IMPROVEMENT OF SELECTED INDUCTION CULTURE MEDIA ON CALLUS INDUCTION IN ANTHER CULTURE OF ANTHURIUM AND A HISTOLOGICAL STUDY ON ITS CALLUS FORMATION

  • B Winarto
    Indonesian Ornamental Crops Research Institute
  • N A Mattjik
    Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agriculture
  • A Purwito
    Agronomy and Horticulture Department, Faculty of Agriculture, Bogor Agriculture
  • B Marwoto
    Indonesian Ornamental Crops Research Institute
DOI: https://doi.org/10.23960/ja.v14i1.4217
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Abstract

Studi ini telah dilakukan pada laboratorium kultur jaringan, Balai Penelitian Tanaman Hias Pacet – Cianjur sejak bulan Februari hingga Oktober 2008. Tujuan penelitian ini adalah untuk mengoptimalisasi media terseleksi dalam induksi pembentukan kalus, mengungkap asal-usul sel yang membentuk kalus hasil kultur anther dan proses pembentukan tunasnya. Media terseleksi yang diperbaiki adalah 1) MMS-TBN yang mengandung 0,5 mg/l TDZ, 1,0 mg/l BAP dan 0,01 mg/l NAA (Medium Winarto, WM) dan 2) MMS III yang ditambah dengan 1,5 mg/l TDZ, 0,75 mg/l BAP dan 0,02 mg/l NAA (Medium Winarto dan Rachmawati, WRM). Perlakuan perbaikan dilakukan melalui pembuangan dan penambahan 2,4- D pada konsentrasi 0.5 mg/l dan penurunan kekuatan medium dari kekuatan penuh, setengah, seperempat, seperdelapan, seperenambelas dan nol. Percobaan faktorial disusun menggunakan rancangan acak kelompok dengan empat kali ulangan. Hasil studi ini menunjukkan bahwa induksi kalus tertinggi secara jelas ditemukan pada WRM. Medium ini mampu menginduksi potensi tumbuh anther (PGA) hingga 81% dengan persentase regenerasi anther 49% dan 2.7 kalus per ulangan. Peningkatan yang nyata juga dicatat melalui perlakuan reduksi kekuatan medium dengan seperdelapan kekuatan medium WRM sebagai penurunan kekuatan yang terbaik. Perlakuan ini mampu menstimulasi PGA hingga 58% dengan 29% PAR dan 1.8 NCF. Dari studi histologi diketahui bahwa kalus hasil kultur anther berasal dari sel-sel dinding anther. Kapasitas morfogenesis yang tinggi dari sel-sel dinding anther inilah yang menyebabkan tidak adanya efek androgenesis pada sel-sel mikrospora yang ada didalamnya Kata-kata kunci: Perbaikan, media, kalus, anther, histology dan anthurium

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References

Ahmad, Z. 1993. A Study of The Chemical Manipulations Involved in The Clonal Propagation of Pistacia Vera. Thesis. Institute of Chemistry University of the Punjab, Lahore. 235 pages

Altamura, M. M., A. Cersosimo, C. Majoli and M. Crespan. 1992. Histological study of embryogenesis and organogenesis from anthers of Vitis rupcstris du Lot cultured in vitro. Protoplasma 171:134-141

Chen, U.C., F.S. Chueh, C.N. Hsia, M.S. Yeh and H.S. Tsay. 2005. Influence of 2,4-dichlorophenoxyacetic Acid on Leaf Callus Induction, proliferation and Saikosaponin Formation of in vitro Bupleurum kaoi Liu, Chao et Chuang. Crop, Environment & Bioinformatics 2: 39-49

Eeckhaut, T., S. Werbrouck, J. Dendauw, E. van Bockstaele and P. Debergh. 2001. Induction of homozygous Spatiphyllum wallisii genotypes through gynogenesis. Plant Cell, Tissue and Organ Culture. 67: 181-189.

Han, D.S., Y. Niimi and M. Nakano. 1997. Regeneration of haploid plants from anther cultures of the Asiatic Irbid lily ‘Connecticut King’. Plant Cell, Tissue and Organ Culture. 47: 153-158.

Herman. 2000. Control of Browning during Explant Establishment. Agricell Report. [email protected] . Access date: 6 November 2008

Hoque, A. and S. Arima. 2002 .Overcoming Phenolic Accumulation during Callus Induction and In Vitro Organogenesis in Water Chestnut (Trapa Japonica Flerov). In Vitro Cell. Dev. Biol.—Plant 38:342–346.

Hu, J. B., J. Liu, H. B. Yan and C. H. Xie. 2005. Histological observations of morphogenesis in petiole derived callus of Amorphophallus rivieri Durieu in vitro Plant Cell Rep 24: 642–648EL

Jabeen, N., A.S. Shawl, G.H. Dar, A. Jan and P. Sultan. 2006. Callus induction and organogenesis from explants of Avonitum heterophyllum-medicinal plant. Biotechnology 5(3): 287-291.

Kumar, S and J.K. Kanwar. 2007. Plant Regeneration from Cell Suspensions in Gerbera jamesonii Bolus. Journal of Fruit and Ornamental Plant Research 15: 157-166

Lin, Y.H., C. Chang and W.C. Chang 2000. Plant regeneration from callus culture of a Paphiopedilum hybrid. Plant Cell, Tissue and Organ Culture 62(1): 21-25

Mandal, N. and S. Gupta. 1996. Studies on histomorphological course of events during androgenesis of rice (Oryza sativa L.) Phytomorphology 46(2): 99-107

Maeda, E., V.M. Villalobos and T. Sugiura. 1978. Fine structure of the regenerating cells in Fragaria anther cultures. Japan. J. Breed. 28(2): 143-146.

Mello, M.O., M. Melo and B. Appezzato-da-Glória. 2001. Histological analysis of the callogenesis and organogenesis from root segments of Curcuma zedoaria Roscoe. Brazilian Archives of Biology and Technology. 44(2): 197 – 203.

Mishiba, K., T. Okamoto and M. Mii. 2001. Increasing ploidy level in cell suspension cultures of Doritaenopsis by exogenous application of 2,4-dichlorophenoxyacetic acid. Physiologia Plantarum 112(1): 142 - 148

Mohan Jain, S. and N. Bhalla-Sarin. 1996. Haploidy in Petunia. In: In Vitro Haploid Production in Higher Plants. Mohan Jain, S., S.K. Sopory and R.E. Veileux (Eds.) Volume 5. p: 53-71. Kluwer Academic Publishers. Dordrecht/Boston/London.

Nakano, H and E. Maeda.1989. Cyto histological studies on callus formation and its regeneration in anther culture of Oryza sativa L. Japan. Jour. Crop Sci. 58(3) : 409—418

Nicuta, N.D., I.N. Toma and G.I. Ghiorghi. 2005. Morphogenetical and histological studies of „In Vitro” anther cultures of Brassica Oleracea L. Genetică şi Biologie Moleculară. TOM V: 111-118.

Oggema, J.N., M.G. Kinyua and J.P. Ouma. 2007. Optimum 2,4-D concentration suitable for embryogenic callus induction in local Kenyan sweet potato cultivars. Sian Journal of Plant Sciences 6 (3): 484-489.

Pedroso, M.C. and M.S. Pais. 1996. Anther and microspore culture in Camellia japonica. In: In Vitro Haploid Production in Higher Plants. Mohan Jain, S., S.K. Sopory and R.E. Veileux (Eds.) Volume 5. p: 89-108. Kluwer Academic Publishers. Dordrecht/Boston/London.

Rachmawati, F., B. Winarto and A. Purwito. 2005. Kultur Anther pada Anthurium (Anthurium andreanum Linden ex André). Thesis. Departemen Agronomi and Hortikultura. Fakultas Pertanian. Institut Pertanian Bogor. 146 Halaman.

Raghavan, V. 2004. Role of 2,4-dichlorophenoxyacetic acid (2,4-d) in somatic embryogenesis on cultured zygotic embryos of arabidopsis: cell expansion, cell cycling, and morphogenesis during continuous exposure of embryos to 2,4-d. American Journal of Botany 91(11): 1743–1756.

L.R. Rodrigues, L.R., B. de C. Forte, J.M.S. Oliveira, J.E.A. Mariath and M.H. Bodanese-Zanettini. 2004. Effects of light conditions and 2,4-D concentration in soybean anther culture. Plant Growth Regulation 44: 125–131

Saji, K.V. and M. Sujatha. 1998. Embryogenesis and plant regeneration in anther culture of sunflower (Helianthus anuus L.) Euphytica 103: 1-7.

Svetleva, D.L., and P. Crino. 2005. Effect of ethyl methanesulfonate (ems) and n-nitrose-n.-ethyl urea (enu) on callus growth of common bean. Journal of Central European Agriculture. 6(1): 59-64.

Tanaka, I. and M. Ito. 1981. Studies on microspore development in Liliceous Plant III. Pollen tube development in Lily pollens cultured from the uninucleate microspore stage. Plant and Cell Physiology. 22(1):149-153.

__________________. 1982. Additional mitosis induced by bromodeoxyuridine in explanted tulip microspores. Plant Science Letters. 27: 37-42.

Thengane, S.R., M.S. Joshi, S.S. Khuspe, and A. E Mascarenhas. 1994. Anther culture in Helianthus annuus L., influence of genotype and culture conditions on embryo induction and plant regeneration. Plant Cell Reports 13:222-226

Van den Bulk. R.W., H.P.J. de Vries-van Hulten, J.B.M. Custers and J.J.M. Dons. 1994. Induction of embryogenesis in isolated microspores of tulip. Plant Science. 104: 101-111.

Wang, J. and M.Z. Bao. 2007. Plant regeneration of pansy (Viola wittrockiana) ‘Caidie’ via petiole-derived callus. Scientia Horticulturae. 111(3): 266-270

Winarto, B dan F. Rachmawati. 2007. Teknik kultur anther pada pemuliaan anthurium. J. Hort. 17(2): 127-137.

Winarto, B., F. Rachmawati, N.A. Mattjik, A. Purwito, dan B. Marwoto. 2009a. Pengembangan formulasi medium dasar untuk kultur anther anthurium. Dikirim untuk dipublikasikan pada Jurnal Stigma Fakultas Pertanian, Universitas Andalas, Padang, pada tanggal 20 April 2009.

B. Winarto, F. Rachmawati dan D. Pramanik. 2009b. Pengaruh cara isolasi, jenis dan konsentrasi agar terhadap induksi pembentukan kalus pada kultur anther anthurium. Dikirim untuk dipublikasikan pada Jurnal Hortikultura, Pusat Penelitian dan Pengembangan Hortikultura Jakarta, pada tanggal 23 Januari 2009.

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How to Cite
Winarto, B., Mattjik, N. A., Purwito, A., & Marwoto, B. (2020). IMPROVEMENT OF SELECTED INDUCTION CULTURE MEDIA ON CALLUS INDUCTION IN ANTHER CULTURE OF ANTHURIUM AND A HISTOLOGICAL STUDY ON ITS CALLUS FORMATION. JURNAL AGROTROPIKA, 14(1). https://doi.org/10.23960/ja.v14i1.4217