PENGARUH PERIODE IMBIBISI TERHADAP INDUKSI EMBRIO SOMATIK DUA VARIETAS KACANG TANAH (Arachis hypogea L.) SECARA IN VITRO

Akari Edy; Resmia Fajarwati Hendrady; Setyo Dwi Utomo

doi:10.23960/ja.v18i1.4288

Authors

Akari Edy Jurusan Agroteknologi, Fakultas Pertanian Universitas Lampung
Resmia Fajarwati Hendrady Jurusan Agroteknologi, Fakultas Pertanian Universitas Lampung
Setyo Dwi Utomo Jurusan Agroteknologi, Fakultas Pertanian Universitas Lampung

DOI:

https://doi.org/10.23960/ja.v18i1.4288

Abstract View: 5922

Abstract

The objectives of the experiment were to (1) investigate the effect of seed imbibitions period on inducing somatic embryo of peanut varieties, (2) determine the best imbibitions period. The experiment was arranged in a completely randomized design with eight replicates. Each experimental unit consisted of culture bottles each of which contained five leaflet explants. Two varieties of peanut of var. Kancil and var. Singa were imbibed in water for 0, 20, 40, and 60 hours before sown aseptically on MS medium. Data were subjected to analysis of variance and if there was any significant F result (s), the means were separated using least significant difference at P≤ 0.05. Results of the experiment showed that: (1) Imbibitions period had significant effect on inducing somatic embryo of peanut varieties, (2) For var. Kancil, the best embryo somatic numbers and embryonic callus percentage were produced by 40 hours of imbibitions, while for var. Singa were produced by 20 hours imbibitions.

Key words: peanut, imbibitions period, MS medium, somatic embryo.

Downloads

Download data is not yet available.

References

Dewi, I.R. 2008. Peranan dan Fungsi Fitohormon bagi Pertumbuhan Tanaman. Fakultas Pertanian Universitas Padjajaran. Bandung.

Edy, A. 2008. Pengaruh umur kecambah sumber eksplan leaflet terhadap induksi embrio somatik pada beberapa varietas kacang tanah secara in vitro. Prosiding Seminar Nasional SMAP 2009. Universitas Lampung. Bandar Lampung.

Murashige, T. dan F. Skoog. 1962. A Revised Medium For Rapid Growth dan Bioassay With Tobacco Tissue Cultures. Physiology Plant. 15 : 473 – 479.

Murthy B.N.S. and P.K. Saxena.1994. Somatic embryogenesis in peanut (Arachis hypogaea L): Stimulation of direct differentiation of somatic embryos by forchlorfenuron (CPPU). Plant Cell Report 14:145-150.

Purnamaningsih R. 2003. Regenerasi Tanaman Melalui Embriogenesis Somatik Dan Beberapa Gen Yang Mengendalikannya. Buletin Agrobio 5(2):51-58.