Wartono Wartono, Yadi Suryadi, Dwi Ningsih Susilowati


In vitro study was carried out to test the effectiveness of the formulation containing Burkholderia cepacia isolates E76 in suppressing growth of Rhizoctonia solani and its effect on plant growth. The study was conducted at Plant Disease Laboratory, Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development Bogor, in January to July 2012. The in vitro method was done by growing the bacteria B. cepacia isolates E76 together with R. solani in one dish of potato dextrose agar (PDA) medium. Seed of “Ciherang” rice was treated with formulation of B. cepacia isolates E76. Observation were done to relative inhibitory rate, rice germination, shoot length, and root length. Results showed that B. cepacia isolates E76 formulation was effective in suppressing the growth of R. solani with relative inhibitory rate at 24 and 48 hour after incubation, respectively 31,3 – 60,2% and 28,9 – 47,8%. Rice germination and growth on treated rice seeds were better than control treatment. Concentration at 3% concentration was suggested to be used as recommended concentration for further testing.


Key words: Formulation, Burkholderia cepacia isolat E76, Rhizoctonia solani

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Bevivino, A., C. Dalmastri, S. Tabacchioni, and L. Chiarini. 2000. Efficacy of Burkholderia cepacia MCI 7 in disease suppression and growth promotion of maize. Biology and Fertility of Soils Journal. 31(3-4):225-231.

Gerhardson, B. 2002. Biological substitutes for pesticides. Trends Biotechnol. 20:338–343.

Hadiwiyono. 1999. Jamur akar gada (Plasmodiphora brassicae Wor.) pada cruciferae: uji toleransi inang dan pengendaliannya secara hayati dengan Trichoderma. Prosiding Kongres Nasional XV dan Seminar Ilmiah Perhimpunan Fitopatologi Indonesia; Purwokerto, 16-18 September 1999. Purwokerto: Universitas Jenderal Soedirman. p. 365-371.

Hernandez, A., I. Fernandez, P. Ana, J. Miranda, F.C. Sandra, N.H. Ana, and J.L. Santander. 1999. Production, purification and diagnosis of siderophores from Pseudomonas fluorescens strain J-1443. Tropical Crops. 20 (1): 21-25.

Mendes, R., A.A. Pizzirani-Kleiner, W.L. Araujo, and J.M. Raaijmakers. 2007. Diversity of cultivated endophytic bacteria from sugarcane: genetic and biochemicl characterization of Burkholderia cepacia complex isolates. Appl. Environ. Microbiol. 73: 7259-7267.

Meyer, J.M., V. Tran, A. Stinzi, O. Berge, and G. Winkelman. 1995. Ornibactin production and transport properties in strains of Burkholderia cepacia and Burkholderia vetnamienses (formely Pseudomonas cepacia). Biometal. 8:309-307.

Salim, W., S. Christanti, dan B. Hadisutrisno. 2003. Pengimbasan ketahanan pisang terhadap penyakit layu fusarium dengan Burkholderia cepacia. Agrosains. 5(2):72-79.

Tran Van V., O. Berge, S.K. Ngo, J. Balandreau, and T. Heulin. 2000. Repeated beneficial effects of rice inoculation with a strain of Burkholderia vietnamiensis on early and late yield components in low fertility sulphate acid soils of Vietnam. Plant Soil. 218: 273-284.

Zaki, K., I.J. Misaghi, A. Heydary, and M.N. Shatla. 1998. Control of Cotton Seedling Damping-off in the Field by Burkholderia (Pseudomonas) cepacia AMMD of Four Pea Cultivars. Plant Disease. 82: 291-193.



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