Optimization of Bioencapsulation of Bacillus sp. to Increase Resistance to Bacterial Wilt and Growth of Cayenne Pepper

Authors

  • Ajeng Distya Anjani Universitas Pembangunan Nasional "Veteran" Jawa Timur
  • Yenny Wuryandari Universitas Pembangunan Nasional "Veteran" Jawa Timur
  • Safira Rizka Lestari Universitas Pembangunan Nasional "Veteran" Jawa Timur

DOI:

https://doi.org/10.23960/jtepl.v14i4.1325-1334
Abstract View: 101

Keywords:

Bacillus sp., Bacterial wilt, Cayenne pepper, Gelatine, Sodium alginate

Abstract

Cayenne pepper has a high production demand. The high demand does not match the amount of production produced. One of the factors for decreased production is the presence of bacterial wilt disease caused by Ralstonia solanacearum. Bacterial wilt control can be done with the application of antagonistic bacteria, namely Bacillus sp. Bacillus sp. application requires an optimal formulation to maintain bacterial activity in the soil, one of which is Bacillus sp. encapsulation. This study aims to determine the effectiveness of Bacillus sp. encapsulation as a formulation of biological agent bacteria for the control of bacterial wilt disease and cayenne pepper plant growth. This study used the encapsulating material concentration and dosage factors for application to plants. Encapsulation was made using the extrusion method. The results showed that the concentration of 2% sodium alginate and 1.5% gelatin gave a significant difference in disease intensity of 18.89% and in the growth parameters of cayenne pepper plants were able to reach a height of 30.58 cm and a root length of 17.18 cm. The right concentration and high dose provide the ability to suppress intensity and encourage maximum growth

Downloads

Download data is not yet available.

References

Arif, A. (2015). Pengaruh bahan kimia terhadap penggunaan pestisida lingkungan. Jurnal Farmasi UIN Alauddin Makassar, 3(4), 134-143.

Arwiyanto, T., & Hartana, I. (1999). Pengendalian hayati penyakit layu bakteri tembakau : 2. Percobaan di rumah kaca. Jurnal Perlindungan Tanaman Indonesia, 5(1), 50-59.

BPS (Badan Pusat Statistik). (2023). Statistik Indonesia 2022. Badan Pusat Statistik, Jakarta.

Bashan, Y., de-Bashan, L.E., Prabhu, S.R., & Hernandez, J.P. (2014). Advances in plant growth-promoting bacterial inoculant technology: Formulations and practical perspectives (1998–2013). Plant and Soil, 378, 1–33. https://doi.org/10.1007/s11104-013-1956-x

Choliq, F.A., Martosudiro, M., Istiqomah, I., & Nijami, M.F. (2020). Isolasi dan uji kemampuan bakteriofag sebagai agens pengendali penyakit layu bakteri (Ralstonia solanacearum) pada tanaman tomat. VIABEL: Jurnal Ilmiah Ilmu-Ilmu Pertanian, 14(1), 8-20.

Dalsing, B.L., & Allen, C. (2014). Nitrate assimilation contributes to Ralstonia solanacearum root attachment, stem colonization, and virulence. Journal of Bacteriology, 196(5), 949–960. https://doi.org/10.1128/JB.01378-13

Djereng, D.K., Kawuri, R., & Ramona, Y. (2017). Potensi Bacillus sp. B3 sebagai agen biokontrol penyakit layu bakteri yang disebabkan oleh Ralstonia sp. pada tanaman cabai (Capsicum annuum L.). Metamorfosa: Journal of Biological Sciences, 4(2), 237–237. https://doi.org/10.24843/metamorfosa.2017.v04.i02.p16

Diyasti, F., & Lizarmi, E. (2021). Kajian Penggunaan Antibiotik pada Komoditas Perkebunan. AGROSCRIPT: Journal of Applied Agricultural Sciences, 3(2), 99-112.

Ezhilarasi, P.N., Karthik, P., Chhanwal, N., & Anandharamakrishnan, C. (2013). Nanoencapsulation techniques for food bioactive components: A review. Food and Bioprocess Technology, 6, 628–647. https://doi.org/10.1007/s11947-012-0944-0

Haryani, T.S., & Tombe, O.M. (2017). Pemanfaatan bakteri antagonis terhadap pengendalian jamur patogen Fusarium oxysporum dan Phytopthora capsici secara in vitro. Ekologia: Jurnal Ilmiah Ilmu Dasar dan Lingkungan Hidup, 11(2), 11-21.

Hasyim, A., Setiawati, W., & Lukman, L. (2015). Inovasi teknologi pengendalian OPT ramah lingkungan pada cabai: Upaya alternatif menuju ekosistem harmonis. Pengembangan Inovasi Pertanian, 8(1), 1–10.

Husna, M., Sugiyanta, S., & Pratiwi, E. (2019). Kemampuan konsorsium Bacillus pada pupuk hayati dalam memfiksasi N2, melarutkan fosfat, dan mensintesis fitohormon Indole 3-Acetic Acid. Jurnal Tanah dan Iklim, 43(2), 117–125.

Istiqomah, I., Aini, L.Q., & Abadi, A.L. (2017). Kemampuan Bacillus subtilis dan Pseudomonas fluorescens dalam melarutkan fosfat dan memproduksi hormon IAA (Indole Acetic Acid) untuk meningkatkan pertumbuhan tanaman tomat. Buana Sains, 17(1), 75. https://doi.org/10.33366/bs.v17i1.580

Khimmakthong, U., Khumpouk, P., Saichanaphan, N., Intarasin, Y., & Tirawanichakul, K. (2020). The efficiency of microencapsulation with alginat, gelatin, and chitosan on the survival of Bacillus subtilis. Chiang Mai University Journal of Natural Sciences, 19(4), 684-701.

Kurabachew, H., & Ayana, G. (2017). Bacterial wilt caused by Ralstonia solanacearum in Ethiopia: Status and management approaches: A review. International Journal of Phytopathology, 5(3), 107–119. http://dx.doi.org/10.33687/phytopath.005.03.1829

Lede, N., Muchtar, R., & Sholihah, S.M. (2018). Respon pertumbuhan dan hasil tanaman cabai rawit (Capsicum frutescens L.) terhadap penggunaan trichokompos pada pemupukan berimbang. Jurnal Ilmiah Respati, 9(2).

McGarvey, J.A., Denny, T.P., & Schell, M.A. (1999). Spatial-temporal and quantitative analysis of growth and EPS I production by Ralstonia solanacearum in resistant and susceptible tomato cultivars. Phytopathology, 89, 1233–1239. http://dx.doi.org/10.1094/PHYTO.1999.89.12.1233

Pour, M.M., Saberi-Riseh, R., Mohammadinejad, R., & Hosseini, A. (2019). Investigating the formulation of alginate-gelatin encapsulated Pseudomonas fluorescens (VUPF5 and T17-4 strains) for controlling Fusarium solani on potato. International Journal of Biological Macromolecules, 133, 603–611. https://doi.org/10.1016/j.ijbiomac.2019.04.071

Pour, M.M., Saberi-Riseh, R., Esmaeilzadeh-Salestani, K., Mohammadinejad, R., & Loit, E. (2021). Evaluation of Bacillus velezensis for biological control of Rhizoctonia solani in bean by alginate/gelatin encapsulation supplemented with nanoparticles. Journal of Microbiology and Biotechnology, 31(10), 1373. https://doi.org/10.4014/jmb.2105.05001

Raihanah, R., Fitriyanti, D., & Liestiany, E. (2023). Pengujian beberapa varietas cabai besar (Capsicum annuum L.) terhadap lama periode inkubasi dan tingkat ketahanannya terhadap layu bakteri Ralstonia solanacearum. Jurnal Proteksi Tanaman Tropika, 6(3), 747-755.

Saputra, M.M., Wuryandari, Y., & Rahmadhini, N. (2024). Pengujian biologis formulasi bioenkapsulasi Bacillus sp. untuk menghambat penyakit layu bakteri pada tanaman cabai. Jurnal Agroekoteknologi, 16(1), 1–12.

Setiaji, A., Annisa, R.R.R., & Rahmandhias, D.T. (2023). Bakteri Bacillus sebagai agen kontrol hayati dan biostimulan tanaman. Rekayasa, 16(1), 96–106. https://doi.org/10.21107/rekayasa.v16i1.17207

Skopinska-Wisniewska, J., Tuszynska, M., Kaźmierski, Ł., Bartniak, M., & Bajek, A. (2024). Gelatin–sodium alginate hydrogels cross-linked by squaric acid and dialdehyde starch as a potential bio-ink. Polymers, 16(18), 2560. https://doi.org/10.3390/polym16182560

Tomić, S.L., Babić Radić, M.M., Vuković, J.S., Filipović, V.V., Nikodinovic-Runic, J., & Vukomanović, M. (2023). Alginate-based hydrogels and scaffolds for biomedical applications. Marine Drugs, 21(3), 177. https://doi.org/10.3390/md21030177

Wulandasari, D., Al-Awwaly, K.U., & Manab, A. (2022). Microencapsulated Lactobacillus acidophilus FNCC 0051 and Streptococcus thermophilus FNCC 0040 technique emulsion using gelatin and sodium alginate. Asian Food Science Journal, 21(4), 1–9. https://doi.org/10.9734/afsj/2022/v21i430420

Wartono, G., & Mutaqin, K.H. (2014). Efektivitas formulasi spora Bacillus subtilis B12 sebagai agen pengendali hayati penyakit hawar daun bakteri pada tanaman padi. Jurnal Penelitian Pertanian Tanaman Pangan, 34(1), 21–28.

Zinidin, M. (2022). Eksplorasi Bacillus spp. Pada Rhizosfer Cabai Merah (Capsicum annuum L.) Dataran Tinggi Dan Potensinya Sebagai Agensia Pengendali Hayati Patogen Ralstonia solanacearum Secara In Vitro. [Undergraduate Thesis]. Universitas Pembangunan Nasional “Veteran” Jawa Timur.

Downloads

Published

2025-07-25

How to Cite

Anjani, A. D., Wuryandari, Y., & Lestari, S. R. (2025). Optimization of Bioencapsulation of Bacillus sp. to Increase Resistance to Bacterial Wilt and Growth of Cayenne Pepper. Jurnal Teknik Pertanian Lampung (Journal of Agricultural Engineering), 14(4), 1325–1334. https://doi.org/10.23960/jtepl.v14i4.1325-1334