Enhancing Growth and Yield of Garuda Clone Cassava through Mukibat Grafting and Microbial-Based Fertilization
Abstract
Cassava (Manihot esculenta Crantz) is a strategic commodity in Indonesia, but the productivity (20–30 t/ha) remains far below the biological potential (>60 t/ha). Mukibat grafting, which combines rubber cassava (Manihot glaziovii) as scion onto cultivated cassava rootstock, represents an effective approach to enhance productivity. This study aimed to evaluate the effects of planting materials (Mukibat grafting vs. conventional cutting) and fertilizer types (inorganic NPK, goat manure, and microbial-based fertilizer/MBF) on the growth and yield of Garuda clone cassava. The experiment was conducted using a Strip Plot Design with a 2×3 factorial treatment structure and three replications. Results showed that grafting success rate was 100% across all treatments. Mukibat grafting significantly outperformed conventional cuttings, increasing tuber yield by 98.14% (61.70 kg vs. 31.14 kg per 3 plants) and total starch production by 102.77% (50.49 vs. 24.90 t/ha). Starch content did not differ significantly between treatments. MBF produced the highest tuber yield (52.40 kg per 3 plants) and total starch production (42.95 t/ha), representing a 31.07% and 28.81% increase over inorganic fertilizer (39.98 kg per 3 plants), respectively. These findings demonstrate that integrating Mukibat grafting with microbial-based fertilization represents an effective strategy for sustainable intensification of cassava production in Indonesia.
Downloads
References
Aasfar, A., Bargaz, A., Yaakoubi, K., Hilali, A., Bennis, I., Zeroual, Y., & Kadmiri, I.M. (2021). Nitrogen fixing Azotobacter species as potential soil biological enhancers for crop nutrition and yield stability. Frontiers in Microbiology, 12, 628379. https://doi.org/10.3389/fmicb.2021.628379
Bangthong, P., Vuttipongchaikij, S., Kongsil, P., Ceballos, H., & Kittipadakul, P. (2022). Evaluation of Manihot glaziovii scion-cassava understock grafting for cassava growth and root yield during rainy and dry seasons. Journal of Crop Improvement, 36(2), 193–206. https://doi.org/10.1080/15427528.2021.1931609
Bhatt, M.K., Labanya, R., & Joshi, H.C. (2019). Influence of long-term chemical fertilizers and organic manures on soil fertility: A review. Universal Journal of Agricultural Research, 7(5), 177–188. https://doi.org/10.13189/ujar.2019.070502
BPS (Badan Pusat Statistik). (2024). Statistik Pertanian 2024 (Agricultural Statistics 2024). Pusat Data dan Sistem Informasi Pertanian, Kementerian Pertanian Republik Indonesia, Jakarta. ISBN: 978-602-6726-68-8.
Cai, J., Zhang, J., Ding, Y., Yu, S., Lin, H., Yuan, Z., Li, K., Ou, W., & Chen, S. (2021). Different fertilizers applied alter fungal community structure in rhizospheric soil of cassava (Manihot esculenta Crantz) and increase crop yield. Frontiers in Microbiology, 12, 663781. https://doi.org/10.3389/fmicb.2021.663781
Chiewchankaset, P., Thaiprasit, J., Kalapanulak, S., Wojciechowski, T., Boonjing, P., & Saithong, T. (2022). Effective metabolic carbon utilization and shoot-to-root partitioning modulate distinctive yield in high yielding cassava variety. Frontiers in Plant Science, 13, 832304. https://doi.org/10.3389/fpls.2022.832304
Cock, J.H., Franklin, D., Sandoval, G., & Juri, P. (1979). The ideal cassava plant for maximum yield. Crop Science, 19(2), 271–279. https://doi.org/10.2135/cropsci1979.0011183X001900020025x
Dalorima, T., Sakimin, S.Z., & Shah, R.M. (2021). Utilization of organic fertilisers: A potential approach for agronomic crops: A review. Plant Science Today, 8(1), 190–198. https://doi.org/10.14719/pst.2021.8.1.1045
Eviati, Sulaeman, Herawaty, L., Anggria, L., Usman, Tantika, H.E., Prihatini, R., & Wuningrum, P. (2023). Petunjuk Teknis Analisis Kimia Tanah, Tanaman, Air dan Pupuk (Edisi ke-3). Balai Pengujian Standar Instrumen Tanah dan Pupuk, Bogor. https://repository.pertanian.go.id/handle/123456789/24650
Fu, J. (2024). Biochemical pathways of starch synthesis in cassava: Genetic bases and breeding implications. Plant Gene and Trait, 15(3), 108–117. https://doi.org/10.5376/pgt.2024.15.0012
Giri, A., Verma, R., & Mehta, S. (2025). Biofertilizers: A sustainable solution for enhanced crop yield and soil health in modern agriculture. International Journal of Plant & Soil Science, 37(8), 562–576. https://doi.org/10.9734/ijpss/2025/v37i85656
Hridya, A.C., Byju, G., & Misra, R.S. (2014). Effects of microbial inoculations on soil chemical, biochemical and microbial biomass carbon of cassava (Manihot esculenta Crantz) growing Vertisols. Archives of Agronomy and Soil Science, 60(2), 239–249. https://doi.org/10.1080/03650340.2013.791023
Kaul, S., Sharma, S., Apra, & Dhar, M.K. (2019). Phosphate-solubilising fungi and their potential role in sustainable agriculture. In B. Giri, R. Prasad, Q.S. Wu, & A. Varma (Eds.), Biofertilizers for sustainable agriculture and environment (pp. 371–393). Springer. https://doi.org/10.1007/978-3-030-18933-4_17
Opoku-Agyemang, F., Amissah, J.N., Owusu-Nketia, S., Ofori, P.A., & Notaguchi, M. (2024). Optimization of cassava (Manihot esculenta Crantz) grafting technique to enhance its adoption in cassava cultivation. MethodsX, 13, 102904. https://doi.org/10.1016/j.mex.2024.102904
Otaiku, A.A., Mmom, P.C., & Ano, A.O. (2019). Biofertilizer impacts on cassava (Manihot esculenta Crantz) rhizosphere: Crop yield and growth components, Igbariam, Nigeria – Paper 1. World Journal of Agriculture and Soil Science, 3(5), 000575. https://doi.org/10.33552/WJASS.2019.03.000575
Pii, Y., Penn, A., Terzano, R., Crecchio, C., Mimmo, T., & Cesco, S. (2015). Plant-microorganism-soil interactions influence the Fe availability in the rhizosphere of cucumber plants. Plant Physiology and Biochemistry, 87, 45–52. https://doi.org/10.1016/j.plaphy.2014.12.014
Purwanto, B.H., & Alam, S. (2020). Impact of intensive agricultural management on carbon and nitrogen dynamics in the humid tropics. Soil Science and Plant Nutrition, 66(1), 50–59. https://doi.org/10.1080/00380768.2019.1705182
Pusdatin Kementan (Pusat Data dan Sistem Informasi Pertanian, Kementerian Pertanian RI). (2020). Outlook Komoditas Ubi Kayu 2020. Kementerian Pertanian RI, Jakarta.
Radjit, B., & Prasetiaswati, N. (2011). Potensi hasil umbi dan kadar pati pada beberapa varietas ubikayu dengan sistim sambung (Mukibat). Buana Sains, 11(1), 35–44.
Saleh, N., Taufiq, A., Widodo, Y., Sundari, T., Gusyana, D., Rajagukguk, R.P., & Suseno, S.A. (2016). Pedoman budi daya ubi kayu di Indonesia. IAARD Press.
Shahwar, D., Mushtaq, Z., Mushtaq, H., Alqarawi, A.A., Park, Y., Alshahrani, T.S., & Faizan, S. (2023). Role of microbial inoculants as bio fertilizers for improving crop productivity: A review. Heliyon, 9(6), e16134. https://doi.org/10.1016/j.heliyon.2023.e16134
Timofeeva, A.M., Galyamova, M.R., & Sedykh, S.E. (2023). Plant growth-promoting soil bacteria: Nitrogen fixation, phosphate solubilization, siderophore production, and other biological activities. Plants, 12(24), 4074. https://doi.org/10.3390/plants12244074
Triana, M., Yusnita, Y., Utomo, S. D., Hapsoro, D., & Yelli, F. (2025). Effect of Indole Butyric Acid (IBA) application and spliced grafting on the growth of garuda clone cassava. Jurnal Teknik Pertanian Lampung, 14(6), 2046–2056. https://doi.org/10.23960/jtepl.v14i6.2046-2056
Utomo, S.D., Laksmana, D., & Edy, A. (2025). Cassava cultivar development toward attainment of zero hunger: Variation of morphological and agronomic characters and selection on F1 population. SDGs Review, 5(6), e06928. https://doi.org/10.47172/2965-730X.SDGsReview.v5.n06.pe06928
Waluyo, T. (2020). Pemanfaatan hormon tumbuh organik untuk meningkatkan produktivitas singkong hasil eksplorasi seleksi bibit unggul. Jurnal Ilmu dan Budaya, 41(70). https://doi.org/10.47313/jib.v41i70.923
Zhang, J., Liu, Y.-X., Zhang, N., Hu, B., Jin, T., Xu, H., Qin, Y., Yan, P., Zhang, X., Guo, X., Hui, J., Cao, S., Wang, X., Wang, C., Wang, H., Qu, B., Fan, G., Yuan, L., Garrido-Oter, R., Chu, C., & Bai, Y. (2019). NRT1.1B is associated with root microbiota composition and nitrogen use in field-grown rice. Nature Biotechnology, 37, 676–684. https://doi.org/10.1038/s41587-019-0104-4

This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.



ARTICLE TEMPLATE