Biochar-Assisted Anaerobic Digestion: A Bibliometric Analysis of Publication Trends, Global Collaboration, and Emerging Research Themes
Abstract
Biochar is widely used to enhance anaerobic digestion (AD) and biogas production, yet bibliometric analyses of this field remain limited. This study analyzes publication trends, scientific productivity, international collaboration, and the evolution of research in biochar-assisted AD. Scopus data were retrieved using TITLE-ABS-KEY [Biochar AND ("Anaerobic Digestion" OR "Anaerobic Digester" OR Biogas OR Biomethane OR "Methane Yield" OR Methanogenesis)]. The search covered publications from January 2012 to October 2025 and was conducted on 16 November 2025. A total of 374 articles were analyzed using Biblioshiny and VOSviewer. Publications grew by 40.74% annually, reaching 85 articles in 2025. China produced the largest share of publications (149 articles; 39.8%), whereas Hong Kong and Ireland showed higher proportions of international collaboration and higher average citations per article. Keyword co-occurrence analysis identified four thematic clusters, whereas conceptual structure analysis identified five. Research focus has shifted from biochar properties and AD performance to microbial interactions and Direct Interspecies Electron Transfer (DIET), with engineered and magnetic biochar emerging as topics of interest. These findings indicate that biochar-assisted AD research is increasingly focused on mechanistic understanding and material development. Future studies should prioritize standardized reporting, pilot-scale validation, techno-economic assessment, and evaluation of biochars derived from local biomass.
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Abramo, G., Tindaro, C., & Ciriaco, A.D. 2011. Assessing the varying level of impact measurement accuracy as a function of the citation window length. Journal of informetrics, 5(4):659-667. https://doi.org/10.1016/j.joi.2011.06.004
Aria, M., & Cuccurullo, C. (2017). Bibliometrix: An R-tool for comprehensive science mapping analysis. Journal of Informetrics, 11(4), 959–975. https://doi.org/10.1016/j.joi.2017.08.007
Arruda, H., Silva, E. R., Lessa, M., Proença Jr, D., & Bartholo, R. (2022). VOSviewer and Bibliometrix. Journal of the Medical Library Association, 110(3):392-395. https://doi.org/10.5195/jmla.2022.1434
Belter, C.W. (2015). Bibliometric indicators: Opportunities and limits. Journal of the Medical Library Association, 103(4), 219–221. https://doi.org/10.3163/1536-5050.103.4.014
Berzal de Frutos, O., Götze, M., Pidou, M., & Bajón Fernández, Y. (2023). Anaerobic co-digestion of sewage sludge and trade wastes: Beneficial and inhibitory effects of individual constituents. Processes, 11(2), Article 519. https://doi.org/10.3390/pr11020519
Borges, L.F.F., Fischer, B., Mazoni, A., & Bascur, J.P. (2025). The complex interplay between international collaboration and scientific impact: evidence from the Scopus database. Cogent Education, 12(1), Article 2563708. https://doi.org/10.1080/2331186X.2025.2563708
Bu, J., Hu, B.B., Wu, H.Z., Tang, L.L., Liu, D.F., Xu, X.J., & Chen, S.S. (2022). Improved methane production with redox-active/conductive biochar amendment by establishing spatial ecological niche and mediating electron transfer. Bioresource Technology, 353, Article 127123. https://doi.org/10.1016/j.biortech.2022.127123
Chang, P.Y., Chan, Y.J., Arumugasamy, S.K., Wan, Y.K., & Lim, J.W. (2025). Optimisation of anaerobic digestion of palm oil mill effluent with biochar addition: Synergistic application of artificial neural network and response surface methodology. Fuel, 398, Article 135514. https://doi.org/10.1016/j.fuel.2025.135514
Cheng, D., Ngo, H.H., Guo, W., Chang, S.W., Nguyen, D.D., Nguyen, Q.A., Zhang, J., & Liang, S. (2021). Improving sulfonamide antibiotics removal from swine wastewater by supplying a new pomelo peel derived biochar in an anaerobic membrane bioreactor. Bioresource Technology, 319, Article 124160. https://doi.org/10.1016/j.biortech.2020.124160
Chiappero, M., Fiore, S., & Berruti, F. (2022). Impact of biochar on anaerobic digestion: Meta-analysis and economic evaluation. Journal of Environmental Chemical Engineering, 10(6), Article 108870. https://doi.org/10.1016/j.jece.2022.108870
Deng, X., Teng, F., & Wang, P. (2024). Exploring negative emission potential of biochar to achieve carbon neutrality goal in China. Nature Communications, 15, Article 1085. https://doi.org/10.1038/s41467-024-45314-y
Ding, J., Zhen, F., Kong, X., Hu, Y., Zhang, Y., & Gong, L. (2024). Effect of biochar in modulating anaerobic digestion performance and microbial structure community of different inoculum sources. Fermentation, 10(3), Article 151. https://doi.org/10.3390/fermentation10030151
Donthu, N., Kumar, S., Mukherjee, D., Pandey, N., & Lim, W.M. (2021). How to conduct a bibliometric analysis: An overview and guidelines. Journal of Business Research, 133, 285–296. https://doi.org/10.1016/j.jbusres.202
Duarte, I., Santos, H. G. G., & Rodrigues, M. L. (2025). A general analysis of the impact of international collaboration on the citation indices of scientific publications from 60 institutions across five continents. Anais da Academia Brasileira de Ciências, 97(1), e20241035. https://doi.org/10.1590/0001-3765202520241035
García-Prats, M., González, D., & Sánchez, A. (2024). Characterization of biochars of different origin and application to the anaerobic digestion of source-selected organic fraction of municipal solid waste under batch conditions and at different dosages. Frontiers in Chemical Engineering, 6, Article 1384495. https://doi.org/10.3389/fceng.2024.1384495
García-Prats, M., González, D., & Sánchez, A. (2026). Current trends and future prospects of biochar use to improve anaerobic digestion: An up-to-date critical review. Molecules, 31(3), Article 503. https://doi.org/10.3390/molecules31030503
Jiang, Q., Chen, Y., Yu, S., Zhu, R., Zhong, C., Zou, H., Gu, L., & He, Q. (2020). Effects of citrus peel biochar on anaerobic co-digestion of food waste and sewage sludge and its direct interspecies electron transfer pathway study. Chemical Engineering Journal, 398, Article 125619. https://doi.org/10.1016/j.cej.2020.125619
Jin, H.-Y., He, Z.-W., Ren, Y.-X., Zou, Z.-S., Tang, C.-C., Zhou, A.-J., Liu, W., Li, Z., & Wang, A. (2024). Revealing the roles of biochar derived from iron-rich fermented sludge residue in anaerobic digestion. Chemical Engineering Journal, 481, 148566. https://doi.org/10.1016/j.cej.2023.148566
Kaur, G., Johnravindar, D., & Wong, J.W.C. (2020). Enhanced volatile fatty acid degradation and methane production efficiency by biochar addition in food waste-sludge co-digestion: A step towards increased organic loading efficiency in co-digestion. Bioresource Technology, 307, 123211. https://doi.org/10.1016/j.biortech.2020.123211
Kemka, U.N., Ogbulie, T.E., Oguzie, K., Akalezi, C.O., & Oguzie, E.E. (2024). Impact of substrate heterogeneity on anaerobic co-digestion process: a review. EQA - International Journal of Environmental Quality, 62, 1–17. https://doi.org/10.6092/issn.2281-4485/18498
Kumar, M., Dutta, S., You, S., Luo, G., Zhang, S., Show, P.L., Sawarkar, A.D., Singh, L., & Tsang, D.C.W. (2021). A critical review on biochar for enhancing biogas production from anaerobic digestion of food waste and sludge. Journal of Cleaner Production, 305, 127143. https://doi.org/10.1016/j.jclepro.2021.127143
Li, X., Cen, K., Wang, L., Jia, D., Zhu, X., & Chen, D. (2024). Co-pyrolysis of cellulose and lignin: Effects of pyrolysis temperature, residence time, and lignin percentage on the properties of biochar using response surface methodology. Industrial Crops and Products, 220, 119280. https://doi.org/10.1016/j.indcrop.2024.119280
Lim, W.M., Kumar, S., & Donthu, N. (2024). How to combine and clean bibliometric data and use bibliometric tools synergistically: Guidelines using metaverse research. Journal of Business Research, 182, 114760. https://doi.org/10.1016/j.jbusres.2024.114760
Lü, F., Luo, C., Shao, L., & He, P. (2016). Biochar alleviates combined stress of ammonium and acids by firstly enriching Methanosaeta and then Methanosarcina. Water Research, 90, 34–43. https://doi.org/10.1016/j.watres.2015.12.029
Masebinu, S.O., Akinlabi, E.T., Muzenda, E., & Aboyade, A.O. (2019). A review of biochar properties and their roles in mitigating challenges with anaerobic digestion. Renewable and Sustainable Energy Reviews, 103, 291–307. https://doi.org/10.1016/j.rser.2018.12.048
Meng, Q.-B., He, Z.-W., Li, Z.-H., Tang, C.-C., Zhou, A.-J., Liang, B., Liu, W., Ren, Y.-X., & Wang, A. (2026). Iron redox cycling drives enhanced methanogenesis in magnetic biochar-mediated anaerobic digestion of waste-activated sludge. Environmental Science and Ecotechnology, 29, 100660. https://doi.org/10.1016/j.ese.2026.100660
Palamae, S., Palachum, W., Chisti, Y., & Choorit, W. (2014). Retention of hemicellulose during delignification of oil palm empty fruit bunch (EFB) fiber with peracetic acid and alkaline peroxide. Biomass and Bioenergy, 66, 127–139. https://doi.org/10.1016/j.biombioe.2014.01.037
Parra-Orobio, B.A., Soto-Paz, J., Oviedo-Ocaña, E.R., Vali, S.A., & Sánchez, A. (2023). Advances, trends and challenges in the use of biochar as an improvement strategy in the anaerobic digestion of organic waste: a systematic analysis. Bioengineered, 14(1), 2252191. https://doi.org/10.1080/21655979.2023.2252191
Pei, S., Fan, X., Qiu, C., Liu, N., Li, F., Li, J., Qi, L., & Wang, S. (2024). Effect of biochar addition on the anaerobic digestion of food waste: microbial community structure and methanogenic pathways. Water Science and Technology, 90(3), 894–907. https://doi.org/10.2166/wst.2024.199
Qin, Y., Yin, X., Xu, X., Yan, X., Bi, F., & Wu, W. (2020). Specific surface area and electron donating capacity determine biochar's role in methane production during anaerobic digestion. Bioresource Technology, 303, 122971. https://doi.org/10.1016/j.biortech.2020.122971
Qu, J., Wang, H., Wang, K., Yu, G., Ke, B., Yu, H.Q., Ren, H., Zheng, X., Li, J., Gao, S., & Gong, H. (2019). Municipal wastewater treatment in China: Development history and future perspectives. Frontiers of Environmental Science & Engineering, 13(6), 88. https://doi.org/10.1007/s11783-019-1172-x
Shi, Z., Xing, K., & Chow, C.W.K. (2024). Current trends and future directions of global research on wastewater to energy: a bibliometric analysis and review. Environmental Science and Pollution Research, 31(14), 20792–20813. https://doi.org/10.1007/s11356-024-32560-2
Sicchieri, I.M.B., Quadros, T.C.F.d., Bortoloti, M.A., Souza, A.T.d., Nogueira, H.P., Araki, K., Fernandes, F., & Kuroda, E.K. (2025). Biochars from waste as an additive material in anaerobic co-digestion: Characterization and application in batch and semi-continuous systems. Fuel, 381, 133342. https://doi.org/10.1016/j.fuel.2024.133342
Sivakumar, P., Saravanane, R., Mohan, S., & Sankar, B. (2025). Biochar as a catalyst for methane enhancement in anaerobic digestor containing cow dung, food waste, and rice straw: An experimental and statistical study. Cleaner Waste Systems, 12, 100388. https://doi.org/10.1016/j.clwas.2025.100388
Sjögårde, P., & Didegah, F. (2022). The association between topic growth and citation impact of research publications. Scientometrics, 127(4), 1903–1921. https://doi.org/10.1007/s11192-022-04293-x
Sugiarto, Y., Sunyoto, N.M.S., Zhu, M., Jones, I., & Zhang, D. (2021). Effect of biochar addition on microbial community and methane production during anaerobic digestion of food wastes: The role of minerals in biochar. Bioresource Technology, 323, 124597. https://doi.org/10.1016/j.biortech.2020.124597
Sun, Z., Feng, L., Li, Y., Han, Y., Zhou, H., & Pan, J. (2022). The role of electrochemical properties of biochar to promote methane production in anaerobic digestion. Journal of Cleaner Production, 370, 133485. https://doi.org/10.1016/j.jclepro.2022.133485
Tahamtan, I., Afshar, A.S., & Ahamdzadeh, K. (2016). Factors affecting number of citations: a comprehensive review of the literature. Scientometrics, 107(3), 1195–1225. https://doi.org/10.1007/s11192-016-1889-2
Valentin, M.T., Luo, G., & Białowiec, A. (2023). Direct interspecies electron transfer mechanisms of a biochar-amended anaerobic digestion: a review. Biotechnology for Biofuels and Bioproducts, 16(1), 146. https://doi.org/10.1186/s13068-023-02391-3
Van Eck, N.J., & Waltman, L. (2010). Software survey: VOSviewer, a computer program for bibliometric mapping. Scientometrics, 84(2), 523–538. https://doi.org/10.1007/s11192-009-0146-3
Wagner, C.S., Whetsell, T.A., & Leydesdorff, L. (2017). Growth of international collaboration in science: revisiting six specialties. Scientometrics, 110(3), 1633–1652. https://doi.org/10.1007/s11192-016-2230-9
Wang, G., Gao, X., Li, Q., Zhao, H., Liu, Y., Wang, X.C., & Chen, R. (2020). Redox-based electron exchange capacity of biowaste-derived biochar accelerates syntrophic phenol oxidation for methanogenesis via direct interspecies electron transfer. Journal of Hazardous Materials, 390, 121726. https://doi.org/10.1016/j.jhazmat.2019.121726
Wei, W., Guo, W., Ngo, H.H., Mannina, G., Wang, D., Chen, X., Liu, Y., Peng, L., & Ni, B.J. (2020). Enhanced high-quality biomethane production from anaerobic digestion of primary sludge by corn stover biochar. Bioresource Technology, 306, 123159. https://doi.org/10.1016/j.biortech.2020.123159
Wu, P., Fu, Y., & Chen, W. (2024). Analyzing the trends and hotspots of biochar’s applications in agriculture, environment, and energy: a bibliometrics study for 2022 and 2023. Biochar, 6(1), 78. https://doi.org/10.1007/s42773-024-00370-x
Wu, P., Singh, B.P., Wang, H., Jia, Z., Wang, Y., & Chen, W. (2023). Bibliometric analysis of biochar research in 2021: a critical review for development, hotspots and trend directions. Biochar, 5(1), 6. https://doi.org/10.1007/s42773-023-00204-2
Xu, H., Wang, L., & Xu, D. (2024). Global publication productivity and research trends on recurrent ovarian cancer: a bibliometric study. Frontiers in Oncology, 14, 1422213. https://doi.org/10.3389/fonc.2024.1422213
Yang, S., Chen, Z., & Wen, Q. (2021). Impacts of biochar on anaerobic digestion of swine manure: Methanogenesis and antibiotic resistance genes dissemination. Bioresource Technology, 324, 124679. https://doi.org/10.1016/j.biortech.2021.124679
Yuan, Y., Bolan, N., Prévoteau, A., Vithanage, M., Biswas, J.K., Ok, Y.S., & Wang, H. (2017). Applications of biochar in redox-mediated reactions. Bioresource Technology, 246, 271–282. https://doi.org/10.1016/j.biortech.2017.06.154
Zhang, J., Liu, H., Wu, J., Chen, C., Ding, Y., Liu, H., & Zhou, Y. (2025). Rethinking the biochar impact on the anaerobic digestion of food waste in bench-scale digester: Spatial distribution and biogas production. Bioresource Technology, 418, 132115. https://doi.org/10.1016/j.biortech.2025.132115
Zhang, L., Lim, E.Y., Loh, K.-C., Ok, Y.S., Lee, J.T.E., Shen, Y., Wang, C.-H., Dai, Y., & Tong, Y.W. (2020). Biochar enhanced thermophilic anaerobic digestion of food waste: Focusing on biochar particle size, microbial community analysis and pilot-scale application. Energy Conversion and Management, 209, 112654. https://doi.org/10.1016/j.enconman.2020.112654
Zhang, L., Tsui, T.-H., Tong, Y.W., Aggarangsi, P., & Liu, R. (2024). Applying current-carrying-coil-based magnetic field (CCC-MF) to promote anaerobic digestion of chicken manure: Performance evaluation, mitigation of ammonia inhibition, microbial community analysis, and pilot-scale validation. Energy Conversion and Management, 300, 117908. https://doi.org/10.1016/j.enconman.2023.117908
Zhou, W., Mazarji, M., Li, M., Li, A., Wang, Y., Yang, Y., Lee, J.T.E., Rene, E.R., Yuan, X., & Pan, J. (2024). Exploring magnetic nanomaterials with a focus on magnetic biochar in anaerobic digestion: from synthesis to application. Biochar, 6, 63. https://doi.org/10.1007/s42773-024-00354-x

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