Effect of Packaging on Protein Content, pH, and Sensory of Probiotic Tempeh Juice During Cold Storage
DOI:
https://doi.org/10.23960/jtepl.v14i6.2229-2238
Abstract View: 34
Keywords:
Cold storage, Packaging, Protein content, Shelf life, Tempeh juiceAbstract
Tempeh juice with probiotics is considered as functional probiotic foods with health benefits. This study aimed to evaluate the effect of packaging type (glass bottle and aluminum foil), and cold storage duration on chemical, microbial and sensory properties of probiotic tempeh juice. The research used a two-factorial Randomized Complete Block Design with three replications. ANOVA was used to analyze the data and it was followed by DMRT test at 5% significance level. The results showed that storage duration had no significant effect on viscosity and other chemical analysis, but was significant on sensory attributes of color, aroma, taste, and overall acceptability. Protein decreased on the 8th day probably due to protein hydrolysis by microbial activity, but increased on the 12th day of storage due to the accumulation of microorganism cell walls during storage. However, sensory changes included a paler color, a stronger fermentation aroma, an acidic alcoholic taste, and overall acceptance was acceptable. It was concluded that glass bottle packaging was more effective in maintaining the stability of protein content, pH, and sensory of probiotic tempeh juice beverage compared to the aluminum foil standing pouch with a spout, with the estimated shelf life was 8 days in cold storage.
Downloads
References
Abdullah, K., & Asriati, D.W. (2016). Karakteristik minuman sari tempe dengan penambahan rasa vanila. Journal of Agro-based Industry, 33(1), 1-8.
Aranda-González, I., Perera-Pacheco, M., Barbosa-Martín, E., & Betancur, D. (2016). Replacing sugar with S. rebaudiana extracts on the physicochemical and sensory properties of strawberry ice cream. Ciência Rural, 46(4), 604–609. https://doi.org/10.1590/0103-8478cr20150505
Archadiya, M., Radiati, L.E., & Sawitri, M.E. (2021). Pengaruh waktu penyimpanan seasoning whey kefir terhadap kualitas fisik, kimia dan mikrobiologis. Jurnal Agripet, 21(1), 35–39. https://doi.org/10.17969/agripet.v21i1.19406
Ayun, Q., Muthiáh, S. N., & Sukmalara, D. (2023). Potensi bakteri asam laktat (BAL) dari jus tempe sebagai kandidat probiotik. Jurnal Al-Azhar Indonesia, 8(2), 171–177. https://doi.org/10.36722/sst.v8i2.1673
BSN (Badan Standarisasi Nasional). (2006). SNI 01-2346-2006 – Petunjuk pengujian organoleptik dan atau sensori. Jakarta: Badan Standarisasi Nasional.
Bintari, S.H., Purnama, D.F.E., Saputro, D.D., Sunyoto, S., Dewi, P., & Mubarok, I. (2022). Microbiological and biochemical tests on tempe production using tempe mold innovation. Journal of Biology & Biology Education, 14(2), 245-253. https://doi.org/10.15294/biosaintifika.v14i2.37700
Budiningrum, E.W., Wahyudiyono, W., & Murdapa, P.A. (2022). Kemasan untuk meningkatkan kualitas dan penjualan produk. Hikmayo: Jurnal Pengabdian Masyarakat Amayo, 1(2), 58–67.
Darmawan, A., Suliasih, S., & Malianti, L. (2023). Pengaruh sari buah naga merah (Hylocereus polyrhizus L.) terhadap kandungan protein, karbohidrat, dan pH pada yoghurt drink dengan starter komersil. Jurnal Inspirasi Peternakan, 3(2), 64–72.
Devianti, V.A., & Arifiyana, D. (2022). Pengaruh bahan kemasan penyimpanan minuman infused water jeruk nipis terhadap kadar vitamin C. Journal Pharmasci (Journal of Pharmacy and Science), 7(2), 91–94. https://doi.org/10.53342/pharmasci.v7i2.293
Dhahana, K.A.P., Nocianitri, K.A., & Duniaji, A.S. (2021). Pengaruh lama fermentasi terhadap karakteristik soyghurt drink dengan penambahan Lactobacillus rhamnosus SKG 34. Jurnal Ilmu dan Teknologi Pangan (ITEPA), 10(4), 646-656. https://doi.org/10.24843/itepa.2021.v10.i04.p10
Fikriyah, H., Habibi, N., Ismanilda, I., Darningsih, S., Zulkifli, Z., Andrafikar, A., & Edmon, E. (2024). Pengaruh penambahan inulin terhadap mutu sensorik, kandungan serat, dan daya terima yoghurt. Jurnal Sehat Mandiri, 19(1), 369–379. https://doi.org/10.33761/jsm.v19i1.1389
Khazalina, T. (2020). Saccharomyces cerevisiae in making halal products based on conventional biotechnology and genetic engineering. Journal of Halal Product and Research (JHPR), 3(2), 88-94. https://doi.org/10.20473/jhpr.vol.3-issue.2.88-94
Kustyawati, M.E., Nurlita, M.E., Fadhallah, E.G., & Rizal, S. (2022). Prebiotic activity of Lactobacillus casei grown on medium containing hylocereus undatus extract and its use in the fermentation of goat's milk kefir. Biodiversity, 23(12), 6513-6519. https://doi.org/10.13057/biodiv/d231249
Isnaeni, Sugiyartono, Kusumawati, I., dan Rijal, M.A.S. (2016). Efek imunomodulator kombinasi susu probiotik dan ekstrak daun jambu biji. Jurnal Farmasi Indonesia, 8(1), 277–282.
Labiba, N.M., Marjan, A.Q., & Nasrullah, N. (2020). Pengembangan soyghurt (yoghurt susu kacang kedelai) sebagai minuman probiotik tinggi isoflavon. Amerta Nutrition, 4(3), 244–249. https://doi.org/10.20473/amnt.v4i3.2020.244-249
Lailia, R.P., Kentjonowaty, I., & Dinasari, I.R. (2023). Pengaruh lama simpan yoghurt susu kambing dengan penambahan sari jambu biji merah (Psidium guajava L.) terhadap total mikroba dan uji organoleptik. Dinamika Rekasatwa: Jurnal Ilmiah (e-Journal), 6(1), 177–184.
Lestari, Y., Amin, N., Ananda, D., & Rengganis, N.A. (2021). Identifikasi karakteristik kimiawi dan daya simpan kefir susu jagung (Zea mays L. Saccharata). Jurnal Gizi, 10(2), 20–32. https://doi.org/10.26714/jg.10.2.2021.20-32
Lozančić, M., Žunar, B., Hrestak, D., Lopandić, K., Teparić, R., & Mrša, V. (2021). Systematic comparison of cell wall-related proteins of different yeasts. Journal of Fungi, 7(2), 128. https://doi.org/10.3390/jof7020128
Mohammed, S.S. (2015) Effect of pH on the turbidity removal of wastewater. Open Access Library Journal, 2: e2283. http://dx.doi.org/10.4236/oalib.1102283
Muchtar, F., & Hastian, L.P. (2020). Pengaruh penyimpanan pada suhu rendah terhadap kandungan protein dan sifat organoleptik tahu. Jurnal Sains dan Teknologi Pangan, 5(5), 3256–3264.
Musoffin, A., Kentjonowaty, I., & Puspitarini, O.R. (2024). Pengaruh berbagai jenis gula terhadap nilai pH, sineresis dan kualitas organoleptik yoghurt. Jurnal Dinamika Rekasatwa, 7(1), 118–126.
Mustofa, Rohmaniyati, A., Gani, I.A., Sampurno, B., Ardi, B.N., Limbu, Y.S., Fatima, F., dan Hasanah, H. (2014). Pedoman Pemilihan Jenis Kemasan Pangan. Jakarta Pusat: Badan POM RI.
Nabilah, F.V., Listiyowati, S., dan Astuti, R.I. (2022). Diversitas pangan fermentasi berbasis susu di Indonesia dan kandungan gizinya. Jurnal Ilmu Pertanian Indonesia, 27(4), 552–561.
Nilakrisna, N., Patang, & Fadilah, R. (2024). Pengaruh jenis kemasan dan lama penyimpanan terhadap kualitas minuman pangan fungsional berbahan dasar jahe (Zingiber officinale). Jurnal Review Pendidikan dan Pengajaran, 7(3), 6607–6616.
Nirmalasari, R., Norrahmah, M., & Septina, N. (2023). Pengaruh suhu penyimpanan terhadap kualitas organoleptik minuman probiotik susu biji nangka (Artocarpus heterophyllus). Jurnal Ilmiah Biosaintropis (Bioscience-Tropic), 9(1), 70–80. https://doi.org/10.33474/e-jbst.v9i1.539
Nurhayati, N., Yuwanti, S., & Urbahillah, A. (2020). Karakteristik fisikokimia dan sensori kombucha cascara (kulit kopi ranum). Jurnal Teknologi dan Industri Pangan, 31(1), 38–49. https://doi.org/10.6066/jtip.2020.31.1.38
Nurman, M.N., Azhar, M., Amelia, F., & Oktavia, B. (2020). Pengaruh penambahan ekstrak inulin umbi dahlia terhadap karakteristik organoleptik sinbiotik set yoghurt. Periodic: Chemistry Journal Universitas Negeri Padang, 9(2), 55–59.
Pangestu, A.D., Kurniawan, dan Supriyadi. (2021). Pengaruh variasi suhu dan lama penyimpanan terhadap viabilitas bakteri asam laktat (BAL) dan nilai pH yoghurt. Borneo Journal of Medical Laboratory Technology (BJMLT), 3(2), 231–236.
Pradana, A.S., Srijuliani, E., & Risnantoko, W. (2018). Karakteristik kimia dan organoleptik yoghurt tempe dengan penambahan ekstrak buah bit (Beta vulgaris). Prosiding Seminar Nasional 5th FP "Pemanfaatan Sumber Daya Lokal Menuju Kemandirian Pangan Nasional", 1–8. Fakultas Pertanian, Universitas Veteran Bangun Nusantara.
Purry, A.P.K., & Rafiony, A. (2018). Pembuatan minuman sari tempe dengan ekstrak jeruk siam (Citrus nobilis) ditinjau dari mutu organoleptik kadar vitamin C dan kadar aktivitas antioksidan isoflavon. Prosiding Nasional Jurnal, 1(2), 60–65. https://doi.org/10.30602/pnj.v1i2.289
Rizal, S., & Kustyawati, M.E. (2019). Karakteristik organoleptik dan kandungan beta-glukan tempe kedelai dengan penambahan Saccharomyces cerevisiae. Jurnal Teknologi Pertanian, 20(2), 127–138.
Rizqiati, H., Susanti, S., Nurwantoro, Albaarri, A.N., & Slamet, Y.B. (2021). Pengaruh waktu fermentasi terhadap sifat fisiko kimia kefir whey dari susu kambing. Warta IHP, 38(1), 54–60.
Rosmawati, R., Syam, H., & Sukainah, A. (2021). Pengaruh jenis kemasan dan lama penyimpanan terhadap kualitas minuman khas Sinjai (Ires). Jurnal Pendidikan Teknologi Pertanian, 7(1), 79–92. https://doi.org/10.26858/jptp.v6i2.13107
Rossi, E., Hamzah, F., & Febriyani. (2016). Perbandingan susu kambing dan susu kedelai dalam pembuatan kefir. Jurnal Peternakan Indonesia, 18(1), 13–20. https://doi.org/10.25077/jpi.18.1.13-20.2016
Sabil, S., Amin, M., Maruddin, F., Husnaeni, H., & Taggo, S. (2023). Sifat fisiko-kimia dan fungsional susu kurma dengan penambahan bakteri asam laktat pada level berbeda. Jurnal Ilmu dan Industri Peternakan, 9(2), 203–213. https://doi.org/10.24252/jiip.v9i2.41824
Santoso, T.L.A., Wicaksono, D.A., & Gunawan, P.N. (2022). Effects of carbonated soft drink on saliva pH in the occurrence of dental caries. E-GiGi, 10(1), 66–74. https://doi.org/10.35790/eg.v10i1.37606
Sudarmadji, S., Haryono, B., Suhardi. (1997). Prosedur Analisa Untuk Bahan Makanan dan Pertanian. Yogyakarta : Liberty.
Sukmaningrum, H., Darmayanti, T., & Puspawati, G.A.K.D. (2021). The changes of characteristic fermented milk during room temperature storage. Jurnal Ilmu dan Teknologi Pangan (ITEPA), 10(1), 119–130. https://doi.org/10.24843/itepa.2021.v10.i01.p11
Teame, T., Wang, A., Xie, M., Zhang, Z., Yang, Y., Ding, Q., Gao, C., Olsen, R.E., Ran, C., & Zhou, Z. (2020). Paraprobiotics and postbiotics of probiotic Lactobacilli, their positive effects on the host and action mechanisms: A review. Frontiers in Nutrition, 7, 570344. https://doi.org/10.3389/fnut.2020.570344
Tefa, P., Ledo, M.E.S., & Nitsae, M. (2023). Variasi konsentrasi Saccharomyces cerevisiae dalam pembuatan wine buah dilak (Limonia acidissima). SCISCITATIO, 4(1), 32–38. https://doi.org/10.21460/sciscitatio.2023.41.112
Tritisari, A., Lianto, A., & Suhana, S. (2024). Pengaruh variasi konsentrasi natrium benzoat terhadap kualitas dan daya simpan sirop nanas. Jurnal Teknologi Pangan dan Industri Perkebunan (LIPIDA), 4(2), 41–50. https://doi.org/10.58466/lipida.v4i2.1678
Tursina, T., Irfan, I., & Anwar, S.H. (2019). Tingkat penerimaan panelis terhadap yoghurt dengan perlakuan lama fermentasi, jenis susu, dan lama penyimpanan yang berbeda. Jurnal Ilmiah Mahasiswa Pertanian, 4(3), 65–74. https://doi.org/10.17969/jimfp.v4i3.11637
Udin, J., Nurlaelah, I., & Priyanto, A. (2020). Pengaruh kadar konsentrasi Saccharomyces cerevisiae terhadap sifat organoleptik dan sifat kimia (alkohol dan gula) pada brem cair Ipomea batatas L. EDUBIOLOGICA: Jurnal Penelitian Ilmu dan Pendidikan Biologi, 8(1), 25–34.
Usman, M., Tarigan, B.Y., Aprilia, M., Zalvi, A.P., Sari, F.I., Romauli, N.D.M., & Sinaga, H. (2023). Pengujian daya terima (uji hedonik) pada empat merek produk yoghurt yang dijual pada pasar modern (supermarket) di Kecamatan Medan Kota. Jurnal Agroindustri Pangan, 2(2), 1–16. https://doi.org/10.47767/agroindustri.v2i2.523
Walker, G.M., & Stewart, G.G. (2016). Saccharomyces cerevisiae in the production of fermented beverages. Beverages, 2(4), 30. https://doi.org/10.3390/beverages2040030
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Nur Aini Sahara, Nur Aini Sahara, Attika Nur Aisah, Maria Erna Kustyawati, Sussi Astuti, Wisnu Satyajaya

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Authors who publish with this journal agree to the following terms:
Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution-ShareAlike 4.0 International Lice that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (See The Effect of Open Access).
Jurnal Teknik Pertanian Lampung

JTEPL is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.


