terhadap Kualitas Spermatozoa Kambing Bligon. Jurnal Nukleus Peternakan, 7(2): 109-117
Berghe F.V.D., M.C.J. Paris., M.B. Briggs., W.K. Farstad and D.B.B.P. Paris. 2018. A Two-Step Dilution Tris-Egg Yolk Extender Containing Equax STM Significantly Improves Sperm Cryopreservation in the African Wild Dog (Lycaon pictus). Cryobiology. 80:18-25. https://doi.org/10.1016/j.cryobiol.2017.12.095
Elsheikh, A.S and N.S. Elhammadli. 2015. Semen Quality of Mature Crossbred Male Goats during Different Seasons. Journal of Agriculture and Veterinary Science. 8(9):1-5. https://doi.org/10.970/2380-08920105
Hendri, M., G. Riady dan R. Daud. 2017. Hubungan Lingkar Skrotum dan Konsentrasi Spermatozoa PadaKambing Peranakan Etawa (PE) Jantan. JIMVET, 2(1):41-50.
Iskandari, N.N., S.P. Madyawati., P.A. Wibawati., T.W. Suprayogi., R.A. Prastiya dan B. Agustono. 2020. Perbandingan Pengencer Tris Kuning Telur dan Susu Skim Kuning Telur Terhadap Persentase Motilitas, Viabilitas dan Integritas Membran Plasma Spermatozoa Kambing Sapera Pada Penyimpanan Suhu 5˚C. Jurnal Medik Veteriner, 3(2): 196-202
Istanty, A.S., M.A. Salim., N. Isnaini dan T. Susilawati. 2017. Pengaruh Penggantian Bovine Serum Albumin (BSA) Dengan Putih Telur Dalam Pengencer Dasar CEP-2 Kualitas Semen Kambing Boer Pada Simpan Dingin. Jurnal Ternak Tropika, 18(1):1-9
Kakati, U., S. Sinha., B.C. Deka., R.K. Biswas., M. Baruti., N. Nahardeka and R.S. Borah. 2019. Effect of Soybean Lecithin Based Extender and OviXcell® on Quality of Frozen Semen in Beetal, Sirohi and Assam Hill Goat. International Journal of Livestock Research. 9(1):95-109. https://doi.org/10.5455/ijlr.20180509033715
Khalil, W.A., M.A. El-Harairy., A.E.B. Zeidan., M.A.E. Hassan and O. Mohey-Elsaeed. 2018. Evaluation of Bull Spermatozoa during and After Cryopreservation:Structural and Ultrastructural Insight. International Journal of Veterinary Science and Medicine. 6: 49-56. https://doi.org/10.1016/j.ijvsm.2017.11.001
Kusumawati, E.D., H. Leondro., A.T. Nugroho., Krisnaningsih., T. Susilawati., N. Isnaini dan R. Widhad. 2016. Pengaruh dan Lama Simpan Semen Segar Terhadap Motilitas dan Abnormalitas Spermatozoa Kambing Peranakan Etawa (PE). Seminar Nasional Hasil Penelitian. 199-206
Lupitasari, F.B.I., R.I. Anwar., D.A. Mahari dan M.S. Ningrum. 2020. Pengaruh Penambahan Maltosa Pada Pengencer Berbasis Lesitin Dalam Mempertahankan Kualitas Semen Cair Kambing. Prosiding Seminar Nasional Teknologi Peternakan dan Veteriner. 20(20): 375-385
Mekasha, Y., A. Tegegne and H. Rodriguez-Martinez. 2007. Sperm Morphological Attributes in Indigenous Male Goats Raised Under Extensive Husbandry in Ethiopia. Animal Reproduction. 4(1):12-22
Nadri T., A. Towhidi A, S. Zeinoaldini,, F. Martínez-Pastor., M. Mousavi., R. Noei., M. Tar and A.M. Sangchesmeh. 2019. Lecithin Nanoparticles Enhance The Cryosurvival of Caprine Sperm. Theriogenology. 133: 38-44. https://doi.org/1016/j.theriogenology.2019.04.024
Nisfimawardah, L., A. Firmawati., M.N. Ihsan., T. Susilawati and S. Wahjuningsih. 2023. Semen Cryopreservation Quality and Sperm Kinematics of Saanen Goats Using Different Diluents. World’s Veterinary Journal. 13(2):300-309. https://dx.doi.org/10.54203/scil.2023.wvj3
Nubatonis, A., T.I. Purwaningsih., Y. Oki dan B. Doarce. 2022. Evaluasi Spermatozoa Domba Jantan Berekor Tipis yang Digembalakan di Lahan Kering. Jurnal Peternakan Indonesia. 24(1): 55-65
Perumal, P., S. Jai., A.K. De., D. Bhattacharya., A.K. Nahak., R. Vikram and E.B. Chakurkar. 2023. Seasonal Stress on Semen Quality Profiles, Seminal Biochemical and Oxidative Stress Atributes in Endangered Teressa Goat of Andaman and Nicobar Islands. Asian Pacific Journal of Reproduction. 12(6):288-298
Putri, R.F., D.H. Hermawan dan Suyadi. 2019. Kualitas Semen Cair Kambing Boer Selama Penyimpanan Suhu Ruang dengan Penambahan Ekstrak Daun Kemangi (ocimum sanctum). Prosiding Seminar Nasional teknologi Peternakan dan Veteriner. 334-344
Ratnawati, D., N. Isnaini dan T. Susilawati. 2017. Pemanfaatn CASA Dalam Observasi Motilitas Spermatozoa Semen Cair Sapi Madura Dalam Pengencer Berbeda. Jurnal Ilmu-Ilmu Peternakan. 27(1):80-95
Sumardani, N.L.G., K. Budaarsa., T.I. Putri dan A.W. Puger. 2019. Umur memengaruhi Volume Semen dan Motilitas Spermatozoa Babi Landrace di Balai Inseminasi Buatan Baturiti, Tabanan, Bali. Jurnal Veteriner. 20(3):324-329
Susilawati, T. 2011. Spermatologi. Malang: Universitas Brawijaya Press
Sun, L., W. Fan., C. Wu., S. Zhang., J. Dai and D. Zhang. 2020. Effect of Substituting Different Concentrations of Soybean Lecithin and Egg Yolk in Tris-based Extender on Goat Semen Cryopreservation. Cryobiology. 92:146-150. https://doi.org/10.1016/j.cryobiol.2019.12.004
Suprayogi, T.W., S. Susilowati., T. Hernawati., F.G. Hafidha., C.A. Wening and A.B. Purnawan. 2022. Improved Quality of Kambing kacang Sexing Frozen Semen with Additon of Green Tea Extract. Journal of Advanced Veterinary and Animal Research. 9(3):412-418. http://doi.org/10.5455/javar.2022.i609
Vargas, D.H., M.C. Escobar., D.A. Davila., J.L. Pérez., J.M.M. Torres., W.D.C. Maya and H.C. Cadavid. 2023. Conventional and Functional Evaluation of Semen in Male Dairy Goats. Veterinarska Stanica. 54(2): 129-136
Wajo, M.J. 2006. Peningkatan Kualitas Pengenceran dan Lama Penyimpanan Semen Ayam Kampung Melalui Penambhan Yolk Puyuh (Conturnix conturnix japonica). Jurnal Ilmu Peternakan dan Veteriner Tropis (Journal of Tropical Animal and Veterinary Science). 1(2): 38-44
Wajo, M.J., I. Widayati dan B.S. Ratlalaan. 2023. Penambahan Yolk Puyuh, Sari Pepaya (Carica papaya L.), Ringer’s Serta Lama Penyimpanan Pada Suhu 5℃ Terhadap Kualitas Spermatozoa Ayam KUB). JIPVET. 13(3): 160-161
Wurlina, W., M. Hariadi., E. Safitri., S. Susilowati and D.K. Meles. 2020. The Effcet of Crud Guava Leaf Tannins on Motility, Viability and Intact Plasma Membrane of Stored Spermatozoa of Etawa Crossbred Goats. Veterinary World. 13(3):530-537. https://doi.org/10.14202%2Fvetworld.2020.530-537
Yuan, Y., M. Yu., L. Chen., X. Ren., Y. Qu., A. Shari and G. Li. 2023. Comparative Analysis of Different Metabolies in Semen of Guanzhong Dairy Goats with Different Motility rates. Theriogenology. 210: 53-61. https://doi.org/10.1016/j.theriogenology.2023.07.009
Downloads
Published
How to Cite
Issue
Section
License
Jurnal Ilmiah Peternakan Terpadu(JIPT) is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).