PRODUKSI ANTIBODI UNTUK BAGIAN RIBOSOME KAMIR YANG MAMPU MENGIKAT MIKOTOKSIN TRIKOTESEN [Production of Antibodies Against Trichothecene - Yeast Ribosomal Binding Site)

Abstract

Attempts to develop ELISAs that could be usedto detect "total load" trichothecenes in foods were made by producing antibodies against trichothecene ribosomal binding site. Twelve female BALB/c mice were immunized with either the 60S or 80s ribosomal subunits that were isolated from a trichodermin-sensitive yeast. Another ten female BALB/c mice were injected with the mixture of 80S subunits with different adjuvants. All mice apparently produced high titer antisera. These antisera were incubated simultaneously with trichothecenes over solid-phase bound 80S ribosomal subunits. Because of their specificity, they could not inhibit the association of the toxin and antisera could not be used to detect "total load" trichothecenes in foods.