EFEKTIVITAS ISOLAT-ISOLAT MIKROB RIZOSFER TERHADAP PERTUMBUHAN SEMAI Paraserianthes falcataria DAN Enterolobium cyclocarpum DI TAILING YANG TERKONTAMINASI MERKURI

Hanna Artuti Ekamawanti, Wiwik Ekyastuti

Abstract


Utilization of rhizosphere microbes such as arbuscular mycorrhizal fungi (AMF) and mercury-resistant bacteria (MRB) is one of the alternative technologies that can be used synergisti-cally to overcome the main problem in biologically rehabilitation of marginal and mercury (Hg) contaminated tailings in ex gold mining area. The research was aimed to get an effective combination of AMF isolates (Glomus sp. SS11, Glomus sp.SS15, Glomus sp.SS18) with MRB isolates (Bacillus sp. HgTA1 and Pseudomonas HgRA) in supporting seedlings growth of sengon laut (Paraserianthes falcataria (L.) Nielsen) and sengon buto (Enterolo-bium cyclocarpum Griseb) in mercury-contaminated tailings. Dual inoculation of Glomus sp. SS15 with Bacillus sp. HgTA1 or Pseudomonas sp. HgRA isolates; dual inoculation of Glomus sp. SS18 with Bacillus sp. HgTA1 or Pseudomonas sp. HgRA isolates; single inoculation or dual inoculation of Glomus sp. SS11 with Pseudomonas sp. HgRA isolates were proven to be very effective in increasing P. falcataria seedlings height. However, only single inoculation of Bacillus sp. HgTA1 isolate was effective in increasing E. cyclocarpum seedlings height. Hg accumulation in plant tissue of P. falcataria and E. cyclocarpum, either with or without rhizosphere microbial inoculation, indicated that both plants were highly potential as Hg phytoextraction or phytostabilization agents.

 

Key words: arbuscular mycorrhizal fungi, Enterolobium cyclocarpum Griseb, mercury, mercury-resistant

bacteria, Paraserianthes falcataria (L.) Nielsen, tailings

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References


Andrea MA, N. Cimento, E.C. Souza. 2003. Operon mer: Bacterial resistance to mercury and poten-tial for bioremediation of contaminated environ-ments; http://funpecrp.com.br/gmr/year2003/ vol1-2/pdf/sim0005.pdf

Astiani, D., H.A. Ekamawanti, E. Susanti. 2001. Studi keanekaragaman cendawan mikoriza arbuskula (CMA) pada tumbuhan pionir di areal bekas per-tambangan emas kawasan hutan alam Mandor kabupaten Landak. Laporan Penelitian. Fakultas Kehutanan Universitas Tanjungpura. Pontianak.

Astiani, D. and H.A. Ekamawanti. 2002. Studi potensi inokulum fungi Glomalean lokal dari areal bekas pertambangan emas rakyat. Laporan Penelitian. Fakultas Kehutanan Universitas Tanjungpura. Pontianak.

Bever. 2002. Host-specificity of AM fungal popula-tion growth rates can generate feedback on plant growth. Plant and Soil 244 (1-2):281-290.

Canstein, H.V., Y. Li, J. Leonhauser, E. Haase, A. Feiske, W.D. Deckwer, I.W. Dobler. 2002. Spa-tially oscillating activity and microbial succes-sion of mercury-reducing biofilms in a technical-scale bioremediation system. Appl. Environ. Microbiol. 68: 1938-1946.

Dang, H., Z.H. Ye, M.H. Wong. 2004. Accumula-tion of lead, zinc, copper and cadmium by wetland plant species thriving in metal-contaminated sites in China. Environ Pollut. 132: 29-40.

Diaz, G., C. Azcon-Aguilar and M. Honrubia. 1996. Influence of arbuscular mycorrhizae on heavy metal (Zn and Pb) uptake and growth of Lygeum spartum and Anthyllis cytisoides. Plant and Soil 180: 241-249.

Ekamawanti, H.A. and D. Astiani. 2005. Uji kes-esuaian fungi Glomalean lokal dari areal bekas pertambangan emas dengan beberapa jenis tana-man inang untuk perbanyakan inokulum. Lapo-ran Akhir Penelitian Dosen Muda. Fakultas Ke-hutanan Universitas Tanjungpura. Pontianak.

Ekamawanti, H.A., R.S. Utomo, Liwono. 2005. Restorasi terestrial, riparian, dan perairan areal bekas penambangan emas dengan teknologi bio-remediasi di kecamatan Mandor kabupaten Lan-dak Kalimantan Barat. Laporan Akhir Penelitian Kegiatan Insentif Riset Matematika dan Ilmu Pengetahuan Alam Fakultas Kehutanan Univer-sitas Tanjungpura. Pontianak.

Ekamawanti, H.A., Liwono, D. Wahyuasti. 2006. Restorasi terestrial, riparian, dan perairan areal bekas penambangan emas dengan teknologi bio-remediasi di kecamatan Mandor kabupaten Lan-dak Kalimantan Barat (Tahun II). Laporan Akhir Penelitian Kegiatan Insentif Riset Matematika dan Ilmu Pengetahuan Alam Fakultas Kehutanan Universitas Tanjungpura. Pontianak.

Ekamawanti, H.A. and D. Astiani. 2007. Produksi inokulum mikoriza arbuskula indigen dari areal bekas penambangan emas dengan beberapa jenis tanaman inang. Makalah dalam Seminar Nasi-onal Mikoriza pada Kongres Mikoriza Indonesia II. Bogor, Jawa Barat.

Ekyastuti, W., T.R. Setyawati, Rafdinal. 2004. Pe-manfaatan isolat bakteri rhizosfer untuk mem-perbaiki kualitas lahan bekas penambangan emas (tailing) di kecamatan Mandor kabupaten Pontianak dalam usaha reklamasi secara biologi. Laporan akhir Hasil Penelitian Hibah Bersaing X. Fakultas Kehutanan Universitas Tanjungpura. Pontianak.

Ekyastuti, W. and H. Artuti. 2007. Isolasi dan efek-tivitas in vitro bakteri resisten merkuri lokal sebagai agen bioremediasi lahan bekas penam-bangan emas. Makalah dalam Seminar Nasional Pertemuan Ilmiah Tahunan Perhimpunan Mik-robiologi Indonesia. Banjarmasin, Kalimantan Selatan.

Esteban, E, E. Moreno, J. Peňalosa, J.I. Cabrero, R. Millán, P. Zornoza. 2008. Short and long-term uptake of Hg in white lupin plants: kinetics and stress indicators. Environ Exp Bot 62:316–322.

Garbisu, C., J. Hernandez-Allica, O. Barrutia, I. Alkorta, J.M. Becerril. 2002. Phytoremedia-tion: a technology using green plants to remove contaminants from polluted areas. Rev. Environ Health 17(3):173-188.

Huang Y., Y. Chen and S. Tao. 2000. Effect of rhi-zospheric environment of VA-mycorrhizal plants on forms of Cu, Zn, Pb and Cd in polluted soil. Ying Yong Sheng Tai Xue Bao 11(3): 431-434.

Koske, R.E. and J.N. Gemma. 1989. A modified pro-cedure for staining roots to detect VA mycorrhi-zas. Mycol. Res. 92 (4):486-505.

McGonigle, T.P., M.H. Miller, D.G. Evans, G.L. Fair-child, J.A. Swan. 1990. A new method which gives objective measure of collonisation of roots by vesicular-arbuscular mycorhirrizal fungi. New Phytol. 115:495-501.

McIntyre, T. 2003. Phytoremediation of heavy met-als from soils. Adv. Biochem. Eng. Biotechnol. 78:97-123.

Meagher, R.B. and A.C.P. Heaton. 2005. Strategies for the engineered phytoremediation of toxic ele-ment pollution: Mercury and arsenic. J. Ind. Mi-crobiol Biotechnol. 32: 502–513

Nakamura, S.K.M., F. Uchiyama, O. Yagi. 1990. Organomercurial-Volatilizing Bacteria in The Mercury-Polluted Sediment of Minamata Bay, Japan. Appl. Environ. Microbial 50:304-305.

Rabie, G.H. 2005. Contribution of arbuscular mycor-rhizal fungus to red kidney and wheat plants tol-erance grown in heavy metal-polluted soil. Afri-can Journal of Biotechnology 4(4): 332-345.

Sieverding, E. 1991. Vesicular-Arbuscular Mycor-rhiza Management in Tropical Agrosystems.Deutsche Gesellschaft fur Technische Zusam-menarbeit (GTZ) GmbH, Eachborn.

Stoltz, E, and M. Greger M. Accumulation properties of As, Cd, Cu , Pb and Zn by four wetland plant species growing on submerged mine tail-ings. Environ. Exp. Bot. 47: 271-280.

Wang, Y. and M. Greger. 2004. Clonal differences in mercury tolerance, accumulation, and distribu-tion in willow. J. Environ. Qual. 33:1779–1785.

Widiastuti, T. and D. Astiani. 2001. Studi karakteristik tanah bekas pertambangan emas rakyat dan suksesi vegetasinya di sekitar hutan kerangas kecamatan Mandor kabupaten Landak. Laporan Penelitian Dosen Muda DIKTI. Pontianak.

Yu, Y., S. Zhang, H. Huang. 2010. Behavior of mercury in a soil–plant system as affected by inocu-lation with the arbuscular mycorrhizal fungus Glomus mosseae. Mycorrhiza 20:407–414.


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