INDUKSI KALUS KRISAN (Chrysanthemum morifolium) DENGAN PENAMBAHAN BERBAGAI KOMBINASI PIKLORAM DAN BAP

Sabrina Nur Farikha; Noor Aini Habibah

doi:10.23960/ja.v24i2.11197

Authors

Sabrina Nur Farikha Universitas Negeri Semarang
Noor Aini Habibah Universitas Negeri Semarang

DOI:

https://doi.org/10.23960/ja.v24i2.11197

Abstract View: 19

Abstract

Chrysanthemum (Chrysanthemum morifolium) contains secondary metabolites that are used as anticancer, anti-inflamantory, antioxidant, and antibiotic agents. In vitro techniques or tissue culture are considered capable of producing metabolites through callus culture with the addition of growth regulators (GRs). The objective of this study was to analyzed the effect of the type and concentration of Picloram and BAP on the formation of chrysanthemum callus. This study used a Completely Randomized Design (CRD) with two factors: treatment, consisting of the type of PGR (Picloram and BAP), and concentration, consisting of four treatment levels: 0 ppm, 1 ppm, 2 ppm, and 3 ppm. Quantitative data, including growth time, percentage, fresh weight and dry weight of callus, were analyzed using IBM SPSS Statistic 27, while qualitative data, including color and texture morphology of callus, were analyzed descriptively. The result show that Picloram has a significant effect on all observed parameters. The addition of BAP only affect the fresh weight of the callus, while the combination of Picloram and BAP affect both the fresh and dry weight of the callus. A Picloram concentration of 3 ppm was the most optimal treatment for all parameters, and the combination of 3 ppm Picloram + 3 ppm BAP produced the highest fresh and dry weights of the callus. The callus formed was predominantly yellowish-green in color with a crumbly texture. These findings indicate that the combination of 3 ppm Picloram + 3 ppm BAP is effective in increasing the efficiency of callus induction in C. morifolium.

Key words: BAP,callus, chrysanthemum, picloram

Downloads

Download data is not yet available.

References

Anggraeni, D., Ismaini, L., Surya, M. I., Rahmi, H., & Saputro, N. W. (2022). Inisiasi Kalus Daun Talinum triangulare (Jacq.) Willd pada Beberapa Kombinasi Konsentrasi Zat Pengatur Tumbuh 2,4-Dichlorophenoxyatic Acid dan Benzyl Adenine. Agrikultura, 33(3), 276. https://doi.org/10.24198/agrikultura.v33i3.40540

Apriliani, E., Widyajayantie, D., Hidayah, U. F., & Yudha, Y. S. (2023). Perbanyakan Tanaman Chrysanthemum pada Kondisi Fotoautotropik Secara in Vitro. Agriculture and Biological Technology, 1(1), 1–9. https://doi.org/10.61761/agiotech.1.1.1-9

Ariani, R., Anggraito, Y. U., & Rahayu, E. S. (2016). Respon Pembentukan Kalus Koro Benguk (Mucuna pruriens L.) Pada Berbagai Konsentrasi 2,4-D dan BAP. Jurnal MIPA, 39(1), 20–28.

Arieswari, N. N. N., Astarini, I. A., Astiti, N. P. A., & Pramana, J. (2018). In Vitro Callus Induction of “Shiraz” Grape (Vitis vinifera L.) Using Different Medium And Growth Regulator Combination. International Journal of Biosciences and BiotehcnologyI, 6(1), 3. https://doi.org/10.24843/IJBB.2018.v06.i01.p03

Azmi, F. N., & Damanik, R. I. (2024). Analysis of secondary metabolites through callus cell suspension culture of black cumin ( Nigella sativa L.) with PGR combination on MS media. IOP Conference Series: Earth and Environmental Science, 1302(1). https://doi.org/10.1088/1755-1315/1302/1/012043

Bano, A. S., Khattak, A. M., Basit, A., Alam, M., Shah, S. T., Ahmad, N., Gilani, S. A. Q., Ullah, I., Anwar, S., & Mohamed, H. I. (2022). Callus Induction, Proliferation, Enhanced Secondary Metabolites Production and Antioxidants Activity of Salvia moorcroftiana L. as Influenced by Combinations of Auxin, Cytokinin and Melatonin. Brazilian Archives of Biology and Technology, 65, 1–16. https://doi.org/10.1590/1678-4324-2022210200

Budi, R. S. (2020). Uji Komposisi Zat Pengatur Tumbuh Terhadap Pertumbuhan Eksplan Pisang Barangan (Musa paradisiaca L.) Pada Media MS Secara in vitro. BEST Journal (Biology Education, Sains and Technology), 3(1), 101–111. https://doi.org/10.30743/best.v3i1.2475

Erlangga, W., Yelli, F., Yusnita, & Utomo, S. D. (2025). Embriogenesis Somatik Ubi Kayu (Manihot esculenta Crantz.) Klon Waxy pada Beberapa Jenis dan Konsentrasi Auksin 2,4-D atau Picloram dan NAA. Jurnal Agrotropika, 24(1), 88–98.

Fadlia Julianti, R., Nurchayati, Y., Setiari, N., & Studi Biologi, P. (2021). Pengaruh Konsentrasi Sukrosa dalam Medium MS terhadap Kandungan Flavonoid Kalus Tomat (Solanum lycopersicum syn. Lycopersicum esculentum). Journal of Biological Sciences, 8(1), 141–149. https://doi.org/10.24843/metamorfosa.2020.v08.i01.p015

Gantait, S., & Mahanta, M. (2021). Picloram-induced enhanced callus-mediated regeneration, acclimatization, and genetic clonality assessment of gerbera. Journal of Genetic Engineering and Biotechnology, 19(1), 1–8. https://doi.org/10.1186/s43141-021-00269-1

Ginaris, R. P. (2023). Uji Aktivitas Masker Wajah Peel-off dari Ekstrak Bunga Krisan (Chrysanthemum cinerariaefolium (Trevir.) Vis.) Sebagai Antioksidan. Jurnal Kesehatan Tujuh Belas (Jurkes 17), 4(2), 248–253.

Gundala, B. T., Kurniawan, T., & Halimursyadah, H. (2018). Pengaruh konsentrasi auksin dalam hydropriming benih cabai yang berbeda tingkat kadaluarsa terhadap viabilitas benih. Jurnal Ilmiah Mahasiswa Pertanian, 3(4), 159–167. https://doi.org/10.17969/jimfp.v3i4.9378

Hariyati, M., Bachtiar, I., & Sedijani, P. (2016). Induksi Kalus Tanaman Krisan (Chrysanthemum morifolium) Dengan Pemberian Benzil Amino Purin (BAP) dan Dichlorofenoksi Acetil Acid (2,4 D). Jurnal Penelitian Pendidikan IPA, 2(1). https://doi.org/10.29303/jppipa.v2i1.37

Humaira, A., & Amien, S. (2019). Induksi Kalus Lima Kultivar Seledri (Apium graveolens L.) Dengan Sukrosa dan Berbagai Konsentrasi Maltosa. Agrin, 23(1), 1. https://doi.org/10.20884/1.agrin.2019.23.1.413

Jannah, R. (2016). Pengaruh Pemberian Elisitor Cu2+ Terhadap Kalus Artemisia vulgaris dalam Upaya Penyediaan Artemisinin sebagai Antimalaria. Pros. Sem. Nas. Mas. Biodiv. Indon, 2(2010), 155–158. https://doi.org/10.13057/psnmbi/m020206

Marlina, A., & Widiastuti, E. (2021). Studi Awal Pembuatan Bio-Insektisida dari Bunga Krisan (Chrysantemum morrifolium). Prosiding The 12th Industrial Research Workshop and National Seminar, 4–5.

Na’ilil Aulia, S., & Habibah, N. A. (2024). Callus Induction from Stem Explants of Binahong (Anredera cordifolia (Ten.) Steenis) with the Addition of Picloram and BAP. Journal of Biology and Applied Biology, 7(1), 13–22.

Perianez-Rodriguez, J., Manzano, C., & Moreno-Risueno, M. A. (2014). Post-embryonic organogenesis and plant regeneration from tissues: Two sides of the same coin? Frontiers in Plant Science, 5(MAY). https://doi.org/10.3389/fpls.2014.00219

Putri, A. B. S., Hajrah, H., Armita, D., & Tambunan, I. R. (2021). Teknik kultur jaringan untuk perbanyakan dan konservasi tanaman kentang (Solanum tuberosum L.) secara in vitro. Filogeni: Jurnal Mahasiswa Biologi, 1(2), 69–76. https://doi.org/10.24252/filogeni.v1i2.23801

Ruswaningsih, F. (2007). Pengaruh Konsentrasi Ammonium Nitrat dan BAP terhadap Pertumbuhan Eksplan Pucuk Artemisia annua L. pada Kultur In Vitro. Universitas Sebelas Maret, Surakarta.

Sari, Y. P., Kusumawati, E., Saleh, C., Kustiawan, W., & Sukartingsih, S. (2018). Effect of sucrose and plant growth regulators on callogenesis and preliminary secondary metabolic of different explant Myrmecodia tuberosa. Nusantara Bioscience, 10(3), 183–192. https://doi.org/10.13057/nusbiosci/n100309

Sekar, A. A., Restiani, R., & Prasetyaningsih, A. (2023). Induksi Kalus dari Eksplan Nodus Stelecocharpus burahol (Blume) Hook. f & Thomson sebagai Upaya Konservasi In Vitro. BIOTIKA Jurnal Ilmiah Biologi, 21(1), 27–35. https://doi.org/10.24198/biotika.v21i1.42869

Setiawati, T., Ayalla, A., Witri, A., & Raya Bandung-Sumedang Km, J. (2019). Induksi Kalus Krisan (Chrysanthemum morifolium Ramat.) dengan Penambahan Berbagai Kombinasi Zat Pengatur Tumbuh (ZPT). Jurnal EduMatSains, 3(2), 119–132.

Setiawati, T., Zazuli, S. F., Annisa, A., Nurzaman, M., & Irawan, B. (2020). Pengaruh Polyethylene Glycol (PEG) Terhadap Kadar Kuersetin Kultur Chrysanthemum morifolium Ramat pada Kondisi Pencahayaan Berbeda. Al-Kauniyah: Jurnal Biologi, 13(1), 116–127. https://doi.org/10.15408/kauniyah.v13i1.13688

Shofiyani, A., & Purnawanto, A. M. (2017). Pertumbuhan Kalus Kencur (Kaemferia galanga L) Pada Komposisi Media Dengan Perlakuan Sukrosa Dan Zat Pengatur Tumbuh (2,4 D dan Benzil Aminopurin). Agritech, 19(1), 55–64.

Smith, A. J., Oertle, J., Warren, D., & Prato, D. (2016). Quercetin: A Promising Flavonoid with a Dynamic Ability to Treat Various Diseases, Infections, and Cancers. Journal of Cancer Therapy, 07(02), 83–95. https://doi.org/10.4236/jct.2016.72010

Sudrajad, H., & Wijaya, N. R. (2019). Pengaruh Kinetin dan NAA Terhadap Induksi Kalus Pule Pandak (Rauvolfia serpentina (L.) Benth. ex Kurz). Jurnal Tumbuhan Obat Indonesia, 12(2), 68–74. https://doi.org/10.22435/jtoi.v12i2.1691

Surya, R. S., Gustian, & Zainal, A. (2018). Induksi Kalus Tanaman Kakao (Theobroma cacao L.) pada Beberapa Konsentrasi Picloram Secara In-Vitro Calus. Perhimpunan Ilmu Pemuliaan Tanaman (PERIPI). http://repo.unand.ac.id/27536/

Wahyuni, A., Satria, B., & Zainal, A. (2020). Induksi Kalus Gaharu dengan NAA dan BAP Secara In Vitro Callus Induction of Agarwood Using NAA and BAP In Vitro. Jurnal Penelitian Agronomi, 22(1), 39–44.

Wardani, D. K. (2020). Induksi Kalus Tanaman Nilam (Pogostemon cablin Benth) Dengan Pemberian Konsentrasi Auksin Jenis 2,4-D (Dichlorophenoxy Acetic Acid) Dan Pikloram. Jurnal Indonesia Sosial Sains, 1(5).

Wibowo, F., Armaniar, & Asmaq, N. (2023). Perbanyakan Vegetatif Tunas Mikro Anggrek Dendrobium (Dendrobium sp) Secara In Vitro Dengan Pemberian BAP dan Arang Aktif. Jurnal Pertanian Agros, 25(1), 910–916.