Respons Pertumbuhan Planlet Anggrek Phalaenopsis terhadap Pemberian BA dan NAA Selama Aklimatisasi
DOI:
https://doi.org/10.23960/ja.v22i2.7855
Abstract View: 581
Abstract
Orchid plants are a type of plant that belongs to the Orchidaceae family which has approximately 900 genera and estimated to have 15.000- 25.000 species. Orchid plants can be cultivated vegetative and generative. Tissue culture is one of the vegetative propagation methods of orchids. Acclimatization is a critical period for tissue cultured plantlets. Acclimatization is a critical period for plantlets resulting from tissue culture. Plant growth regulators can help stimulate plantlet growth during the acclimatization period. growth regulators is one of the success factors in acclimatization. This study aims to determine the best BA and NAA concentrations and the best treatment combination for acclimatization of Phalaenopsis orchids. This research was conducted from March to July 2022 at the Research and Experimental station, University of Bangka Belitung. This study used a factorial randomized block design (RBD). The factors tested were the concentrations of BA and NAA solutions. The results showed that the acclimatization of Phalaenopsis orchids was successful, indicated by the high percentage of plantlet survival. The highest average growth was shown at concentrations of 10 ppm BA and 10 ppm NAA. The treatment combination showing the highest average growth was BA 20 + NAA 10 ppm.
Keywords : Acclimatization, BA, NAA, Phalaenopsis
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References
Apriliyania, R., & Wahidah, B. F. 2021. Perbanyakan anggrek Dendrobium sp . secara in vitro : Faktor-faktor keberhasilannya. Filogeni: Jurnal Mahasiswa Biologi, 1(2): 33–46.
Asra, D. R., Samarlina, R. A., & Silalahi M. 2020. Hormon Tumbuhan. UKI Press. Jakarta
Astutik, Sumiati, A., & Sutoyo. 2021. Stimulasi Pertumbuhan Dendrobium sp Menggunakan Hormon Auksin Naphtalena Acetic Acid ( NAA ) dan Indole Butyric Acid ( IBA). Jurnal Buana Sains, 21(1): 19–28.
Bella, D. R. S ., Suminar, E., Nuraini, A., & Ismail, A. 2016. Pengujian Efektivitas Berbagai Jenis Dan Konsentrasi Sitokinin Terhadap Multiplikasi Tunas Mikro Pisang (Musa paradisiaca L.) Secara In Vitro. Jurnal Kultivasi, 15(2): 74–80.
Budiarti, N. 2010. Pengaruh Media dan Cara Tanam terhadap Aklimatisasi dan Pengaruh Benziladenin dan Viamin B1 terhadap Petrumbuhan Anggrek Dendrobium. Skripsi. Universitas Lampung.
Cha-um, S., Science, N., Agency, T. D., Ulziibat, B., & Kirdmanee, C. 2010. Effects Of Temperature And Relative Humidity During In Vitro Acclimatization , On Physiological Changes And Growth Characters of Phalaenopsis Adapted to In Vivo. Australian Journal of Crop Science, 4(9): 750–756.
Dwiyani, R. 2015. Kultur Jaringan Tanaman. Bali: Pelawa Sari.
Dyah, W. (2014). Pengaruh Auksin dan Sitokinin Terhadap Pertumbuhan Planlet Anggrek Mokara. J. Hort, 24(3): 230–238.
Fauziah, N., Aziz, S. A., & Sukma, D. 2014. Karakterisasi Morfologi Anggrek Phalaenopsis spp. Spesies Asli Indonesia. Buletin Agrohorti, 2(1): 86-94.
George, E.F. and Sherrington, P.D. 1984. Plant Propagation by Tissue Culture: A Hand Book and Dictionary of Commercial Laboratories. Exgetics Ltd., England.
Handayani, M. Y. 2011. Persilangan Dialel Lengkap Dua Tetua Anggrek, Pengecambahan Biji Dan Pembesaran In Vitro Serta Aklimatisasi Planlet Phalaenopsis. Tesis. Universitas Lampung.
Hapsoro, D., Febrianie, A. P., & Yusnita. 2012. In Vitro Shoot Formation on Sugarcane (Saccharum officianarum L.) Callus as Affected by Benzyladenine Concentrations. J. Agron. Indonesia, 40(1): 56–61.
Hartati, S., Budiyono, A., & Cahyono, O. 2016. Pengaruh NAA dan BAP Terhadap Pertumbuhan Subkultur Anggrek Hasil Persilangan Dendrobium biggibum X Dendrobium liniale. Caraka Tani :Journal of Sustainable Agriculture, 31(1): 33–37.
Isda, M. N., Fatonah, S., Lestari, W., Hutapea, E. Y., & Purba, L. 2014. Induksi Tunas dan Pembentukan Akar Dari Eksplan Kotiledon Jeruk Siam (Citrus Nobilis LOUR.) Asal Kampar Secara In Vitro. SEMIRATA Bidang MIPA, 1–8.
Isniani, Y., Handayani, I., Novitasari, Y., Febrianto, Y., Erwansyah, D., Rukmanto, H., Setiabudi, M., Asikin, D., & Aprilianti, P. 2021. Aklimatisasi dan Diseminasi Bibit Kantong Semar (Nepenthes spp.) Hasil Kultur Jaringan di Kebun Raya Bogor. Warta Kebun Raya, 19(2): 14–23.
Mastuti, R. 2017. Dasar-Dasar Kultur Jaringan Tumbuhan. UB Press. Malang.
Mirani, A. A., Abul-soad, A. A., & Markhand, G. S. 2017. In Vitro Rooting of Dendrobium nobile Orchid : Multiple Responses to Auxin Combinations. Not Sci Biol, 9(1): 84–88.
Natasaputra, L. 2016. Budidaya Anggrek Phalaenopsis. Sunda Kelapa Pustaka. Jakarta.
Nikmah, Z. C., Slamet, W., & Kristanto, B. A. 2017. Aplikasi Silika Dan NAA Terhadap Pertumbuhan Anggrek Bulan (Phalaenopsis amabilis L .) pada Tahap Aklimatisasi. J. Agro Complex, 1(3): 101–110.
Taiz, L., & Zeiger, E. 2002. Plant Physiology (3 rd). Sunderland: Sinauer Associates.
Yuan, S. C., Chin, S. W., Lee, C. Y., & Chen, F. C. 2018. Phalaenopsis pollinia storage at sub ‑ zero temperature and its pollen viability assessment. Botanical Studies, 1–8.
Zasari M. 2010. Studi Perbanyakan dan Regenerasi In Vitro Protocorm-Like Bodies Serta Aklimatisasi Planlet Anggrek Dendrobium Hibrida. Tesis. Bandar Lampung: Program Pasca Sarjana Magister Agronomi. Universitas Lampung.
Zasari, M., Yusnita, & Susriana. 2014. Respon Pertumbuhan Planlet Anggrek Phalaenopsis Hibrida Terhadap Pemberian Dua Jenis Pupuk Daun dan Benziladenin Selama Aklimatisasi. Enviagro, Jurnal Pertanian Dan Lingkungan, 7(2): 33–38.
