PERTUMBUHAN DAN PERBANYAKAN TUNAS MIKRO SINGKONG (Manihot esculenta CRANTZ.) SECARA IN VITRO PADA BERBAGAI KONSENTRASI BENZIL ADENIN.
Abstract
Propagation of cassava through in vitro culture is required by farmers and agro industries to fulfill the need of the best and the newest clone as soon as after that clone is released by government. The objective of this research is to know the effect of the application of four concentration of benzyl adenine on the growth and proliferation of cassava shoot in vitro. Explants used were one-node cuttings of cassava about 1 cm length, grown in polybags. This research was arranged in completely randomized design with the treatments consisting of four concentrations of benzyl adenine: 0.5, 1, 2 and 4 mg/l added of basic media of Murashige and Skoog. Each treatment was replicated 4 times with 5 explants in each experimet al unit. The best growth and micro-shoot propagation of cassava in vitro was indicated by treatment of 0.5 mg/l benzyl adenine.
Key words: cassava, benzyl adenin, in vitro, micro-shoot
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Arous, S., M. Boussaid and M. Marrakchi. 2001. Plant regeneration from zygotic embryo hypocotyls of Tunisian chili (Capsicum annum L.). J Appl. Hort. 3(1): 17-22.
Badan Pusat Statistik Lampung. 2006. Lampung Dalam Angka 2006. BPS Lampung dan Bappeda Propinsi Lampung. 622 hlm.
Balai Informasi Pertanian Irian Jaya. 1995. Budidaya Singkong (Manihot esculenta Cranzt.). Lembar Informasi Pertanian, BIP Irian Jaya 150/95. http://www.pustaka-deptan.go.id.
Esti dan K. Prihatman. 2000. Tepung tapioca. http://bebas.vlsm.org/v12/artikel/pangan.
Food and Agriculture Organization. 2007. Cassava. http://www.fao.org.
Geetha, C. K., P. A. Nazeem, and L. S. Joseph. 1990. In vitro callus induction in Black Pepper. Indian Cocoa, Arecanut and Spices Journal 14(1):34-36.
George, F. E. and P. D. Sherington. 1984. Plant Propagation by Tissue Culture. Exegetics Ltd. England.
Gopi, C., Y. N. Sekhar, and P. Ponmurugan. 2006. In vitro multiplication of Oncimum gratissimum L. Through direct regeneration. Af. J. Biotech. 5(9): 723-726.
Haw, A. B. and C. L. Keng. 2003. Micropropagation of Spilanthes acmella, a bio-insecticide plant, through proliferation of multiple shoots. J. Appl. Hort. 5(2): 65-68.
Ko, C. Y., J. P. Kung, and R. Mc Donald. 2008. In vitro micropropagation of white dasheen (Colocasia esculenta). Af. J. Biotech. 7(1): 41-43.
Konan, N. K., C. Schopke, R. Carcamo, R. N. Beachy, and C. Fauquet. 1997. An efficient mass propagation system for cassava (Manihot esculenta Crantz) based on nodal explants and axillary bud-derived meristems. Plant Cell Rep. 16: 444-449.
Mok, M. C., S. G. Kim, D. J. Amstrong, and D. W. S. Mok. 1979. Induction of cytokinin autonomy by N`,N`-diphenylurea tissue cultures of Phaseolus lunatus L. Proc. Natl. Acad. Sci. USA 76: 3880-3884.
Ndiaye, A., M. S. Dialo, D. Niang and Y. K. Gassama-Dia. 2006. In vitro regeneration of adult trees of Bambusa vulgaris. Af. J. Biotech. 5(13): 1245-1248.
Ndoye, M., I. Diallo and Y. K. Gassama-Dia. 2003. In vitro multiplication of the semi-arid forest tree, Balanites aegyptiaca (L.) Del. Af. J. Biotech. 2(11): 421-424.
Pierik, R. L. M. 1987. In Vitro Culture of Higher Plants. Martin Nijhoff Publ. Netherland. p. 344.
Poornima, G. N. and R. Rai. V. 2007. In vitro propagation of wild yam, Dioscorea oppositifolia L. And Dioscorea pentaphylla L. Af. J. Biotech. 6(20): 2348-2352.
Raemakers, K., E. Jacobsen, and R. Visser. 2000. The use of somatic embryogenesis for plant propagation cassava. Mol.Biotech. 14: 215-221.
Rahman, M. A. and J. Blake. 1988. Factors affecting in vitro proliferation and rooting of shoot of Jackfruit (Artocarpus heterophyllus Lam.). Plant Cell Tissue and Organ Culture 13: 179-187.
Stamp, J. A., S. M. Colby, and C. P. Meredith. 1990. Improved shoot organogenesis from leaves of Grape. J. Amer. Soc. Hort. Sci. 115(6):1038-1042.
DOI: http://dx.doi.org/10.23960/ja.v14i1.4225
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