INDUKSI MUTASI DENGAN IRADIASI SINAR GAMMA DAN SELEKSI IN VITRO UNTUK MENDAPATKAN EMBRIO SOMATIK KACANG TANAH YANG TOLERAN POLIETILENA GLIKOL

A. Farid Hemon

Abstract


The objectives of this experiment were to evaluate effectiveness of Gamma ray to somatic embryos culture to increase frequency of obtaining polyethylene glycol (PEG) insensitive somatic embryos (SE) of cv. Local Bima peanut. The experiment was inisiated with induction of peanut SE. Mutation induction was done on SE culture. Somatic embryos that have been irradiated with Gamma ray were selected in medium containing polyethylene glykol 15% (PEG 6000). Results of the experiment achieved, namely: 1) Gamma ray dosage affected peanut SE growth. Higher Gamma ray dosage used, it was more inhibited peanut SEs growth. Use of IS Gy dosage gave SEs growth better than up dosage, 2) embriogenic calli irradiated with Gamma ray dosage 15 and 20 Gy showed high SE proliferation, high number of SE per explant, high SE total when selected in medium containing PEG 15%, and lower SE growth occured on SE calli without irrardion and dosage 25 Gy.

  Key words: Mutation, Gamma ray


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References


Ahloowalia, B. S. Maluszynsky M. 2001. Induce mutations — A new paradigm in plant breeding. Euphytica 118: 67-173.

Anonimous. 1997. Irradition of horticultural crops at Iowa State Univeristy. Hort. Sd 32(4): 582-585.

Gamborg, O. L., Miller R. A., and Ojima K. 1968. Nutrient requirement of suspension cultures of soybean root cells. Exp. Cell Res. 50:15 1-158.

Gaul, H. 1977. Mutagent effect in the first generation after seed treatment. In: Manual on Mutation Breeding, Technical Reporta Series. No 119, IAEA, Viena.

Gustafson A, Ekberg. 1979. Type of mutation. In: manual on Mutation Breeding, Technical Report Series. No. 119, JAEA. Viena.

Hemon, A F. 2006. Efektivitas seleksi in vitro berulang untuk mendapatkan plasma nutfah kacang tanah toleran cekanian kekeringan dan resisten terhadap penyakit busuk batang Scierotium rofsii. Disertasi Doktor Sekolah Pascasarjana IPB, Bogor.

Maluszynski M, Ahlowalia B. S., Sigurbjornsson, B. 1995. Application of in vivo and in vitro mutation techniques for crop improvement. Euphytica 85 : 303-315.

Mexal J. Fisher iT, Osteryoung J. Patric RCP 1975. Oxygen availability in polyethylene glycol solution and its implications in plant-water relation. Plant Physiol. 55: 20-24.

Micke A and Donini B. 1993. Induce mutation. In: Plant Breeding Principle and Prospects (Eds. MD Hasyward, NO Bosemark and I Romagosa). Chapmant and Hall London.

Murashige T. and F. Skoog. 1962. A revised media for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant 15:473-493.

Mohr. H, Schopfer. 1995. Plant Physiology. Springer-Verlag. Berlin.

Pattee H. E. and Stalker H. T. 1995. Advances in peanut science. American Peanut Research and Education Society, Inc. Stiliwater, USA.

Rahayu E. S., E. Guhardja, S. Ilyas, dan Sudarsono. 2005. Seleksi in vitro embrio somatik kacang tanah pada media dengan polietileria glikol untuk mensimulasikan cekaman kekeringan. Majalah llmiah Biologi BIOSFERA.

Van Harten A. V. 1998. Mutation Breeding. Theory and Practical Application. Cambridge University Press. London.


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