Effect of Thawing Process on the Quality of Chicken Thigh Meat
Abstract
Frozen meat handling, especially during the thawing stage, can affect meat quality and influence the final product. This study aimed to evaluate the effects of thawing method and duration on the quality of frozen and steamed chicken thigh meat. The experiment was arranged in a completely randomized design with two replications. The thawing methods included room temperature (4, 5, 6 h), blast thawing (75, 105, 135 min), and cold temperature (18, 21, 24 h). The observations included drip loss, total plate count (TPC), total free fatty acids (FFA), and soluble protein content. The effect of thawing on steamed meat quality was evaluated for the texture, protein content, fat content, and hedonic scores. The data were statistically analyzed using one-way ANOVA test continued by independent sample t-test. The results showed that thawing duration within the same method did not significantly affect drip loss or TPC, but longer thawing times increased FFA across all methods. Prolonged thawing at room and cold temperature significantly reduced soluble protein content. Thawing at cold temperature for 18 h was the most effective with the lowest drip loss (1.12%) and the highest soluble protein content (34.4 mg/g). Results of steamed meat analysis showed significant differences in texture and fat content between thawed and fresh meat, but no significant differences was observed in protein content or hedonic scores
Downloads
References
Aini, N., & Pranoto, Y. (2021). Perspektif Global Ilmu dan Teknologi Pangan: Bab 3 Karakteristik Bahan Pangan. 1, 98–112. IPB Press.
Akhtar, S., Khan, M.I., & Faiz, F. (2013). Effect of thawing on frozen meat quality: A comprehensive review. Pakistan Journal of Food Sciences, 23(4), 198–211.
Ali, S., Rajput, N., Li, C., Zhang, W., & Zhou, G. (2016). Effect of freeze-thaw cycles on lipid oxidation and myowater in broiler chickens. Brazilian Journal of Poultry Science, 18(1), 35–40. https://doi.org/10.1590/1516-635x1801035-040
Arshad, M.W., Tariq, M.R., Ali, S.W., Basharat, Z., Umer, Z., Nayik, G.A., Ramniwas, S., Aloufi, A.S., Alharbi, S.A., Ansari, M.J., & Ercisli, S. (2023). Comparison of thawing treatments on quality, microbiota, and organoleptic characteristics of chicken meat fillets. ACS Omega, 8(29), 26548–26555. https://doi.org/10.1021/acsomega.3c03385
Augustyńska-Prejsnar, A., Hanus, P., Ormian, M., Kačániová, M., Sokołowicz, Z., & Topczewska, J. (2023). The effect of temperature and storage duration on the quality and attributes of the breast meat of hens after their laying periods. Foods, 12(23), 4340. https://doi.org/10.3390/foods12234340
Augustyńska-Prejsnar, A., Kačániová, M., Hanus, P., Sokołowicz, Z., & Słowiński, M. (2024). Microbial and sensory quality changes in broiler chicken breast meat during refrigerated storage. Foods, 13(24), 4063. https://doi.org/10.3390/foods13244063
Augustyńska-Prejsnar, A., Ormian, M., & Tobiasz-Salach, R. (2019). Quality of broiler chicken meat during frozen storage. Italian Journal of Food Science, 31(3).
Benli, H. (2016). Consumer attitudes toward storing and thawing chicken and effects of the common thawing practices on some quality characteristics of frozen chicken. Asian-Australasian Journal of Animal Sciences, 29(1), 100–108. https://doi.org/10.5713/ajas.15.0604
Badan Pengawas Obat dan Makanan (BPOM). (2021). Pedoman Cara Pengolahan dan Penanganan Pangan Olahan Beku yang Baik. Badan Pengawas Obat dan Makanan Republik Indonesia, Jakarta.
Bradford, M.M. (1976). A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1–2), 248–254. https://doi.org/10.1016/0003-2697(76)90527-3
Badan Standarisasi Nasional. (2002). SNI 01-3741-2022 tentang Minyak Goreng. BSN, Jakarta.
Badan Standarisasi Nasional. (2009). SNI 3924:2009 tentang Mutu Karkas dan Daging Ayam. BSN, Jakarta.
Chen, T.H., Zhu, Y.P., Han, M.Y., Wang, P., Wei, R., Xu, X.L., & Zhou, G.H. (2017). Classification of chicken muscle with different freeze–thaw cycles using impedance and physicochemical properties. Journal of Food Engineering, 196, 94–100. https://doi.org/10.1016/j.jfoodeng.2016.10.003
Deng, S., Liu, R., Li, C., Xu, X., & Zhou, G. (2022). Meat quality and flavor compounds of soft-boiled chickens: effect of Chinese yellow-feathered chicken breed and slaughter age. Poultry Science, 101(12), 102168. https://doi.org/10.1016/j.psj.2022.102168
Dewi, E.S., El Latifa, S., Fawwarahly, F., & Kautsar, R. (2016). Kualitas mikrobiologis daging unggas di RPA dan yang beredar di pasaran. Jurnal Ilmu Produksi dan Teknologi Hasil Peternakan, 4(3), 379-385. https://doi.org/10.29244/jipthp.4.3.379-385
Durack, E., Alonso-Gomez, M., & Wilkinson, M. (2012). The effect of thawing and storage temperature on the microbial quality of commercial frozen ready meals and experimental reduced salt frozen ready meals. Journal of Food Research, 1(2), 99–111. https://doi.org/10.5539/jfr.v1n2p99
Eastridge, J.S., & Bowker, B.C. (2011). Effect of rapid thawing on the meat quality attributes of USDA Select beef strip loin steaks. Journal of Food Science, 76(2), S156–S162. https://doi.org/10.1111/j.1750-3841.2010.02037.x
Edi, S., & Nur Rahmah, R.S. (2018). Pengaruh lama penyimpanan daging ayam pada suhu ruang dan refrigerator terhadap angka lempeng total bakteri dan adanya bakteri Salmonella sp. Jurnal Biosains, 4(1), 23.
Jemziya, F., & Rifath, A. (2022). Effect of different thawing techniques on certain quality parameters of different cut-up parts of broiler chicken. Egyptian Journal of Agricultural Research, 100(3), 330–335. https://doi.org/10.21608/ejar.2022.56344.1066
Kaewthong, P., Pomponio, L., Carrascal, J.R., Knøchel, S., Wattanachant, S., & Karlsson, A.H. (2019). Changes in the quality of chicken breast meat due to superchilling and temperature fluctuations during storage. Journal of Poultry Science, 56(4), 308–317. https://doi.org/10.2141/jpsa.0180106
Kassem, S., Yusuf, S., & Abd El-Malek, A.M. (2024). Effect of different thawing methods on sensory and physico-chemical properties of imported frozen beef. Assiut Veterinary Medical Journal, 70(180), 62–76. https://doi.org/10.21608/avmj.2023.227868.1177
Kim, G.-D., Jung, E.-Y., Lim, H.-J., Yang, H.-S., Joo, S.-T., & Jeong, J.-Y. (2013). Influence of meat exudates on the quality characteristics of fresh and freeze-thawed pork. Meat Science, 95(2), 323–329. https://doi.org/10.1016/j.meatsci.2013.05.007
Kominami, Y., Hayashi, T., Tokihiro, T., & Ushio, H. (2021). Peptidomic analysis characterising proteolysis in thaw-aging of beef short plate. Food Chemistry: Molecular Sciences, 3, 100051. https://doi.org/10.1016/j.fochms.2021.100051
Lee, S., Jo, K., Jeong, H.G., Choi, Y.-S., Kyoung, H., & Jung, S. (2022). Freezing-induced denaturation of myofibrillar proteins in frozen meat. Critical Reviews in Food Science and Nutrition, 64(5), 1385–1402. https://doi.org/10.1080/10408398.2022.2116557
Malak, N.M.L., & Soliman, N.S.M. (2021). The effect of time and temperature variations on the microbial load and deterioration criteria of leftover cheeseburger sandwiches. Advances in Animal and Veterinary Sciences, 9(11), 1925–1932. http://dx.doi.org/10.17582/journal.aavs/2021/9.11.1925.1932
Mardhika, H., Dwiloka, B., & Setiani, B.E. (2020). Pengaruh berbagai metode thawing daging ayam petelur afkir beku terhadap kadar protein, protein terlarut dan kadar lemak steak ayam. Jurnal Teknologi Pertanian, 4(1), 48-54.
Muttalib, S., Bintoro, N., Karyadi, J.N.W., & Saputro, A.D. (2025). Principal component analysis in the animal products precooling process using compressive type plate cooler. Jurnal Teknik Pertanian Lampung, 14(1), 182–193. https://doi.org/10.23960/jtep-l.v14i1.182-193
Oliveira, M.R., Gubert, G., Roman, S.S., Kempka, A.P., & Prestes, R.C. (2015). Meat quality of chicken breast subjected to different thawing methods. Revista Brasileira de Ciência Avícola, 17(2), 165–172. https://doi.org/10.1590/1516-635x1702165-172
Park, M.H., & Kim, M. (2024). Effects of thawing conditions on the physicochemical and microbiological quality of thawed beef. Preventive Nutrition and Food Science, 29(1), 80–86. https://doi.org/10.3746/pnf.2024.29.1.80
Pollack-Milgate, S., Saitia, S., & Tang, J.X. (2024). Rapid growth rate of Enterobacter sp. SM3 determined using several methods. BMC Microbiology, 24, 403. https://doi.org/10.1186/s12866-024-03547-3
Prehatini, D.A., Lestari, S.W., & Triasih, D. (2020). Pengaruh metode thawing terhadap kualitas fisik dan kimia daging sapi beku. Jurnal Ilmu dan Teknologi Peternakan Tropis, 7(1), 42-46. https://doi.org/10.33772/jitro.v7i1.8547
Putri, N., Lutpiatina, L., Nurlailah, N., & Insana, A. (2024). Differences in thawing methods in broiler chicken meat on total plate count (TPC) of bacteria. Tropical Health and Medical Research, 6(2), 21–28. https://doi.org/10.35916/thmr.v6i2.120
Qi, J., Li, C., Chen, Y., Gao, F., Xu, X., & Zhou, G. (2012). Changes in meat quality of ovine longissimus dorsi muscle in response to repeated freeze and thaw. Meat Science, 92(4), 619–626. https://doi.org/10.1016/j.meatsci.2012.06.009
Ragnarsson, S.Ö., & Viðarsson, J.R. (2017). Overview of available methods for thawing seafood. Matís report: Food Research, Innovation & Safety, 04-17, 1–38.
Rahman, M.H., Hossain, M.M., Rahman, S.M.E., Amin, M.R., & Oh, D.-H. (2015). Evaluation of physicochemical deterioration and lipid oxidation of beef muscle affected by freeze‑thaw cycles. Food Science of Animal Resources, 35(6), 772–782. https://doi.org/10.5851/kosfa.2015.35.6.772
Rakhmawati, A., Purwanto, Y.A., Widodo, S., & Astuti, D.A. (2023). Detection of formalin content in chicken meat using portable near infrared spectrometer. Jurnal Teknik Pertanian Lampung, 12(4), 831–839. https://doi.org/10.23960/jtep-l.v12i4.831-839
Ristanti, E.W., Kismiati, S., & Harjanti, D.W. (2017). Pengaruh lama pemaparan pada suhu ruang terhadap total bakteri, pH dan kandungan protein daging ayam di pasar tradisional Kabupaten Semarang. Jurnal Ilmu dan Teknologi Peternakan Tropis, 35(1).
Riyanti., & Dakhlan, A. (2022). Carcass percentage and organoleptic quality of Unila-1 superior chicken meat. AIP Conference Proceedings, 2563, 080002. https://doi.org/10.1063/5.0103502
Rosaini, H., Rasyid, R., & Hagramida, V. (2015). Penetapan kadar protein secara Kjeldahl beberapa makanan olahan kerang remis (Corbiculla moltkiana Prime.) dari Danau Singkarak. Jurnal Farmasi Higea, 7(2), 120–127.
Siswantoro, D., Busthomi, I., Suryadi, U., Prayitno, A.H., & Kusuma, S.B. (2023). Kualitas daging ayam broiler yang dijual di pasar tradisional Kabupaten Jember: Tingkat kontaminasi bakteri Escherichia coli, uji TPC, kadar air, dan nilai pH. Jurnal Triton, 14(2), 618-625. https://doi.org/10.47687/jt.v14i2.466
Stafford, C.D., Taylor, M.J., Spurling, R.A., Crump, Z.C., Alberto, A.F., Alruzzi, M.A., Ali, L.A., Okamoto, L.L., Bird, T.R., Page, C.M., Thornton, K.J., Dai, X., & Matarneh, S.K. (2024). The influence of different freezing and thawing conditions on the quality of beef rib primals. LWT, 209, 116771. https://doi.org/10.1016/j.lwt.2024.116771
Subakir M.F.N., Zaharudin N., Azman M.N.A., & Samat N. (2022). Thiobarbituric Reactive Substance (TBARS) and sensory evaluation of breast chicken meat from broiler fed with Kappaphycus alvarezii and Sargassum polycystum seaweeds formulated feed. Food Research, 6(2), 107-115. https://doi.org/10.26656/fr.2017.6(S2).022
Sujiwo, J., Jung, Y., Lee, S., Kim, D., Lee, H.-J., Oh, S., Kim, H.-J., Choo, H.-J., & Jang, A. (2025). The effect of different freezing and thawing methods on physicochemical, sensory, and flavor characteristics of korean native chicken breast. Food Science of Animal Resources, 45(2), 573–597. https://doi.org/10.5851/kosfa.2024.e110
Sun, Y., Luo, W., He, M., Zhao, Y., Sun, J., & Mao, X. (2024). Effects of different thawing methods on the physicochemical properties and myofibrillar protein characteristics of Litopenaeus vannamei. LWT, 192, 115668. https://doi.org/10.1016/j.lwt.2023.115668
Svendsen, E.S., Widell, K.N., Tveit, G.M., Nordtvedt, T.S., Uglem, S., Standal, I., & Greiff, K. (2022). Industrial methods of freezing, thawing and subsequent chilled storage of whitefish. Journal of Food Engineering, 315, 110803. https://doi.org/10.1016/j.jfoodeng.2021.110803
Wang, Y.-Y., Wang, H., Zhou, F., Wu, Y., Ma, H., Zhao, R., He, J., & Gu, Z. (2023). Effect of ultrasonic thawing temperature on the quality of quick-frozen small yellow croaker (Larimichthys polyactis) and its possible mechanisms. LWT, 179, 114620. https://doi.org/10.1016/j.lwt.2023.114620
Wu, X., Zhang, Z., He, Z., Wang, Z., Qin, F., Zeng, M., & Chen, J. (2021). Effect of freeze-thaw cycles on the oxidation of protein and fat and its relationship with the formation of heterocyclic aromatic amines and advanced glycation end products in raw meat. Molecules, 26(5), 1264. https://doi.org/10.3390/molecules26051264
Xia, X., Kong, B., Liu, J., Diao, X., & Liu, Q. (2012). Influence of different thawing methods on physicochemical changes and protein oxidation of porcine longissimus muscle. LWT - Food Science and Technology, 46(1), 280–286. https://doi.org/10.1016/j.lwt.2011.09.018
Yimenu, S.M., Koo, J., Kim, B.S., Kim, J.H., & Kim, J.Y. (2019). Freshness-based real-time shelf-life estimation of packaged chicken meat under dynamic storage conditions. Poultry Science, 98(12), 6921–6930. https://doi.org/10.3382/ps/pez461
Zhang, J., Bowker, B., Yang, Y., Pang, B., & Zhuang, H. (2020). Effects of deboning time and thawing method interaction on sensory descriptive profiles of cooked chicken breast and thigh meat. LWT, 120, 108939. https://doi.org/10.1016/j.lwt.2019.108939
Zhang, X., Gao, T., Song, L., Zhang, L., Jiang, Y., Li, J., Gao, F., & Zhou, G. (2017). Effects of different thawing methods on the quality of chicken breast. International Journal of Food Science and Technology, 52(9), 2097–2105. https://doi.org/10.1111/ijfs.13488
Zhang, Y., & Ertbjerg, P. (2018). Effects of frozen-then-chilled storage on proteolytic enzyme activity and water-holding capacity of pork loin. Meat Science, 145, 375–382. https://doi.org/10.1016/j.meatsci.2018.07.017
Zhou, P., & Xie, J. (2021). Effect of different thawing methods on the quality of mackerel (Pneumatophorus japonicus). Food Science and Biotechnology, 30, 1213–1223. https://doi.org/10.1007/s10068-021-00966-0

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


