IDENTIFIKASI JAMUR YANG TERBAWA BIJI EMPAT KLON KAKAO (Theobroma cacao L.) PADA PENANGANAN ASALAN DAN PENANGANAN TERKENDALI
DOI:
https://doi.org/10.23960/ja.v24i1.7875
Abstract View: 126
Abstract
Identification is an important activity in the management of plant diseases because by knowing the identity of the fungus found in cocoa beans, a strategy can be determined as a first step before controlling the fungus to prevent the spread of the fungus. This research aims to determine the fungi associated with the MCC 01, MCC 02, Sulawesi 1, and THR clones on uncontrol handling and controlled handling cocoa beans and to determine whether uncontrol handling and controlled handling reduce inoculum sources to suppress fungi growth and development. This research was conducted at the Laboratory of Plant Diseases, Faculty of Agriculture, University of Lampung from January 2023 to March 2023. This research was carried out by testing the agar method (agar plate), the media used was potato sucrose agar (PSA). There were four samples of cocoa bean clones, namely MCC 01, MCC 02, Sulawesi 1, and THR from Kuta Dalom Village, Pesawaran Regency, which were grown on 128 plates of PSA media (64 plates using 2% NaOCl and 64 plates without NaOCl) for each clone. Cocoa beans contained 32 cups each uncontrol handling and controlled handling. So there are 128 cups of PSA media. Isolates that have been obtained are then identified 7 days after isolation. Observations in this study were carried out macroscopically and microscopically. Macroscopic observations were carried out to differentiate growing fungi based on the color, shape and size of the colonies. Meanwhile, microscopic observations were made to differentiate growing fungus based on the structure of the fungi body. The results showed that from the uncontrol handling and the controlled handling, three fungi were found, namely Aspergillus flavus, Aspergillus niger, and Rhizoctonia sp. The fungi Aspergillus flavus and Aspergillus niger show that controlled handling can reduce the source of inoculum compared to uncontrol handling. In contrast to the fungi Aspergillus flavus and Aspergillus niger, the fungi Rhizoctonia sp. shows that controlled handling cannot reduce the source of inoculum.
Key words: Aspergillus, bean, cocoa, Rhizoctonia, handlingDownloads
References
Asrul. (2009). Populasi Jamur Mikotoksigonik dan Kandungan Aflatoksin pada Beberapa Contoh Biji Kakao (Theobroma cacao L.) Asal Sulawesi Tengah. Agroland, 16 (3), 258 – 267.
Badan Pusat Statistik. 2022. Badan Pusat Statistik Provinsi Lampung. https://lampung.bps.go.id./indicator/54/258/1/produksi-tanaman.html. Diakses tanggal 15 September 2023.
Barnett, H. L. 1962. Illustrated Genera of Imperfect Fungi (Second Edition). Burgess Publishing Company. Minneapolis, Minnesota. 225 p.
Campbell, C. K., Jhonson, E. M., & Warnock, D. 2013. Identification of Pathogenic Fungi, (Second Edi). Wiley Blackwell. Hoboken. 337 pp
Direktorat Jenderal Perkebunan. 2015. Statistik Perkebunan Indonesia Komoditas Kakao 2014 – 2016. Direktorat Jenderal Perkebunan, Jakarta. Halaman 7.
El Abbasi, I. H., Khalil, A. A., Awad, H. M., dan Shoala, T. 2020. Nano-diagnostic technique for detection of rice pathogenic fungus Pyriculariaoryzae. Journal Indian Phytopathology, 73(4), 673-682.
Evizal, R. dan Prasmatiwi, F.E. (2023). Struktur Agroforestri Kakao Muda dan Penerimaan Petani di Desa Sidomulyo Kecamatan Air Naningan, Tanggamus. Jurnal Agrotropika, 22(2), 72-83.
Handajani, N.S., & Setyaningsih, R. (2006). Identifikasi jamur dan deteksi aflatoksin B1 terhadap petis udang komersial. Biodiversitas, 7 (3), 212-215.
Liswarni, Y., Nurbailis, & Busniah, M. (2018). Eksplorasi Cendawan Endofit dan Potensinya untuk Pengendalian Phytophthora palmivora Penyebab Penyakit Busuk Buah Kakao. Pros Sem Nas Masy Biodiv Indon, 4(2), 231-235.
Pratama, P.I., Sulistyowati, L., Djauhari, S. (2017). Eksplorasi Jamur Endofit Pada Tanaman Kakao (Theobroma cacao L.) Serta Potensi Antagonismenya Terhadap Phytophthora palmivora Butler Penyebab Penyakit Busuk Buah Secara In Vitro. Jurnal HPT, 5 (2), 61-66.
Pratiwi, N.H., Juliantari, E., & Napsiyah, L.K. (2016). Identifikasi Jamur Penyebab Penyakit Pascapanen pada Beberapa Komoditas Bahan Pangan. Jurnal Riau Biologis, 1, 86-94.
Samson, R. A. 2019. Training Cours 2019 for the Identification of Aspergillus and Fusarium. Fungal Biodiversity Institute. Netherlands.
Sumartini. 2011. Penyakit tular tanah (Sclerotium rolfsii dan Rhizoctonia solani) pada tanaman kacang-kacangan dan umbi-umbian serta cara pengendaliannya. Jurnal Litbang Pertanian, 31(1), 27– 34.
Syatrawati dan Inderiati, S. 2018. Identifikasi Cendawan Pada Biji Kakao Kering Ditingkat Petani. Agroplantae, 7 (2), 8-13.
Wati, E., Hardila, D, W., Raharjo, N, K., dan Sardi, A. 2021. Identifikasi cendawan pada biji kacang (Vigna radiata L.) hijau dengan menggunakan metode blotter test. Journal of Biological Sciences and Applied Biology, 1 (1), 1-8.
Yuktika, Nurdin, M., Ratih, S. 2014. Inventarisasi jamur dan bakteri yang berasosiasi dengan benih padi (Oryza sativa L.) di Lampung. J. Agrotek Tropika, 2 (3), 453-458.
