REDUCTION OF PATHOGENIC BACTERIA DURING FERMENTATION OF MASIN BY PROTEASE AND BACTERIOCIN-PRODUCING LACTIC ACID BACTERIA
DOI:
https://doi.org/10.23960/aqs.v7i1.p629-636
Abstract View: 397
Abstract
Contamination of pathogenic and spoilage bacteria on fermentation process of seafood and fisheries product is a major concern on food safety. The aims of this study were isolating and applying the bacteriocin- and protease-producing lactic acid bacteria from the gastrointestinal tract of mud crab for starter culture of masin, a traditional fermented shrimp from East Java. This study consisted of characterization of lactic acid bacteria, the application on the fermentation process and microbial analysis. Ninety-four isolates were isolated from mud crab was screened for the bacteriocin and protease producing as well as characterized by pH, salinity and biochemical. Isolate IKP-29 was exhibited strong protease and bacteriocin activity. Application of Isolate IKP-29 on masin fermentation showed that sharp reduction of Escherichia coli, Vibrio sp. and lactic acid bacteria counted. This study suggested that the application of lactic acid bacteria which producing bacteriocin and protease improved the food safety of traditional fermented fish.Downloads
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Balcazar, J.L., de Blas, I., Ruiz-Zarzuela, Il., Vendrell, D., & Calvo, A.C., 2007, Changes in intestinal microbiota and humoral immune response following probiotic administration in brown trout (Salmo trutta), Br. J. Nutr., 97, 522-527
Dalie, D.K.D., Deschamps, A.M., & Rickhard-Forget, F., 2010, Lactic acid bacteria-potential for control of mould growth and mycotoxins; a review. J. Food Control, Vol. 21, hal. 370-380
De Souza, E.L., da Silva, C.A. & de Sousa, C.P., 2005, Bacteriocins: molecules of fundamental impact on the microbial ecology and potential food biopreservatives, Brazilian Archives of Biology and Technology, Vol. 48, hal. 559-566
Eijsink, V.G.H., Axelsson, L. , Diep, D.B., Havarstein, L.S., Holo, H., & Nes, I.F., 2002, Production of class ii bacteriocins by LAB; an example of biological warfare and communication, Antonie van Leeuwenhoek, Vol. 81, hal. 639-654
Hwanhlem, N., Buradaleng, S., Wattanachant, S., Benjakul, C., Tani, A., & Maneerat, S., 2010, Isolation and screening of lactic acid bacteria from Thai traditional fermented fish (Plasom) and production of plasom from selected strains, J. Food Control, Vol. 22, hal. 401-407
Kopermsub, P. & Yunchalard, S., 2010, Identification of lactic acid bacteria associated with the production of plaa-som, a traditional fermented fish product of Thailand, Int. J. Microbiol., Vol. 138, hal. 200-204
Linscott, A.J., 2011, Food-Borne Illnesses. Clin. Microbiol. Newsletter, Vol.33, hal. 41-45
Ludwig, W. K-H. Schleifer, & W.B. Whitman. Lactobacillales ord. nov. Bergey Systematic bacteriology Vol. 3. London. Springer.465-511
Mohammed, M., El-Aziz, H.A., Omran,N., Anwar, S., Awad,S., & El-Soda, M., 2009, Rep-PCR characterization and biochemical selection of lactic acid bacteria isolated from the delta area of Egypt, Int. J. Food Microbiol., Vol. 128, hal. 417–423
Moraes, P.M., Perin, L.M., Ortolani, M.B.T., Yamazi, A.K., Vicosa, G.N., & Nero, L.A., 2010, Protocols for the isolation and detection of lactic acid bacteria with bacteriocinogenic potential, LWT-Food Science and Technology, Vol. 43, hal. 1320-1324
Moroni, A.V., Arendt, E.K. & Dal Bello, F., 2010, Biodiversity of lactic acid bacteria and yeasts in spontaneously-fermented buchwheat and teff sourdoughs, J. Food Microbiology, Vol. 28, hal. 497-502
Pramono, Heru, 2013, Isolasi bakteri asam laktat penghasil bakteriosin sebagai bahan pengawet produk perikanan, Prosiding MPHPI, UNDIP, Semarang.
Pramono, H., Suciati, P. and Andriyono, S., 2015. Isolation of Lactic Acid Bacteria That Produce Protease and Bacteriocin-Like Substance From Mud Crab (Scylla sp.) Digestive Tract (Isolasi Bakteri Asam Laktat yang Menghasilkan Protease dan Senyawa Bacteriocin-Like dari Saluran Pencernaan Kepiting. ILMU KELAUTAN: Indonesian Journal of Marine Sciences, 20(1), pp.33-37.
Putra, T.F., Suprapto, H., Tjahjaningsih, W. and Pramono, H., 2018, April. The antagonistic activity of lactic acid bacteria isolated from peda, an Indonesian traditional fermented fish. In IOP Conference Series: Earth and Environmental Science(Vol. 137, No. 1, p. 012060). IOP Publishing.
Schnürer, J. and Magnusson, J., 2005. Antifungal lactic acid bacteria as biopreservatives. Trends in Food Science & Technology, 16(1-3), pp.70-78.
Settani, L & Corsetti, A., 2008, Application of bacteriocins in vegetable food bipreservation, Int. J. Food Microbiol., Vol. 121, hal. 123-138
Speranza, B., Racioppo, A., Beneduce, L., Bevilacqua, A., Sinigaglia, M. and Corbo, M.R., 2017. Autochthonous lactic acid bacteria with probiotic aptitudes as starter cultures for fish-based products. Food microbiology, 65, pp.244-253.
Sugita, H., Okano, R., Suzuki, Y., Iwai, D., Mizukami, M., Akiyama, N., & Matsuura, S., 2002, Antibacterial ability of intestinal bacteria from larval and juvenile Japanese flounder against fish pathogen. Fish Sci., 68: 1004-1011
Talpur, A.D., Memon, A.J., Khan, M.I., Ikhwanuddin, M., Danish-Daniel, M.M., & Abol-Munafi, A.B., 2011, Isolation and screening of lactic acid bacteria from the gut of blue swimming crap, P. pelagicus, an in vitro inhibition assay and small scale in vivo model for validation of isolates as probiotics, J. Fish. Aquat. Sci.
Udhayashree, N., Senbagam, D., Senthilkumar, B., Nithya, K., Gurusamy, R., 2012, Production of bacteriocin and their application in food products, Asian Pac. J. Trop. Biomed. Vol. 2, hal: 406-410
Willey, J.M., Sherwood, L.M., & Woolverton, C.J., 2009, Prescott’s Principles Of Microbiology. Boston: McGraw-Hill Higher Education
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