Effect of Aeration Rate on the Quality of Bio-Urine Produced from Cattle Urine Fermentation in an Aerobic Bioreactor
Abstract
The utilization of cattle urine as a raw material for liquid organic fertilizer has strong potential to support sustainable agriculture through the optimization of aerobic fermentation using a bioreactor system. This study aimed to analyze the effect of aeration rate on the quality of bio urine produced. The fermentation process was conducted for 15 days in a 1100 L aerobic bioreactor with three aeration rate treatments of 48, 60, and 90 L/h. The main parameters evaluated included pH, biological oxygen demand (BOD), C/N ratio, microbial population, and macro-nutrient contents (N, P, and K). The results showed that increasing the aeration rate accelerated the reduction of BOD and C/N ratio while maintaining pH stability throughout the fermentation process. The aeration rate of 90 L/h produced bio urine with a C/N ratio below 15, the lowest BOD value, and stable pH, indicating the highest level of fermentation maturity and chemical stability. Under this condition, macro-nutrient contents reached 0.25% N, 0.10% P, and 0.42% K, accompanied by a stable microbial population during the final fermentation phase. Therefore, an aeration rate of 90 L/h was determined as the optimal condition based on fermentation maturity indicators (C/N ratio, BOD, and pH) and improved efficiency of organic matter mineralization.
Downloads
References
Ahmadi, M., Doroodmand, M. M., Bidhendi, G. N., Torabian, A., & Mehrdadi, N. (2022). Efficient wastewater treatment via aeration through a novel nanobubble system in sequence batch reactors. Frontiers in Energy Research, 10, 884353. https://doi.org/10.3389/fenrg.2022.884353
Ali, N., Wang, S., Zhao, J., Dong, Z., Li, J., Nazar, M., & Shao, T. (2020). Microbial diversity and fermentation profile of red clover silage inoculated with reconstituted indigenous and exogenous epiphytic microbiota. Bioresource Technology, 314, 123606. https://doi.org/10.1016/j.biortech.2020.123606
Ambigalakshmi, N., Raghavi, M.P., Yogha Sri, S., & Indianraj, N. (2023). Cow urine: A potential benefits and uses in agriculture. International Journal of Advanced Research in Science, Communication and Technology, 3(2). https://doi.org/10.48175/IJARSCT-8377
Asadu, C.O., Ike, I.S., Onu, C.E., Egbuna, S.O., Onoh, M.O., Mbah, G.O., & Eze, C.N. (2020). Investigation of the influence of biofertilizer synthesized using microbial inoculums on the growth performance of two agricultural crops. Biotechnology Reports, 27, e00493. https://doi.org/10.1016/j.btre.2020.e00493
Chidi, B.S., Bauer, F.F., & Rossouw, D. (2018). Organic acid metabolism and the impact of fermentation practices on wine acidity: A review. South African Journal of Enology and Viticulture, 39(2), 315–329. https://doi.org/10.21548/39-2-3172
Duan, H., Ji, M., Xie, Y., Shi, J., Liu, L., Zhang, B., & Sun, J. (2021). Exploring the microbial dynamics of organic matter degradation and humification during co-composting of cow manure and bedding material waste. Sustainability, 13(23), 13035. https://doi.org/10.3390/su132313035
Fu, C., Ma, W., Qiang, B., Jin, X., Zhang, Y., & Wang, M. (2023). Effect of chemical fertilizer with compound microbial fertilizer on soil physical properties and soybean yield. Agronomy, 13(10), 2488. https://doi.org/10.3390/agronomy13102488
Gao, D., Bai, E., Wasner, D., & Hagedorn, F. (2024). Global prediction of soil microbial growth rates and carbon use efficiency based on the metabolic theory of ecology. Soil Biology and Biochemistry, 190, 109315. https://doi.org/10.1016/j.soilbio.2024.109315
Ge, M., Zhou, H., Shen, Y., Meng, H., Li, R., Zhou, J., Cheng, H., Zhang, X., Ding, J., Wang, J., & Wang, J. (2020). Effect of aeration rates on enzymatic activity and bacterial community succession during cattle manure composting. Bioresource Technology, 304, 122928. https://doi.org/10.1016/j.biortech.2020.122928
Jiang, Z., Lu, Y., Xu, J., Li, M., Shan, G., & Li, Q. (2019). Exploring the characteristics of dissolved organic matter and succession of bacterial community during composting. Bioresource Technology, 292, 121942. https://doi.org/10.1016/j.biortech.2019.121942
Kadam, R., Jo, S., Lee, J., Khanthong, K., Jang, H., & Park, J. (2024). A review on the anaerobic co-digestion of livestock manures in the context of sustainable waste management. Energies, 17(3), 546. https://doi.org/10.3390/en17030546
Kurniawan, L., Maryudi, M., & Astuti, E. (2024). Utilization of tofu liquid waste as liquid organic fertilizer using the fermentation method with effective activator microorganisms 4 (EM-4): A review. Equilibrium, 8(1). https://doi.org/10.20961/equilibrium.v8i1.84056
Kurzemann, F.R., Plieger, U., Probst, M., Spiegel, H., Sandén, T., Ros, M., & Insam, H. (2020). Long-term fertilization affects soil microbiota, improves yield and benefits soil. Agronomy, 10(11), 1664. https://doi.org/10.3390/agronomy10111664
Li, L., Xu, X., Wang, W., Lau, R., & Wang, C.-H. (2022). Hydrodynamics and mass transfer of concentric-tube internal loop airlift reactors: A review. Bioresource Technology, 359, 127451. https://doi.org/10.1016/j.biortech.2022.127451
Liu, S., Zhao, J., Feng, W.-L., Zhang, Z.-J., Gu, Y.-F., & Wang, Y.-P. (2024). Microbial community succession of cow manure and tobacco straw composting. Frontiers in Microbiomes, 3, 1301156. https://doi.org/10.3389/frmbi.2024.1301156
López-Rubio, J.F., Cebrián-Tarancón, C., Alonso, G.L., Salinas, M.R., & Sánchez-Gómez, R. (2025). Preparation and characterization of liquid fertilizers produced by anaerobic fermentation. Agriculture, 15(11), 1225. https://doi.org/10.3390/agriculture15111225
Mal, S., & Chattopadhyay, G.N. (2024). Influence of aeration through turning on microbial activity and decomposition rate in vermicomposting. Asian Journal of Microbiology and Biotechnology, 9(2), 108–114. https://doi.org/10.56557/ajmab/2024/v9i28904
Mudhita, I.K., Saprudin, Mulyadi, D., & Nurlatipah, S. (2023). The effect of giving liquid organic fertilizer made from cow urine enriched by agricultural symbiotic microbes on the production of Taiwan grass (Pennisetum purpureum cv. Mott). Borneo Journal of Animal Science, 5(2). https://doi.org/10.32585/bjas.v5i2.4652
Nuraini, Y., & Asgianingrum, R.E. (2017). Peningkatan kualitas biourin sapi dengan penambahan pupuk hayati dan molase serta pengaruhnya terhadap pertumbuhan dan produktivitas pakchoy. Jurnal Hortikultura Indonesia, 8(3), 183–191. https://doi.org/10.29244/jhi.8.3.183-191
Oji, A., & Godgift, D.R. (2019). Effect of biofertilizer produced from cow dung and conventional fertilizer on plant growth. International Journal of Advanced Engineering Research and Science, 6(11), 535–540. https://doi.org/10.22161/ijaers.611.80
Oliveira da Silva, D.C., Nascimento, E.N., Oliveira da Silva, A., Uchôa, S.C.P., & Barreto, G.F. (2023). Effect of biofertilization with cattle urine on the chemical properties of an Oxisol from the Amazon savanna. Pesquisa Agropecuária Tropical, 53. https://doi.org/10.1590/1983-40632023v5375568
Ortiz-Marin, A.D., Roé-Sosa, A., Solis-Marcial, O.J., Chavarría, J., Mendivil-Garcia, K., & Amabilis-Sosa, L.E. (2025). Nutrient-efficient recovery process from real livestock wastewater using UV/H₂O₂ and UV/PMS: Kinetic study and statistical optimization. Journal of Water Process Engineering, 74, 107811. https://doi.org/10.1016/j.jwpe.2025.107811
Perwez, M., & Al Asheh, S. (2025). Valorization of agro-industrial waste through solid-state fermentation: Mini review. Biotechnology Reports, 45, e00873. https://doi.org/10.1016/j.btre.2024.e00873
Pradhan, S.S., Verma, S., Kumari, S., & Singh, Y. (2018). Bio-efficacy of cow urine on crop production: A review. International Journal of Chemical Studies, 6(3), 298–301.
Pramudya, Y., Ibat, M.I., & Ingesti, P.S.V.R. (2024). The effect of liquid organic fertilizer from cow urine with monosodium glutamate dosage on the growth of Cening and Bululawang sugarcane bud sets. Open Soil Science and Environment, 2(2), 71–79.
Puspitaloka, H., Mimoto, H., Tran, Q.N.M., Koyama, M., & Nakasaki, K. (2022). Effect of aeration methods on the organic matter degradation, microbial community and their catabolic function during composting. Waste and Biomass Valorization, 13, 1195–1205. https://doi.org/10.1007/s12649-021-01560-5
Putra, G.J.K., Setiyo, Y., & Sucipta, I.N. (2022). Pengaruh penambahan bakteri nitrifikasi pada fermentasi urin sapi terhadap kualitas pupuk organik cair. Jurnal BETA (Biosistem dan Teknik Pertanian), 10(1). https://doi.org/10.24843/JBETA.2022.v10.i01.p02
Ribera-Guardia, A., & Pijuan, M. (2017). Distinctive NO and N₂O emission patterns in ammonia oxidizing bacteria: Effect of ammonia oxidation rate, DO and pH. Chemical Engineering Journal, 321, 358–365. https://doi.org/10.1016/j.cej.2017.03.122
Ruiz, E.D., Arora, R., dos Santos, J.C., da Silva, S.S., & Chandel, A.K. (2025). Cow urine is a potent organic nitrogen source for microbial production of ethanol and β-carotene: an experimental proof. Catal, 1(4). https://doi.org/10.1007/s44422-025-00002-w
Setiyo, Y., Harsojuwono, B.A., Gunam, I.B.W., Gunadnya, I.B.P., Aviantara, I.G.N.A., & Triani, I.G.A.L. (2023). Performance of bubble column bioreactor to produce liquid organic fertilizer (LOF) from cow urine and slurry organic waste. Jurnal Teknologi, 85(4). https://doi.org/10.11113/jurnalteknologi.v85.18539
Situmeang, D.J., Girsang, R., & Sulastri, T. (2019). The influence of cow urine fertilizer, leaf bokashi, and AB mix for the growth of water spinach plant (Ipomoea reptans var. poir) with the DFT (deep flow technique) hydroponic system at Adventist University of Indonesia. International Scholars Conference, 7(1), 1879-7894. https://doi.org/10.35974/isc.v7i1.1981
Sun, H., Cui, X., Li, R., Guo, J., & Dong, R. (2021). Ensiling process for efficient biogas production from lignocellulosic substrates: Methods, mechanisms, and measures. Bioresource Technology, 341, 125928. https://doi.org/10.1016/j.biortech. 2021.125928
Sun, L., Guan, W., Tai, X., Qi, W., Zhang, Y., Ma, Y., Sun, X., Lu, Y., & Lin, D. (2025). Research progress on microbial nitrogen conservation technology and mechanism of microorganisms in aerobic composting. Microbial Ecology, 88(19). https://doi.org/10.1007/s00248-025-02513-4
Tabbassum, R., Naveed, M., Mehboob, I., Babar, M.H., Holatko, J., Akhtar, N., Rafique, M., Kucerik, J., Brtnicky, M., Kintl, A., Vyhnanek, T., & Mustafa, A. (2022). Comparative response of fermented and non-fermented animal manure combined with split dose of phosphate fertilizer enhances agronomic performance and wheat productivity through enhanced P use efficiency. Agronomy, 12(10), 2335. https://doi.org/10.3390/agronomy12102335
Tang, J., Zhang, S., Zheng, G., Han, Z., Wang, D., & Lin, H. (2024). Role of bioavailability in compost maturity during aerobic composting of chicken manure. Sustainability, 16(24), 11122. https://doi.org/10.3390/su162411122
Urra, J., Alkorta, I., Mijangos, I., & Garbisu, C. (2020). Commercial and farm fermented liquid organic amendments to improve soil quality and lettuce yield. Journal of Environmental Management, 264, 110422. https://doi.org/10.1016/j.jenvman.2020.110422
Wang, Y., Tang, Y., Li, M., & Yuan, Z. (2021). Aeration rate improves the compost quality of food waste and promotes the decomposition of toxic materials in leachate by changing the bacterial community. Bioresource Technology, 340, 125716. https://doi.org/10.1016/j.biortech.2021.125716
Wang, Y., Xu, P., Wang, Y., Su, J., Xu, Z., Jiang, Z., Wei, Y., Hang, S., Ding, X., Zhang, H., Zhang, L., Liu, Y., & Li, J. (2024). Effects of aeration modes and rates on nitrogen conversion and bacterial community in composting of dehydrated sludge and corn straw. Frontiers in Microbiology, 15, 1372568. https://doi.org/10.3389/fmicb.2024.1372568
Xing, Y., Xie, Y., & Wang, X. (2025). Enhancing soil health through balanced fertilization: a pathway to sustainable agriculture and food security. Frontiers in Microbiology, 16, 1536524. https://doi.org/10.3389/fmicb.2025.1536524
Xiong, Z.Q., Wang, G.X., Huo, Z.C., Yan, L., Gao, Y.M., Wang, Y.J., Gu, J.D., & Wang, W.D. (2017). Effect of aeration rates on the composting processes and nitrogen loss during composting. Applied Environmental Biotechnology, 2(2), 20–27. https://doi.org/10.26789/AEB.2017.01.003
Yang, M., Guo, Y., Yang, F., Wang, J., Gao, Y., Wang, M., Liang, X., & He, S. (2024). Dynamic changes in and correlations between microbial communities and physicochemical properties during the composting of cattle manure with Penicillium oxalicum. BMC Microbiology, 24, 301. https://doi.org/10.1186/s12866-024-03449-4
Yunus, M.U., Silas, K., Yaumi, A.L., & Kwaji, B.H. (2022). A review of biofertilizer production: Bioreactor, feedstocks and kinetics. International Journal of Recent Engineering Science, 9(1), 39–50. https://doi.org/10.14445/23497157/IJRES-V9I1P106
Zhao, L., Huang, Y., Ran, X., Xu, Y., Chen, Y., Wu, C., & Tang, J. (2025). Nitrogen transformation mechanisms and compost quality assessment in sustainable mesophilic aerobic composting of agricultural waste. Sustainability, 17(2), 575. https://doi.org/10.3390/su17020575
Zhen, L.M., Ho, Y.C., & Tai, Y.L. (2025). Comprehensive analysis of aerobic and anaerobic biological treatments in industrial wastewater: Mechanisms, advantages, and critical limitations. International Journal of Biomass & Renewables, 14(1), 31–48. https://doi.org/10.61762/ijbrvol14iss1art004
Zhou, Y., Shi, S., Zhou, J., He, L., He, X., Lu, Y., He, Q., & Zhou, J. (2022). Composition characterization and transformation mechanism of dissolved organic matters in a full-scale membrane bioreactor treating co-digestion wastewater of food waste and sewage sludge. Sustainability, 14(11), 6556. https://doi.org/10.3390/su14116556

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.



ARTICLE TEMPLATE