EFEK KOPIGMENTASI DARI KATEKOL DAN TANIN TERHADAP STABILITAS ANTOSIANIN BEKATUL BERAS KETAN HITAM (Oryza sativa glutinosa) SELAMA PENYIMPANAN [Copigmentation Effect of Catechol and Tannin on Stability of Glutinous Black Rice Bran (Oryza sativa glutinosa) Anthocyanins During Storage]

Dian Wulandari, Tirza Hanum, Azhari Rangga


Copigmentation has been suggested as an effective method to improve color and stability of anthocyanins and storage time.  Catechol and tannin can be used as a copigment to improve stabilize color of anthocyanins. This study aims to examine the effect of molar ratio of copigment to anthocyanin and storage time which stabilize anthocyanin.

This study was arranged in factorial Randomized Complete Block Design (RCBD) with three replications. First factor were molar ratio 0:1, 50:1, 100:1; and second factor were time of storage 0, 10, 20, 30, 40 days at room temperature (28oC ± 2 oC).  Color and stability of anthocyanins were evaluated by thrugh determination of bathochromic and hyperchromic, anthocyanins concentration, retention, and kinetic parameters.  Variables were analyzed by Tuckey test, Bartlet test, and Polynomial Orthogonal test.

The results showed that anthocyanin content of glutinous black rice bran extract was 426 mg/100g. Copigmentation with catechol resulted in bluish color (hypsochromic), while tannin resulted in reddish color (bathochromic).  The best molar ratio of catechol copigment was 50: 1 showed by decreasing of anthocyanin concentration of 0.35mM/day or 39.29mg/100g/day, color retention 12.78%/hour, and half-life time 8.66 hours. The best molar ratio of tannins copigment was 100: 1 showed by decreasing of anthocyanin concentration of 0.07mM/day or 7.86mg/100g/day, color retention was 2.39%/day, and half-life time of 19.80 hours.


Copigmentation; catechol; tannin; anthocyanins; glutinous black rice bran

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Berké, B. and V.A.P. Freitas. 2005. Influence of procyanidin structures on their ability to complex with oenin. Food Chemistry. 90 (3): 453–460.

Boulton, R. 2001. The copigmentation of anthocyanins and its role in the color of red wine: a critical review. Am. J. Enol. Vitic.. 52(2): 67-87.

Castaneda-Ovando A., M.L. Pacheco-Hernandez, M.E. Paez-Hemandez, J.A. Rodrigue and C.A. Galan-Vidal. 2009. Chemical studies of anthocyanins: a review. Food Chemistry. 133: 859-971.

Darias-Martin, J., M.C. Lopez, J.F.E. Granado and C.D. Romero. 2007. The Magnitude of Copigmentation In The Colour of Aged Red Wines Made in the Canary Islands. European Food Research and Technology, 224(5):643-648.

Francis, F.J. and I.A. Bassa. 1987. Stability of Anthocyanin from Sweet Potatoes in a Model Beverages. Journal of Food Science. 52 (6) : 1753-1754.

Gao, K. and G. Mazza. 1996. Extraction on Anthocyanin Pigments from Purple Sun Flower Hulls. Journal of Food Science. 61(3): 600-603.

Gauce C., E.S. Malagoli, and M.T.B. Luiz. 2010. Effect of ph on the copigmentation of anthocyanins from carbernet sauvigon grape extracts with organic acid. Science Agricuture (Piracicaba, Braz.), 67 (1):41-46.

Giusti, M. M., and Wrolstad, R. E. 2001. Anthocyanins. Characterization and measurement with uv-visible spectroscopy. InR.E. Wrolstad (Ed.). Current Protocols in Food Analytical Chemistry. New York :Wiley.

Jackman, R.L. and J.L Smith. 1996. Anthocyanin and betalains. Natural Food Colourants. Second Edition. Blackie Academic and Profersionals. London.

Kalisz, S., J. Oszmianski, J. Hladyszowski and M. Mitek. 2013. Stabilization of anthocyanin and skullcap flavone complexes – investigations with computer simulation and experimental methods. Polandia. Food Chemistry, 138(1):491–500.

Khaldun, I.. 2013. Kestabilan zat warna alami dari umbi ketela ungu (Ipomoea batatas). J Chimica Didactica Acta. 1 (1):34-40.

Kopjar, M. and V. Pilizota. 2009. Copigmentation effect of phenolic compounds on red currant juice anthocyanins during storage. J. Food Sci Technol. 1(2):16-20.

Mahkamah, S. 2004. Perbandingan Stabilitas Panas Ekstrak Antosianin Bekatul Beras Ketan Hitam (Oryza sativa glutinosa) dan Tanaman Hati Ungu (Tradescantia pallida). Skripsi. Universitas Lampung. Lampung.

Markovic, J.M.D., N.A. Petranovic, and J.M. Baranac. 2000. A Spectrophotometric Study of the Copigmentation of Malvin with Caffeicand Ferulic Acids. J Agric Food Chem, 48:5530-5536.

Mastuti, E., G. Fristianingrum, dan Y. Andika. 2013. Ekstraksi dan Uji Kestabilan Warna Pigmen Antosianin dari Bunga Telang (Clitoria ternatea L.) sebagai Bahan Pewarna Makanan. Simposium Nasional RAPI XII. ISSN 1412-9612. Universitas Sebelas Maret.

Mateus N., and V. Freitas.2008. Anthocyanins as Food Colorants. In: Winefield C., Davies K., Gould K. (eds) Anthocyanins. Springer, New York, NY. Pp 284 – 304.

Mazza G. and R. Brouillard. 1990. The Mechanism of Copigmentation of Anthocyanins in Aqueous Solutions. Phytochemistry, 29:1097-1102.

Pebrianti, C., R.B. Ainurrasyi dan S.L. Purnamaningsih. 2015. Uji kadar antosianin dan hasil enam varietas tanaman bayam merah. Jurnal Produksi Tanaman 3(1): 27-33.

Palamides, N. and P. Markakis., 1978. Stability of Grape Anthocyanin in Carbonated Beverages. Semana Vitivicola 33: 2633-2639.

Rein, M.J. dan M. Heinonen. 2004. Stability and Enhancement of Berry Juice Color. J. Agric. Food Chemistry. 52 (25):3106-3114

Rein, M.J. 2005. Copigmentation Reactions and Color Stability of Berry Anthocyanins. Doctoral Manuscript. University of Helsinki Department of Applied Chemistry and Microbiology Food Chemistry Division. Helsinki. 87 pp.

Setyadi, R. 2014. Penentuan Kandungan Total Antosianin pada Berbagai Bunga dengan Metoda Spektrofotometri pH Differential. Thesis. Universitas Andalas. Padang.

Scheffeldt, P. and G. Hrazdina, 1978. Co-pigmentation of anthocyanins under physiological conditions. Journal of Food Science. 43 (2):517-520.

Shao Y., F. Xu, X. Sun, J. Bao, and T. Beta. 2014. Identification and quantification of phenolic acid and anthocyanins as antioxidants in bran, embryo, and endosperm of whine, red, and black rice kernels (Oryza sativa L). Journal Cereal Science. 59:211-218.

Shi, Z. and F.J. Francis, 1992. Anthocyanins of tradescantiapallida: potential food colorants. Journal of Food Science. 57(3): 761-765.

Steel R.G.D. and J.H. Torrie. 1991. Prinsip dan Prosedur Statistika. PT. Gramedia. Jakarta.

Talcott ST, C.H. Brenes, D.M. Pires and D.D. Pozo-Insfran. 2003. Phytochemical stability and color retention of copigmented and processed muscadine grape juice. J Agric Food Chem. 51(4): 957-963

Wilska-Jeszka J and A. Korzuchowska. 1996. Anthocyanins and Chlorogenic Acid Copigmentation. Influence on the Color of Strawberry and Chokeberry Juices. Lebensm Unters Forsch. 203(1):38-42.


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