Enkapsulasi bakteri asam laktat dari tempoyak asal Jambi: Pengaruh konsentrasi alginat [Encapsulation of lactic acid bacteria isolated from fermented durian (tempoyak) in Jambi: Influence of alginate concentration]

Authors

  • Addion Nizori Jambi University
  • Nanda Prayogi
  • Ika Gusriani
  • Lavlinesia Lavlinesia
  • Arzita Arzita

DOI:

https://doi.org/10.23960/jtihp.v26i1.56-64
Abstract View: 1035

Keywords:

Emulsification, alginate, encapsulation, lactic acid bacteria, tempoyak

Abstract

This study aimed to investigate the characteristics and the viability of encapsulated lactic acid bacteria (LAB) from fermented durian from Jambi using alginate concentration as wall material. The encapsulation method used the emulsification technique where LAB in alginate capsules was formed by the formation of water in oil emulsion. This study used a completely randomized design with various alginate concentrations as the treatments with concentrations: 1%, 3%, 5%, 7%, and 9%, respectively. The result showed that alginate concentration on LAB microcapsules was very significant in yield, pH, and heat resistance. All treatment concentrations provided high LAB viability, so alginate is effective for wall material LAB encapsulation with emulsification techniques.  The best LAB microcapsule was 7% of alginate concentration with value of yield 5,60 %, viability of LAB log 12,43 CFU/g, pH 5,22, heat resistant on 50ºC (log 12, 46 CFU/g), 60ºC (log 12,45 CFU/g), 70ºC (log 12,35 CFU/g).  


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References

Chen, M.Y., W. Zheng., Q.Y. Dong, Z.H., Li, L.E., Shi, and Z.X. Tang. (2014). Activity of Encapsulated Lactobacillus bulgaricus in Alginatewhey Protein Microspheres. Braz. Arch. Biol. Technol, 57(5): 736-741.

De Cássia Sousa Mendes, D., Asquieri, E. R., Batista, R. D., de Morais, C. C., Ramirez Ascheri, D. P., de Macêdo, I. Y. L., & de Souza Gil, E. (2021). Microencapsulation of jabuticaba extracts (Myrciaria cauliflora): Evaluation of their bioactive and thermal properties in cassava starch biscuits. LWT, 137, 110460. https://doi.org/https://doi.org/10.1016/j.lwt.2020.110460

Ding, W. K., & Shah, N. P. (2008). Survival of Free and Microencapsulated Probiotic Bacteria in Orange and Apple Juices. International Food Research Journal, 15(2), 219–232.

Gebara, C., Chaves, K. S., Ribeiro, M. C. E., Souza, F. N., Grosso, C. R. F., & Gigante, M. L. (2013). Viability of Lactobacillus acidophilus La5 in pectin–whey protein microparticles during exposure to simulated gastrointestinal conditions. Food Research International, 51(2), 872–878. https://doi.org/https://doi.org/10.1016/j.foodres.2013.02.008

Haffner, F. B., & Pasc, A. (2018). Freeze-dried alginate-silica microparticles as carriers of probiotic bacteria in apple juice and beer. LWT, 91, 175–179. https://doi.org/https://doi.org/10.1016/j.lwt.2018.01.050

Hansen, L. T., Allan-Wojtas, P. M., Jin, Y.-L., & Paulson, A. T. (2002). Survival of Ca-alginate microencapsulated Bifidobacterium spp. in milk and simulated gastrointestinal conditions. Food Microbiology, 19(1), 35–45. https://doi.org/https://doi.org/10.1006/fmic.2001.0452

Holkem, A. T., Raddatz, G. C., Barin, J. S., Moraes Flores, É. M., Muller, E. I., Codevilla, C. F., Jacob-Lopes, E., Ferreira Grosso, C. R., & de Menezes, C. R. (2017). Production of microcapsules containing Bifidobacterium BB-12 by emulsification/internal gelation. LWT - Food Science and Technology, 76, 216–221. https://doi.org/https://doi.org/10.1016/j.lwt.2016.07.013

Khalid, K., 2011. An overview of lactic acid bacteria. International Journal of Biosciences. 1 (3); 1-13.

Kim, S. J., S. Y. Cho., S. H. Kim., O. J. Song., I.S. Shin., D.S. Cha and H. J. Park. (2008). Effect of microencapsulation on viability and other characteristics in Lactobacillus acidophilus ATCC 43121. LWT-Food Sci and Technol. 41: 493-500.

Krasaekoopt, W., Bhandari, B., & Deeth, H. (2003). Evaluation of encapsulation techniques of probiotics for yoghurt. International Dairy Journal, 13(1), 3–13. https://doi.org/https://doi.org/10.1016/S0958-6946(02)00155-3

Lee, Y., Y.R. Ji,. S. Lee., M.J. Choi and Y. Cho. (2019). Microencapsulation of probiotic Lactobacillus acidophilus kbl409 by extrusion technology to enhance survival under simulated intestinal and freeze-drying conditions. Journal of Microbiol. Biotechnology 29, 721–730.

Mandal, S., Puniya, A. K., & Singh, K. (2006). Effect of alginate concentrations on survival of microencapsulated Lactobacillus casei NCDC-298. International Dairy Journal, 16(10), 1190–1195. https://doi.org/https://doi.org/10.1016/j.idairyj.2005.10.005

Marco, M. L., Heeney, D., Binda, S., Cifelli, C. J., Cotter, P. D., Foligné, B., Gänzle, M., Kort, R., Pasin, G., Pihlanto, A., Smid, E. J., & Hutkins, R. (2017). Health benefits of fermented foods: microbiota and beyond. Current Opinion in Biotechnology, 44, 94–102. https://doi.org/https://doi.org/10.1016/j.copbio.2016.11.010

Muchsiri, M., Basuni, H., Agus, W dan Pambayun, R. (2015). Pengaruh kosentrasi natrium alginate dan jenis BAL terhadap viabilitas sel enkapsulasi probiotik BAL. Jurnal Agrin Vol 19 (2).http://dx.doi.org/10.20884/1.agrin.2015.19.2.243

Nizori, A., Sukendra, A., & Mursyid, S. (2019). Antimicrobial activity of lactic acid bacteria isolated from fermented durian flesh (tempoyak) against pathogenic and spoilage bacteria during storage. IOP Conference Series: Earth and Environmental Science, 347(1). https://doi.org/10.1088/1755-1315/347/1/012053

Nizori, A., Bui, L. T. T., Jie, F., & Small, D. M. (2018). Impact of varying hydrocolloid proportions on encapsulation of ascorbic acid by spray drying. International Journal of Food Science and Technology, 53(6). https://doi.org/10.1111/ijfs.13699

Nizori, A., Bui, L. T. T., Jie, F., & Small, D. M. (2020). Spray-drying microencapsulation of ascorbic acid: impact of varying loading content on physicochemical properties of microencapsulated powders. Journal of the Science of Food and Agriculture, 100(11), 4165–4171.

Nizori, A. (2018). The Properties of Jambi tempoyak; The effects of salts concentration. Indonesian Food Science & Technology Journal, 1(1), 27–30. https://doi.org/10.22437/ifstj.v1i1.5007

Oguntoye, M. A., Ezekiel, O. O., & Oridupa, O. A. (2021). Viability of Lactobacillus rhamnosus GG in provitamin A cassava hydrolysate during fermentation, storage, in vitro and in vivo gastrointestinal conditions. Food Bioscience, 40, 100845. https://doi.org/https://doi.org/10.1016/j.fbio.2020.100845

Oxoid (1998). The Oxoid Manual. 8th ed. Complied by E.Y Bridson (Former Technical director of Oxoid).

Rajagukguk, Y. V., & Arnold, M. (2021). Tempoyak: Fermented durian paste of Malay ethnic and its functional properties. International Journal of Gastronomy and Food Science, 23, 100297. https://doi.org/https://doi.org/10.1016/j.ijgfs.2020.100297

Ruiz Rodríguez, L. G., Zamora Gasga, V. M., Pescuma, M., Van Nieuwenhove, C., Mozzi, F., & Sánchez Burgos, J. A. (2021). Fruits and fruit by-products as sources of bioactive compounds. Benefits and trends of lactic acid fermentation in the development of novel fruit-based functional beverages. Food Research International, 140, 109854. https://doi.org/https://doi.org/10.1016/j.foodres.2020.109854

Samborska, K., Boostani, S., Geranpour, M., Hosseini, H., Dima, C., Khoshnoudi-Nia, S., Rostamabadi, H., Falsafi, S. R., Shaddel, R., Akbari-Alavijeh, S., & Jafari, S. M. (2021). Green biopolymers from by-products as wall materials for spray drying microencapsulation of phytochemicals. Trends in Food Science & Technology, 108, 297–325.

Saputro, A. D., Van de Walle, D., & Dewettinck, K. (2020). Physicochemical properties of coarse palm sap sugars as natural alternative sweetener. Food Bioscience, 38, 100780. https://doi.org/https://doi.org/10.1016/j.fbio.2020.100780

Sudarmadji, S., H. Bambang dan Suhardi. (1997). Prosedur Analisa Untuk Bahan Makanan dan Pertanian. Penerbit Angkasa. Bandung.

Salsac, A. V. L., Zhang, and J. M. Gherbezz. (2009). Measurement of mechanical properties of alginate eads using ultrasound. 19. Eme congre’es franc, ais de M’ecanique Marseille. 9(2): 24-28.

Usfah A. P. J. (2015). Enkapsulasi Lactobacillus dengan Metode Emulsi dan Aplikasinya Pada Dodol Sirsak. (Thesis). Sekolah Pascasarjana. Institut Pertanian Bogor. Bogor. http://repository.ipb.ac.id/handle/123456789/76716.

Wandrey, C., Bartkowiak, A., & Harding, S. E. (2010). Materials for Encapsulation BT - Encapsulation Technologies for Active Food Ingredients and Food Processing (N. J. Zuidam & V. Nedovic (eds.); pp. 31–100). Springer New York. https://doi.org/10.1007/978-1-4419-1008-0_3

Wasnin, R. M., Karim, M. S. A., & Ghazali, H. M. (2014). Effect of temperature-controlled fermentation on physico-chemical properties and lactic acid bacterial count of durian (Durio zibethinus Murr.) pulp. Journal of Food Science and Technology, 51(11), 2977–2989. https://doi.org/10.1007/s13197-012-0869-7

Zhu, X. Y., & Liu, F. (2017). Probiotics as an adjuvant treatment in Helicobacter pylori eradication therapy. Journal of Digestive Diseases, 18(4), 195–202. https://doi.org/https://doi.org/10.1111/1751-2980.12466

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Published

2021-03-09

How to Cite

Nizori, A., Prayogi, N., Gusriani, I., Lavlinesia, L., & Arzita, A. (2021). Enkapsulasi bakteri asam laktat dari tempoyak asal Jambi: Pengaruh konsentrasi alginat [Encapsulation of lactic acid bacteria isolated from fermented durian (tempoyak) in Jambi: Influence of alginate concentration]. Jurnal Teknologi &Amp; Industri Hasil Pertanian, 26(1), 56–64. https://doi.org/10.23960/jtihp.v26i1.56-64