REGENERASI IN VITRO TANAMAN TEBU (Saccharum officinarum L.): INDUKSI DAN PROLIFERASI KALUS, SERTA INDUKSI TUNAS

Ronald Bunga Mayang, Dwi Hapsoro, Yusnita Yusnita

Abstract


This research is a series of activities that studies the embryogenesis for somatic clonal reproduction plant sugarcane multiplication in vitro which in-cludes the callus induction of explant pieces rolls up the leaves, the proliferation of embriogenic callus, and shoots induction of embriogenic callus. Those of three researchs were conducted in a series. This experiment were carried out in The Plant Science Laboratory, the Faculty of Agriculture, the University of Lampung, Indonesia, from Oktober 2010 until April 2011. All experiments were conducted in a completely random-ized design. Treatments of Experiment I were 2,4-D concentrations (0, 1, 2, and 3 mg/l) with and without 250 mg/l of casein hydrolysate, while those of Experiment II were combinations of those two chemicals, 2,4-D concentrations (1, 2, and 3 mg/l) with and without 250 mg/l of casein hydrolysate. Treatments of Experiment III were BA concentrations (1 mg/l and 2.5 mg/l). Results of the experiments showed that the best callus induction was achieved using MS media containing 3 mg/l 2,4-D, and callus induction was not significantly affected by casein hidrolysates. Concentrations of 2,4-D did not significantly influence em-bryogenic callus proliferation. The best shoot proliferation was attained in MS media containing 2.5 mg/l.

 

Keywords: Benzyladenine (BA), 2,4-D, Casein Hydrolisate, sugarcane, and culture in vitro.

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References


Ali, A., S. Naz, F.A. Siddiqui, and J. Iqbal. 2008. Rapid clonal multiplication of sugarcane (Sac-charum officinarum L.) through callogenesis and organogenesis. Pakistan Journal Botany. 4(1): 123—128.

Behera, K.K. and S. Sahoo. 2009. Rapid In vitro mi-cro propagation of sugarcane (Saccharum of-ficinarum L. cv-Nayana) through callus culture. Nature and Science. 7(4): 1-10.

Farid, M. 2003. Perbanyakan tebu (Saccharum of-ficinarum L.) secara in vitro pada berbagai kon-sentrasi IBA dan BAP. J. Sains & Teknologi. 3(30): 103—109.

Febrianie, A. P. 2008. Pengaruh benziladenin (BA) pada pembentukan tunas dari kalus tebu (Sac-charum officinarum L.) secara in vitro melalui organogenesis. Skripsi. Universitas Lampung. Bandar Lampung.

Gamborg, O.L. and G.C. Phillips. 1995. Plant Cell, Tissue and Organ Culture: Fundamental Meth-ods. Springer Lab Manual. Germany.

George, E.F., M.A. Hall, dan G.J.D. Klerk. 2008. Plant Propagation by Tissue Culture 3rd Edi-tion. Springer. Netherlands.

Julian. 2011. Produksi Gula Jalan di Tempat. http:// www.agroindonesia.co.id. Diakses 26 De-sember 2011.

Khan, I.A., and A. Khatri. 2006. Plant regeneration via organogenesis or somatic embryogenesis in sugarcane: Histological studies. Pak. J. Bot. 38(3): 631—636.

Murashige, T. 1974. Plant propagation through tis-sue culture. Ann. Rev. Plant Physiol. 25: 135—166.

Nasir, I.A., G.Z. Jahangir, Z. Qamar, Z. Rahman, and T. Husnain. 2011. Maintaining the regen-eration potential of sugarcane callus for longer span. African Journal of Agricultural Research. 6(1): 113—119.

Nuryani, I. 2006. Tanggapan pertumbuhan nilam (Pogostemon cablin Benth) terhadap jenis me-dia dasar dan penambahan benzyladenin secara in vitro. Skripsi. Universitas Lampung. Ban-dar Lampung.

Rival, A. dan G.K.A. Parveez. 2005. Elaeis guinen-sis Oil Palm. In: R.E. Litz (ed). Biotechnol-ogy of Fruit and Nut Crops. CABI Publishing. USA. p 113—143.

Shaheen, M.S. and M.S. Mirza. 1989. In vitro pro-duction of plants from sugarcane tissue. Jour-nal of Agric. Sci. 26: 302—312.

Siregar, L.A.M., C. Lai-Keng, dan B. Peng-Lim. 2010. Pengaruh kasein hidrolisat dan intensitas cahaya terhadap produksi biomassa dan alka-loid canthinone di dalam kultur suspensi sel pa-sak bumi (Eurycoma longifolia Jack). Makara Sains. 14(1): 12—21.

Supriyatdi, Dedi. 2010. Respons Eksplan Tebu (Sac-charum officinarum L.) Terhadap 2,4-D dan Kasein Hidrolisat Dalam Proses Induksi Kalus, Induksi Tunas, dan Pengakaran In Vitro. Tesis. Universitas Lampung. Bandar Lampung.

Suryana, A., D. H. Goenadi, W. R. Susila, Nahdo-din, dan H. Malihan. 2005. Prospek dan Arah Pengembangan Agribisnis Tebu. Badan Peneli-tian dan Pengembangan Pertanian Departemen Pertanian.

Trigiano, R.N. and D.J. Gray. 2010. Plant Tissue

Culture, Development, and Biotechnology.

CRC Press. Florida.

Yakub, M. 2011. Pabrik Gula Berkapasitas 10 Ribu Ton Dibangun di Lamongan. http: MediaIndo-nesia.com. Diakses pada tanggal 17 Juni 2011.

Yusnita. 2003. Kultur Jaringan Cara Memperbanyak Tanaman Secara Efisien. AgroMedia Pustaka. Jakarta.




DOI: http://dx.doi.org/10.23960/ja.v16i2.4269

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